Andrew Woodman

ORCID: 0000-0001-7757-5492 · 1 paper in corpus

2019

RNA-dependent RNA polymerase speed and fidelity are not the only determinants of the mechanism or efficiency of recombination

·doi:10.1101/769224

Using the RNA-dependent RNA polymerase (RdRp) from poliovirus (PV) as our model system, we have shown that Lys-359 in motif-D functions as a general acid in the mechanism of nucleotidyl transfer. A K359H (KH) RdRp derivative is slow and fai…