Yiwen Li

Background: The global burden of pediatric allergic diseases continues to rise. However, clinical and public health decisions remain guided by an extensive yet fragmented evidence base characterized by substantial uncertainty, hindering th…

Abstract Site-directed mutagenesis and saturation mutagenesis are essential means for gene and protein studies. For plasmid-based gene mutation, PCR and overlap-extension PCR are still the most often used methods; however, the methods usua…