Physiology-forward identification of bile acid sensitive vomeronasal receptors

Wen Mai Wong, Jie Cao, Xingjian Zhang, Wayne I. Doyle, Luis L. Mercado, Laurent Gautron, Julian P. Meeks
2019 · doi:10.1101/766592
preprint OA: closed CC-BY-NC-4.0
📄 Open PDF View at publisher

Abstract

Abstract/Summary The mouse accessory olfactory system (AOS) supports social and reproductive behavior through the sensation of environmental chemosignals. A growing number of excreted steroids have been shown to be potent AOS cues, including bile acids (BAs) found in feces. As is still the case with most AOS ligands, the specific receptors used by vomeronasal sensory neurons (VSNs) to detect BAs remain unknown. To identify VSN BA receptors, we first performed a deep analysis of VSN BA tuning using volumetric GCaMP6f/s Ca 2+ imaging. These experiments revealed both broadly and narrowly tuned populations of BA-receptive VSNs with sub-micromolar sensitivities. We then developed a new physiology-forward approach for identifying AOS ligand-receptor interactions, which we call F luorescence L ive Imaging for C ell C apture and R NA -seq , or FLICCR-seq. FLICCR-seq analysis revealed 5 specific V1R-family receptors enriched in BA-sensitive VSNs. These studies introduce a powerful new approach for ligand-receptor matching and reveal biological mechanisms underlying mammalian BA chemosensation.

My notes (saved in your browser only)

Citation neighborhood (sparse)

Too few in-corpus citations on either side for a chart; here are the lists.

Cites (1)

References (42)

Source provenance

crossref
last seen: 2026-06-03T07:23:50.635160+00:00
europepmc
last seen: 2026-05-19T01:45:01.086888+00:00
unpaywall
last seen: 2026-05-22T02:00:06.705733+00:00
License: CC-BY-NC-4.0