MiR-130a-5p prevents angiotensin II-induced podocyte apoptosis by modulating M-type phospholipase A2 receptor.

Liu D, Dongwei Liu, Liu F, Fengxun Liu, Wang X, Xutong Wang, Yingjin Qiao, Qiao Y, Pan S, Shaokang Pan, Yang Y, Yang Yang, Hu Y, Yifang Hu, Zhang Y, Yilin Zhang, Fei Tian, Tian F, Liu Z, Zhangsuo Liu
Cell cycle (Georgetown, Tex.) · 2018 · doi:10.1080/15384101.2018.1542901 · PMID:30394845 · PMC6342077
OA: closed
📄 Open PDF View on PubMed View at publisher

Abstract

Podocyte apoptosis is considered as the important element that promotes the development and progress of membranous nephropathy (MN). Unfortunately, the underlying mechanism of podocytes apoptosis in MN remains elusive. We compared the renal expressions of miR-130a-5p and M-type phospholipase A2 receptor (PLA2R) between MN patients (n = 30) and 30 controls by qRT-PCR and western blot, respectively. The podocyte damage model in vitro was established by angiotensin II (Ang II, 100 nmol/L) exposure for 24 h. Interaction between miR-130a-5p and PLA2R was determined using dual-luciferase reporter gene assay. MN mice were induced by intravenous injection of cBSA. In this study, miR-130a-5p expression was significantly decreased both in the renal biopsy specimens from MN patients and podocyte cell line AB8/13 following stimulation of Ang II. Overexpressed miR-130a-5p in AB8/13 cells significantly attenuated the Ang II induced-apoptosis in vitro. In contrast, down-regulated miR-130a-5p induced podocyte apoptosis. PLA2R was identified as the target of miR-130a-5p in AB8/13 cells. And up-regulated or down-regulated PLA2R could obviously attenuate the effect of miR-130a-5p overexpression or knockdown on the apoptosis of AB8/13 cells. Furthermore, it was also observed that overexpressed miR-130a-5p by miR-130a-5p agomir could obviously alleviate renal injury in MN mice. In conclusion, decreased miR-130a-5p was contributed to the pathological mechanism of MN through increasing PLA2R expression, which induced podocyte apoptosis.

My notes (saved in your browser only)

Funding

funders
[{'doi': '10.13039/501100001809', 'name': 'National Natural Science Foundation of China', 'awards': ['U1604284']}]

Citation neighborhood (sparse)

Too few in-corpus citations on either side for a chart; here are the lists.

Cites (1)

References (24)

Source provenance

crossref
last seen: 2026-08-12T06:42:44.631928+00:00
europepmc
last seen: 2026-08-13T06:15:24.848197+00:00
unpaywall
last seen: 2026-08-14T06:25:32.811723+00:00