Partial Least Squares Analysis of the Relationship Among Organic Acid, Total Soluble Solids, Total Phenolic, and Total Flavonoids in Ficus carica

Partial Least Squares Analysis of the Relationship Among Organic Acid, Total Soluble Solids, Total Phenolic, and Total Flavonoids in Ficus carica | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Partial Least Squares Analysis of the Relationship Among Organic Acid, Total Soluble Solids, Total Phenolic, and Total Flavonoids in Ficus carica Jiajie Luo, Xueqian Dong, Jing Liang, Ming Jia, Lei Sun, Yanling Han, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4263609/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Figs ( Ficus carica Linn.) are a new generation of economic forest crops with high nutritional value. This study aimed to clarify the intrinsic association between organic acids and the quality of figs after introducing multispecies. The figs introduced in Shandong are rich in organic acids and contain a wide variety of highly concentrated bioactive compounds. A total of 24 organic acids were detected in this experiment, 13 of which were newly detected. Citric acid was the main organic acid in Shandong-introduced figs, accounting for 66% of the total acid on average. This was followed by malic and citric acids, accounting for 15.9% and 11.6%, respectively. The mean total phenolic, flavonoid, and soluble solid contents were 1.627 mg/g, 0.588 mg/g, and 16.217 °Brix, respectively. The fig cultivar Banane had the highest total organic acid content and high total phenolic and flavonoid contents. The results of the partial least squares regression showed that the regression coefficients of caffeic acid, ferulic acid, chicoric acid, p-hydroxybenzoic acid, and quinic acid on the total soluble solids were in the range of 0.194–0.279, favoring the accumulation of total soluble solids. Trans-aconitic acid, citric acid, cis-aconitic acid, pyruvic acid, and tartaric acid promoted the accumulation of total phenolics and flavonoids. The regression coefficients ranged from 0.200 to 0.310 for total phenolics and 0.233 to 0.290 for total flavonoids. The discovery of a close intrinsic association between organic acids and nutrients provides a new reference basis for screening good fig cultivars and their nutritional development. Ficus carica L. Chemical composition Organic acid Phenolic compounds Flavonoids PLS Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4263609","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":291349468,"identity":"f5d6ea86-f5c5-45ee-b49c-9d8a3f25e88b","order_by":0,"name":"Jiajie Luo","email":"","orcid":"","institution":"Qilu University of Technology (Shandong Academy of Sciences)","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jiajie","middleName":"","lastName":"Luo","suffix":""},{"id":291349469,"identity":"a819d62d-f2d2-4692-ae82-dc7ea03611ec","order_by":1,"name":"Xueqian Dong","email":"","orcid":"","institution":"Qilu University of Technology (Shandong Academy of Sciences)","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Xueqian","middleName":"","lastName":"Dong","suffix":""},{"id":291349472,"identity":"d74bf3b0-f578-43f6-b0be-208b0888f2cc","order_by":2,"name":"Jing Liang","email":"","orcid":"","institution":"Shandong Academy of Forestry Sciences","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jing","middleName":"","lastName":"Liang","suffix":""},{"id":291349475,"identity":"83106478-027e-4c7e-b66f-be2189f73a1b","order_by":3,"name":"Ming Jia","email":"","orcid":"","institution":"Shandong Academy of Forestry Sciences","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ming","middleName":"","lastName":"Jia","suffix":""},{"id":291349476,"identity":"df88de9c-c5c6-45f0-ba85-dbe8b0d05d32","order_by":4,"name":"Lei Sun","email":"","orcid":"","institution":"Shandong Academy of Forestry Sciences","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Lei","middleName":"","lastName":"Sun","suffix":""},{"id":291349484,"identity":"9f9014c3-4131-4649-b7b7-2d5c9abf7b99","order_by":5,"name":"Yanling Han","email":"","orcid":"","institution":"Qilu University of Technology (Shandong Academy of