Aptamer-based diagnosis of various SARS-CoV2 strains isolated from clinical specimens

Zahra Sadat Moshref, Tahmineh Jalali, Razieh Rezaei Adriani, Elahe Soltani, Seyed Latif Mousavi Gargari
2022 · doi:10.21203/rs.3.rs-2006066/v1
preprint OA: gold CC-BY-4.0
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Abstract

The emergence of SARS-CoV2 virus, an unknown strain of coronavirus caused a severe acute respiratory syndrome with high mortality around the world. Late diagnosis of asymptomatic carriers can lead to uncontrollable transmission of the disease. In this study we identified high-binding-affinity aptamers targeting various strains of SARS-CoV2 virus, using GO-SELEX (Graphene Oxide- Systematic Evolution of Ligands by Exponential Enrichment) strategy. A total of 96 aptamers were developed through 11 rounds of GO-SELEX from a random 40 nucleotide ssDNA aptamer library. Using SPR method, the Kd values of all aptamers calculated and two aptamers 52 and 91 with Kd 50 and 61 were selected for ELASA assay. Aptamer 91 could detect various strains of the virus in above 97% of clinical samples obtained from nasopharyngeal swaps (NPS) specimens kept in viral transport media (VTM), confirmed by real-time PCR assay at COVID-19 Reference Diagnostic Laboratory of Iran, Pasture Institute. Aptamer 52 could detect the SARS-CoV2 virus in a competitive LFA assay to be considered for future designed kit. These two simple, specific, and sensitive tests can be used in a combination for rapid and early diagnosis of various strains of COVID19 virus. Our results suggest that these two discovered aptamers present an opportunity for developing new rapid aptamer-based coronavirus diagnostic kit.

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License: CC-BY-4.0