Regulatory sRNAs and Metabolic Adaptation: Unravelling Ciprofloxacin Utilization in Klebsiella sp. SG01

Regulatory sRNAs and Metabolic Adaptation: Unravelling Ciprofloxacin Utilization in Klebsiella sp. SG01 | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 7 April 2025 V1 Latest version Share on Regulatory sRNAs and Metabolic Adaptation: Unravelling Ciprofloxacin Utilization in Klebsiella sp. SG01 Authors : Sriradha Ganguli 0009-0008-7513-9214 and Ranadhir Chakraborty 0000-0002-8299-8358 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174401656.60165496/v1 192 views 109 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Bacterial adaptation to environmental stress is greatly aided by small regulatory RNAs (sRNAs). Here, we examine metabolic adaptation to ciprofloxacin (CIP) stress via sRNA in Klebsiella sp. SG01. We identified intergenic regions enriched with sRNAs and expression patterns described by examining transcriptomic datasets. Nine potential sRNAs, glmZ, sgrS, gcvB, spot42, micA, istR, sraL, icsR, and fnrS were identified by combining differential expression analysis with established computational pipeline. Furthermore, an FMN aptamer (RFN element) was found, indicating a possible function in regulation mediated by riboswitches. These sRNAs target important metabolic pathways, such as the tricarboxylic acid cycle (TCA), redox homeostasis, and the transport of sugars and amino acids, according to functional annotation using TargetRNA3 predictions and literature mining. GcvB was found to negatively regulate ABC transporters and amino acid permeases, whereas Spot42 was found to regulate sugar transporters and TCA intermediates. A dynamic rewiring of the sRNA network under CIP stress was also suggested by transcriptomic analysis, which showed a regulatory shift marked by the downregulation of Hfq and the upregulation of ProQ and CsrA. This study opens the door for further investigation into the complex post-transcriptional regulatory systems that bacteria use to maximize resource allocation and stress resilience. Supplementary Material File (main manuscript.docx) Download 46.81 KB File (table and figures.docx) Download 2.28 MB Information & Authors Information Version history V1 Version 1 07 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords klebsiella antibiotic biodegradation ciprofloxacin srnas transcriptome Authors Affiliations Sriradha Ganguli 0009-0008-7513-9214 University of North Bengal View all articles by this author Ranadhir Chakraborty 0000-0002-8299-8358 [email protected] University of North Bengal View all articles by this author Metrics & Citations Metrics Article Usage 192 views 109 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Sriradha Ganguli, Ranadhir Chakraborty. Regulatory sRNAs and Metabolic Adaptation: Unravelling Ciprofloxacin Utilization in Klebsiella sp. SG01. Authorea . 07 April 2025. DOI: https://doi.org/10.22541/au.174401656.60165496/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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