TheStreptococcusphage protein paratox is an intrinsically disordered protein

Iman Asakereh, Nicole R. Rutbeek, Manvir Singh, David Davidson, Gerd Prehna, Mazdak Khajehpour
2024 · doi:10.1101/2024.01.14.575580
preprint OA: closed CC-BY-NC-ND-4.0
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⚙ AI-generated summary by claude@2026-07, 2026-07-14 ⓘ

Streptococcal phage protein paratox is intrinsically disordered in dilute solution but folds upon addition of salts or osmolytes, and adopts a specific globular fold only when bound to its target ComR.

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Abstract

ABSTRACT The bacteriophage protein paratox blocks quorum sensing in its streptococcal host by directly binding the signal receptor and transcription factor ComR. This reduces ability of Streptococcus to uptake environmental DNA and protects phage DNA from damage by recombination. Past work characterizing the paratox:ComR molecular interaction revealed that paratox adopts a well-ordered globular fold when bound to ComR. However, solution-state biophysical measurements suggested that paratox may be conformationally dynamic. To address this discrepancy, we investigated the stability and dynamic properties of paratox in solution using circular dichroism, nuclear magnetic resonance, and several fluorescence-based protein folding assays. Our work shows that under dilute buffer conditions paratox is intrinsically disordered. We also show that the addition of kosmotropic salts or protein stabilizing osmolytes induces paratox folding. However, only the addition of ComR was able to induce paratox to adopt its previously characterized globular fold. Furthermore, as we can induce different paratox folding-states we characterize Prx folding thermodynamics and folding kinetics using stopped flow measurements. Based upon the kinetic results, paratox is a highly dynamic protein in dilute solution, folding and refolding within the 10 ms timescale. Overall, our results demonstrate that the streptococcal phage protein paratox is an intrinsically disordered protein in a two-state equilibrium with a solute-stabilized folded form. Furthermore, the solute-stabilized paratox fold is likely the predominant form of paratox in a solute-crowded bacterial cell. Finally, our work suggests that Prx binds and inhibits ComR, and thus quorum sensing in Streptococcus , by a combination of conformational selection and induced-fit binding mechanisms.

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License: CC-BY-NC-ND-4.0