Sciences)","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yanling","middleName":"","lastName":"Han","suffix":""},{"id":291349486,"identity":"19540a86-29e0-40ee-b135-d9d4d0dbcf87","order_by":6,"name":"Rui Sun","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAn0lEQVRIiWNgGAWjYFAC5gMMD0D0AeK1sCUwJJCohceARC3ys898vJHYxiDHdyOB8XMBMVoMzuVutgBqMZa8kcAsPYMoLTy82ySAWhI33EhgY+YhymE9PM9AWuqJ18JwhocNpCXBgGgtBmfYjC0SzkkYzjzzsFmaSIcxP7zxocxGnu948sHPxDkMCCTAiIGxgVgNEPWjYBSMglEwCnADAP35LKbsdB83AAAAAElFTkSuQmCC","orcid":"","institution":"Qilu University of Technology (Shandong Academy of Sciences)","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Rui","middleName":"","lastName":"Sun","suffix":""}],"badges":[],"createdAt":"2024-04-14 04:44:21","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4263609/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4263609/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":55547370,"identity":"f9157a78-dafd-447f-9081-b9841e8fe614","added_by":"auto","created_at":"2024-04-29 19:37:34","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1200328,"visible":true,"origin":"","legend":"","description":"","filename":"PartialLeastSquaresAnalysisoftheRelationshipAmongOrganicAcidTotalSolubleSolidsTotalPhenolicandTotalFlavonoidsinFicuscarica.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4263609/v1_covered_6f2781d9-be9d-441b-9961-6b5d2e9afc2a.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Partial Least Squares Analysis of the Relationship Among Organic Acid, Total Soluble Solids, Total Phenolic, and Total Flavonoids in Ficus carica","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Ficus carica L., Chemical composition, Organic acid, Phenolic compounds, Flavonoids, PLS","lastPublishedDoi":"10.21203/rs.3.rs-4263609/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4263609/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eFigs (\u003cem\u003eFicus carica\u003c/em\u003e Linn.) are a new generation of economic forest crops with high nutritional value. This study aimed to clarify the intrinsic association between organic acids and the quality of figs after introducing multispecies. The figs introduced in Shandong are rich in organic acids and contain a wide variety of highly concentrated bioactive compounds. A total of 24 organic acids were detected in this experiment, 13 of which were newly detected. Citric acid was the main organic acid in Shandong-introduced figs, accounting for 66% of the total acid on average. This was followed by malic and citric acids, accounting for 15.9% and 11.6%, respectively. The mean total phenolic, flavonoid, and soluble solid contents were 1.627 mg/g, 0.588 mg/g, and 16.217 \u0026deg;Brix, respectively. The fig cultivar Banane had the highest total organic acid content and high total phenolic and flavonoid contents. The results of the partial least squares regression showed that the regression coefficients of caffeic acid, ferulic acid, chicoric acid, p-hydroxybenzoic acid, and quinic acid on the total soluble solids were in the range of 0.194\u0026ndash;0.279, favoring the accumulation of total soluble solids. Trans-aconitic acid, citric acid, cis-aconitic acid, pyruvic acid, and tartaric acid promoted the accumulation of total phenolics and flavonoids. The regression coefficients ranged from 0.200 to 0.310 for total phenolics and 0.233 to 0.290 for total flavonoids. The discovery of a close intrinsic association between organic acids and nutrients provides a new reference basis for screening good fig cultivars and their nutritional development.\u003c/p\u003e","manuscriptTitle":"Partial Least Squares Analysis of the Relationship Among Organic Acid, Total Soluble Solids, Total Phenolic, and Total Flavonoids in Ficus carica","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-04-18 02:08:55","doi":"10.21203/rs.3.rs-4263609/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"a15959ad-549d-41a3-9700-0f40c0cab7d3","owner":[],"postedDate":"April 18th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-04-29T19:29:20+00:00","versionOfRecord":[],"versionCreatedAt":"2024-04-18 02:08:55","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4263609","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4263609","identity":"rs-4263609","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}