Lars K. Nielsen

No ORCID on file · 2 papers in corpus

lab-animal 2025

Targeted UDP-glucose ceramide glucosyltransferase stable overexpression induces a metabolic switch improving cell performance at high cell density

Frontiers in bioengineering and biotechnology ·doi:10.3389/fbioe.2025.1690203

BACKGROUND: Viral vectors such as adeno-associated viruses (AAVs) and virus-like particles (VLPs) are critical tools in gene therapy, typically produced using transient gene expression (TGE). Intensification of TGE processes to high cell de…

lab-animal 2022

Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium

·doi:10.22541/au.165606842.29880741/v1

The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibit…