Correlation analysis between drug resistance and virulence genes of carbapenem-resistant Acinetobacter baumannii In Western Guilin

Backgound In order to detect the resistance of Acinetobacter baumannii to β-lactam antibiotics in western Guilin, analyze the reasons for its resistance, and provide laboratory basis for clinical treatment; method for the β-lactamase gene of Acinetobacter baumannii , and to explore the relationship between the presence of the β-lactamase gene of Acinetobacter baumannii and multidrug resistance. Methods From November 2020 to June 2022, 78 non-repeated Acinetobacter baumannii strains from the Second Affiliated Hospital of Guilin Medical University were collected, and their resistance to carbapenem antibiotics was analyzed. PCR was used to detect resistance-related genes ( bla OXA-23 , bla OXA-51 , bla OXA-58 , bla OXA-24 , bla IMP and bla SIM ) and virulence genes ( cusE, abaI and bfmS ). To analyze the relationship between drug resistance and virulence genes of Acinetobacter baumannii . Results Most of the 165 non-enterobacteriaceae bacteria we collected from the hospital were Acinetobacter baumannii and Pseudomonas aeruginosa , and most of the specimen types were from sputum and secretions, and most of them were from respiratory and critical care diseases area and intensive care unit, among them, there are 78 strains of Acinetobacter baumannii studied this time. After screening, 78 Acinetobacter baumannii strains were resistant to Cefazolin, Ampicillin, Amoxicillin/Clavulanate, Aztreonam, Chloramphenicol, Tetracycline, Cefotaxime, Ampicillin/Sulbactam, Piperacillin, Gentamicin, Ciprofloxacin, Levofloxacin, Moxifloxacin, Cefepime, Ceftazidime, Piperacillin/Tazobactam, Compound Sulfonamide, Meropenem, Imipenem, Amikacin, Polymyxin, resistance rates were 98.72%, 98.72%, 96.16%, 96.15%, 96.15%, 66.67%, 60.26%, 58.98%, 58.97%, 57.69%, 57.69%, 57.69%, 57.69%, 57.69%, 56.41%, 55.13%, 53.85%, 52.56%, 52.56%, 50%, 0%. Among them, 17 strains with drug resistance gene bla OXA-51 were detected,accounting for 21.79%; drug gene bla OXA-23 , bla OXA-24 , bla IMP , bla OXA-58 , bla SIM gene were not detected; 17 strains with virulence gene bfmS was detected, accounting for 21.79%; abaI, csuE virulence gene was not detected. Conclusion Acinetobacter baumannii in the hospital is highly resistant to carbapenems, mainly carrying bla OXA-51 resistance genes. Drug resistance is closely related to the virulence gene bfmS .