Expression of mRNA-TLR-5 Gene in Patients with Endometriosis using Real-time PCR in Tehran, Iran

M Raghibi, Maryam Raghibi, Maliheh Raghibi, Raghibi M, Abbas Morovvati, Morovvati A, Shakib P, Pegah Shakib, Aflatoonian R, Reza Aflatoonian, Ashkan Dirbaiyan, Dirbaiyan A
Recent advances in anti-infective drug discovery · 2024 · vol. 19(4) , pp. 300–306 · doi:10.2174/0127724344251369231212061409 · PMID:38213148 · W4390794980
article OA: closed CC0
Full text JSON View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

This study measured mRNA-TLR-5 gene expression in women with endometriosis and found significantly higher expression in ectopic endometriotic tissue compared to eutopic endometrium and control groups.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-07, 2026-07-09 · read from full text ⓘ

This study investigated mRNA expression of Toll-like receptor 5 (TLR-5) in women undergoing laparoscopic surgery in Tehran, Iran (2021), comparing ectopic endometrium from ovarian endometriotic cysts, eutopic endometrial mucosa, and normal endometrial samples from patients without infertility or endometriosis. After RNA extraction and cDNA synthesis, TLR-5 mRNA levels were measured using real-time PCR. TLR-5 expression was significantly higher in ectopic samples than in eutopic and control groups, with an additional significant difference between eutopic and control groups. The authors note the observed increase in ectopic TLR-5 expression may relate to reduced apoptosis and a microenvironment that supports survival and proliferation of cells in the peritoneal cavity, though no functional confirmation is described. This paper is centrally about endometriosis — it measures TLR-5 mRNA expression in ectopic versus eutopic endometrial tissue from endometriosis patients.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

BACKGROUND: Endometriosis is one of the common diseases of women, especially in reproductive age, and it is one of the most important causes of infertility in women. The aim of this study was to investigate the level of mRNA-TLR-5 expression in women with endometriosis. METHODS: The present study was performed in Nikan Hospital, Tehran, Iran, in 2021. The samples of endometrial mucosa for the eutopic group and an ovarian endometriotic cyst for the ectopic group were obtained from the patients who underwent laparoscopic surgery at the Fetal Infertility Center and were diagnosed with endometriosis. Normal endometrial samples were also obtained from patients who had no history of infertility and underwent laparoscopic TL surgery for reasons other than endometriosis such as ovarian cysts (control group). After RNA extraction and cDNA synthesis, TLR-5 gene expression was evaluated by the Real-Time PCR method. RESULTS: Based on the results of the comparison of TLR-5 gene expression in all three ectopic, eutopic endometrium, and control groups by Real-Time PCR, it was found that the TLR-5 gene expression is significantly higher in ectopic samples than in the other two groups, but there is a significant difference between two utopic and control groups. CONCLUSION: The increase in TLR-5 expression in the ectopic group can probably be a reason for reducing the apoptosis of cells entered into the peritoneal cavity and creating an environment for the survival and proliferation of these cells.
Full text 2,944 characters · extracted from oa-doi-fallback · 5 sections · click to expand

Abstract

Background: Endometriosis is one of the common diseases of women, especially in reproductive age, and it is one of the most important causes of infertility in women. The aim of this study was to investigate the level of mRNA-TLR-5 expression in women with endometriosis.

Methods

The present study was performed in Nikan Hospital, Tehran, Iran, in 2021. The samples of endometrial mucosa for the eutopic group and an ovarian endometriotic cyst for the ectopic group were obtained from the patients who underwent laparoscopic surgery at the Fetal Infertility Center and were diagnosed with endometriosis. Normal endometrial samples were also obtained from patients who had no history of infertility and underwent laparoscopic TL surgery for reasons other than endometriosis such as ovarian cysts (control group). After RNA extraction and cDNA synthesis, TLR-5 gene expression was evaluated by the Real-Time PCR method.

Results

Based on the results of the comparison of TLR-5 gene expression in all three ectopic, eutopic endometrium, and control groups by Real-Time PCR, it was found that the TLR-5 gene expression is significantly higher in ectopic samples than in the other two groups, but there is a significant difference between two utopic and control groups.

Conclusion

The increase in TLR-5 expression in the ectopic group can probably be a reason for reducing the apoptosis of cells entered into the peritoneal cavity and creating an environment for the survival and proliferation of these cells.

Keywords

Endometrial mucosa, gene expression, TLR-5 gene, real-time polymerase chain reaction (Real-Time PCR), glands and stroma, abdomen. [http://dx.doi.org/10.1016/j.ogc.2007.12.008] [PMID: 18319131] [http://dx.doi.org/10.1080/09513590.2019.1616688] [PMID: 31155975] [http://dx.doi.org/10.2217/whe.15.56] [PMID: 26439119] [http://dx.doi.org/10.1034/j.1600-0897.2003.00042.x] [PMID: 14506928] [http://dx.doi.org/10.1093/humupd/dmz018] [PMID: 31424502] [http://dx.doi.org/10.1016/j.bpobgyn.2018.01.010] [PMID: 29506962] [http://dx.doi.org/10.1111/jog.13559] [PMID: 29316073] [http://dx.doi.org/10.1007/s00404-021-06075-x] [PMID: 33928453] [http://dx.doi.org/10.1007/s12094-022-02999-1] [PMID: 36374404] [http://dx.doi.org/10.3892/mmr.2013.1755] [PMID: 24173432] [PMID: 16474276] [http://dx.doi.org/10.4049/jimmunol.167.4.1882] [PMID: 11489966] [http://dx.doi.org/10.1073/pnas.95.2.588] [PMID: 9435236] [http://dx.doi.org/10.1186/1477-7827-6-40] [http://dx.doi.org/10.1007/BF02773401] [http://dx.doi.org/10.1016/j.humimm.2011.08.015] [PMID: 21920397] [http://dx.doi.org/10.1016/j.molmed.2018.07.004] [PMID: 30054239] [http://dx.doi.org/10.1111/aji.13537] [PMID: 35263479] [http://dx.doi.org/10.1016/j.cyto.2009.08.010] [PMID: 19775907] [http://dx.doi.org/10.1093/humrep/deh775] [PMID: 15695310] [http://dx.doi.org/10.1016/j.jri.2009.09.008] [PMID: 19959241] [http://dx.doi.org/10.1159/000516543] [PMID: 34139707] [PMID: 20083854]

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-doi-fallback ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Condition tags

endometriosisinfertility

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (46)

Source provenance

crossref
last seen: 2026-07-28T06:55:12.375568+00:00
europepmc
last seen: 2026-08-03T06:10:56.557307+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pubmed
last seen: 2026-08-03T06:09:26.260000+00:00
unpaywall
last seen: 2026-08-03T06:41:53.707437+00:00
License: CC0 · commercial use OK