Model-based characterization of metabolism of the recombinant adeno-associated virus (rAAV) production via Human Embryonic Kidney (HEK293) cells

Model-based characterization of metabolism of the recombinant adeno-associated virus (rAAV) production via Human Embryonic Kidney (HEK293) cells | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Model-based characterization of metabolism of the recombinant adeno-associated virus (rAAV) production via Human Embryonic Kidney (HEK293) cells Somaiyeh Khodadadi Karimvand, Miroslava Cuperlovic-Culf, Amine A. Kamen, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4594915/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In this study, we present a kinetic-metabolic model describing adeno-associated virus (AAV) production via Hek293 cells, encompassing the main metabolic pathways, namely glycolysis, tricarboxylic acid cycle (TCA), pyruvate fates, the pentose phosphate pathway, anaplerotic reaction, amino acid metabolism, nucleotides synthesis, biomass synthesis and the metabolic pathways of protein synthesis of AAV (capsid and Rep proteins). For the modelling, Michaelis-Menten kinetic is assumed to define the metabolic model. A dataset from bioreactor cultures containing metabolite profiles of adeno-associated virus 6 (AAV6) production via triple transient transfection at low cell density culture, including concentration profiles of glutamine, glutamic acid, glucose, lactate, and ammonium, is utilized for fitting and computing the model parameters. The model resulting from the adjusted parameters well defines the experimental data. Subsequently, a Sobol-based global sensitivity analysis procedure is applied to determine the most sensitive parameters in the final model. Adeno-associated viruses Michaelis-Menten kinetic Metabolic model Global Sensitivity analysis Optimization Model fitting Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4594915","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":323515511,"identity":"4abf33d3-267d-411e-bc72-677e36da9608","order_by":0,"name":"Somaiyeh Khodadadi Karimvand","email":"","orcid":"","institution":"University of Ottawa","correspondingAuthor":false,"prefix":"","firstName":"Somaiyeh","middleName":"Khodadadi","lastName":"Karimvand","suffix":""},{"id":323515512,"identity":"ed1e1e8d-b20a-48a5-ac1d-0965c24f42f2","order_by":1,"name":"Miroslava Cuperlovic-Culf","email":"","orcid":"","institution":"National Research Council Canada","correspondingAuthor":false,"prefix":"","firstName":"Miroslava","middleName":"","lastName":"Cuperlovic-Culf","suffix":""},{"id":323515513,"identity":"eb8253e3-fc42-4e41-b13d-bd262e6a76a7","order_by":2,"name":"Amine A. 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