A case of primary adenosquamous carcinoma of the liver.

A 73-year-old man complained of fever and chill, and was hospitalized for urinary tract infection at the local clinic. During hospitalization, hepatic mass was observed from ultrasonography (US) and was transferred to Chungnam National University Hospital for further investigation of the liver mass. He had a history of endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic sphincterotomy with stone extraction, due to cholangitis accompanying bile duct stone 6 years ago. He had neither specific family history nor history of smoking, as well as drinking. He was currently working in the field of agriculture. On admission, he was chronic ill looking with icteric sclera. He had a temperature of 36.2℃ and physical examination was unremarkable. Initial laboratory evaluation were as follows: WBC 7,880/mm 3 , Hb 13.1 g/dl, platelet 258,000/mm 3 , PT (prothrombin time) 12.5 sec (INR: 1.1), aPTT (partial thromboplastin time) 23.6 sec, total protein 7.5 mg/dl, Albumin 3.5 mg/dl, AST 13 IU/L, ALT 8 IU/L, ALP 151 IU/L, GGT (gamma glutamyl transferase) 89 IU/L, total bilirubin 1.4 mg/dl. HBsAg and HBsAb were both negative and HCV Ab was negative. CA 19-9 (carbohydrate antigen 19-9) and CEA (carcinoembryonic antigen) were 1,600 U/ml and 7.2 ng/ml. Abdominal computed tomography (CT) from the local clinic showed low-density mass with irregular margin on the left lobe. Hepatic mass showed rim enhancement on early phase with slow central enhancement on delayed phases. Distal intrahepatic duct dilatation and cirrhotic change were observed ( Fig. 1 ). Magnetic resonance imaging study showed a 5 cm-sized lobulated mass with dilatation of peritumoral intrahepatic duct (IHD) in the left segment of the liver, which was consistent with hepatic CCC and no evidence of any abnormal lymphadenopathy in significant size. Left hemihepatectomy and caudate lobectomy was conducted because the lesion seemed to be malignant. At operation, the tumor occupied II and III segment of the liver with periductal infiltration and intraductal growing to hilum. The specimen contained round yellow-gray cystic mass, measuring 5×5 cm. The tumor had free resection margin and lymph node labeled hepatoduodenal ligament and No. 8 had tumor metastasis. Postoperative TNM stage was pT1N1M0. Microscopically, the tumor was composed of adenocarcinoma component and squamous cell carcinoma component with a few keratin pearls. Thus, a diagnosis of hepatic ASC could be made. This tumor was composed of adenocarcinoma, squamous cell carcinoma, as well as transitional area, including the transformation of adenocarcinoma to squamous cell carcinoma ( Fig. 2 ). Postoperative adjuvant chemotherapy was performed, using 5-fluorouracil and leucovorin regimen. The patient remained well with no evidence of post operation complication or recurrence for 15 months since her operation.

Primary malignant tumor of the liver is categorized into hepatocellular carcinoma (HCC), CCC, sarcoma and lymphoma, and etc. The majority of primary malignant tumor of the liver is HCC and CCC. The incidence of CCC among primary malignant tumors of the liver is 3% to 5%, and the incidence of primary hepatic ASC among CCC is 2% to 3%. 1 Pianzola and Drut 3 described the first case of ASC of the liver in 1971 and computer MEDLINE search revealed about 65 histologically defined primary ASC cases reported in the literature from 1975 to the present. Kobayashi et al. 4 investigated ASC of the liver from reported cases in the literature, including their case. According to the study, hepatic ASC occurs about two fold more in men and the mean age of hepatic ASC is 62.4 years. Majority of patients complained of abdominal pain as the initial symptom. Abdominal pain, fever, and jaundice appear to be the three most frequent symptoms. Tumors located in the left lobe of the liver in half of the cases. Few primary hepatic ASC cases were observed in Kormed ( http://koreamed.org ) search from 2000 to present; the first case presented cholangiocarcinoma with abscess in the liver and was confirmed as ASC postoperatively. Also, the second case was first diagnosed as cholangiocarcinoma and was confirmed as ASC postoperatively. Next case was primary hepatic ASC with formation of tumor-colonic fistula. The forth case was primary hepatic ASC that developed metachronously with a colon adenocarcinoma ( Table 1 ). The pathogenesis of hepatic ASC remains unknown, with two major hypotheses proposed. Chronic inflammation may cause a metaplastic change in the biliary epithelium that leads to neoplasia. 5 - 7 This kind of chronic inflammation of the bile duct is usually associated with infection and/or stones and the lining epithelium of the hepatic cyst. In our case, we can speculate a possible role of persistent inflammation from patient's past history of endoscopic sphincterotomy with stone extraction, due to cholangitis accompanying bile duct stone. An alternative theory suggests that ASC of the liver arises from the squamous metaplasia of adenocarcinoma. 1 , 2 , 8 - 10 From this proposal, if metaplatic squamous epithelium subject to malignant transformation, the tumor tissue may be composed of normal epithelium. However, most tumor tissue is composed of squamous cell carcinoma and adenocarcinoma cells. Yeh et al. 2 investigated 12 patients with ASC of the liver and 29 reviewed cases. In their investigation, they speculated a possible role of hepatolithiasis in the malignant transformation superimposed on the squamous metaplasia of CCC. Thus, the second theory seems justifiable for describing the pathogenesis in most cases of ASC of the liver. In our case, microscopically, the tumor was composed of adenocarcinoma, squamous cell carcinoma, as well as transitional area, including the transformation of adenocarcinoma to squamous cell carcinoma. This is in accordance with the microscopic studies of four other hepatobiliary carcinoma of our institute. The adenocarcinoma component arising from the main biliary duct seemed to differentiate into the squamous cell carcinoma component. The treatment of choice for this tumor is hepatectomy, and some reports advocated radiation therapy. In our case, the patient was treated with curative operation and postoperative adjuvant chemotherapy. Preoperative diagnosis of hepatic ASC, using fine needle aspiration cytology or biopsy, is crucial for the prognostic prediction. However, due to peritoneal seeding concern and diverse diagnostic sensitivity, preoperative diagnosis of hepatic ASC is difficult. Primary hepatic ASC is initially diagnosed as other hepatic tumors, such as CCC, metastatic carcinoma, HCC, or hepatic cyst. As observed in the possible pathologic mechanism of ASC, our case initially shared features of CCC clinically. Although hepatic ASC is diagnosed postoperatively, postoperative adjuvant therapy, such as chemotherapy and/or radiotherapy should be started as soon as possible.

Adenosquamous carcinoma (ASC) is a tumor composed of both adenocarcinoma and squamous cell carcinoma components, especially adenocarcinoma containing significant amounts of unequivocal squamous carcinomatous elements, for example keratin and/or intercelluar bridges. Primary ASC is considered as a subtype of cholangiocelluar carcinoma (CCC). The incidence of ASC is rather common in the gall bladder, pancreas, stomach, thyroid, and large intestine, but hepatic ASC is very rare. Primary ASC of the liver has been reported mostly in a form of case studies in South Korea. ASC tends to present more aggressive clinicopathologic features and has a worse prognosis than CCC. 1 , 2 From five hepatobiliary ASC for the past 10 years in our institute, one primary hepatic ASC was observed. We describe a case of primary hepatic ASC, preoperatively diagnosed as CCC, along with investigating the literature of primary hepatic ASC and attempting to explore it clinicopathologically.