John J Bromfield

Endometrial inflammation is associated with reduced pregnancy per artificial insemination (AI) and increased pregnancy loss in cows. It was hypothesized that induced endometritis alters histotroph composition and induces inflammatory signat…

BackgroundBacterial infection of the uterus in postpartum dairy cows limits ovarian follicle growth, reduces blood estradiol concentrations, and leads to accumulation of bacterial lipopolysaccharide (LPS) in ovarian follicular fluid. Althou…

Pregnancy induces changes in the transcriptome of the bovine endometrium from 15 days after insemination. However, pregnancy is less likely to occur if cows had a postpartum bacterial infection of the uterus, even after the resolution of di…

score (0.918) for the methylomics dataset. We hope in the future a less invasive biopsy can be used to diagnose endometriosis using the findings from such ensemble machine learning classifiers, as demonstrated in this study.

Endometriosis is a complex and common gynecological disorder yet a poorly understood disease affecting about 176 million women worldwide, and causing significant impact on their quality of life and economic burden. Neither a definitive clin…

Endometriosis is a complex gynecological disorder. The diagnostic process of endometriosis involves an invasive procedure thus delaying the diagnosis for about 10 years on average. Both DNA-methylation data and RNA-seq data has the potentia…

Infections of the reproductive tract or mammary gland with Gram-negative bacteria perturb ovarian function, follicular growth, and fecundity in cattle. We hypothesized that lipopolysaccharide (LPS) from Gram-negative bacteria stimulates an …