Evaluation of Antibacterial and Antibiofilm Activity of Essential Oils Against Isolated Bacteria From Milk Samples

Abstract The consumption of food contaminated with bacteria or their toxins can result in foodborne infections or illnesses. Every year, millions of individuals worldwide are affected by this. Food manufacturers now focus on food safety as a global health objective. Secondary metabolites from aromatic and medicinal plants can be identified as essential oils. There are numerous applications for these essential oils in the fragrance, cosmetic, and culinary industries. The chemical constituents of these essential oils vary significantly based on factors such as extraction techniques, bacterial endophytes, genetics, botanical origins, and geographic origin. In this study aims that isolation and identification of Biofilm forming bacteria from raw milk samples from the local vendors Pune, Maharashtra, India. In the present study Essential Oil (EO) and its constituents, which are known to possess several antimicrobial activities. The antibacterial properties of essential oil were investigated in vitro using the agar disc diffusion method. The microdilution method was employed to examine the minimal inhibitory concentration of essential oils, as well as the minimum bactericidal concentration and biofilm inhibition activities. Both essential oils Oregano and Cinnamon bark showed potential antibacterial and antibiofilm activity out of 10 essential oils against isolated biofilm forming bacteria from milk samples.