Methylene Blue Reduces Monoamine Oxidase Expression and Oxidative Stress in Human Cardiovascular Adipose Tissue

Oana-Maria Aburel, Laurențiu Brăescu, Darius G. Buriman, Adrian P. Merce, Anca M. Bînă, Claudia Borza, Cristian Mornoș, Adrian Sturza, Danina M. Muntean
2024 · doi:10.21203/rs.3.rs-4638110/v1
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Abstract

Abstract Cardiovascular diseases represent the major cause of morbidity mainly due to chronic heart failure. Epicardial (EAT) and perivascular adipose tissues (PVAT) are considered major contributors to the pathogenesis of cardiometabolic pathologies. Monoamine oxidases (MAOs) are mitochondrial enzymes recognized as sources of reactive oxygen species (ROS) in cardiometabolic pathologies. Methylene blue (MB) is one of the oldest protective agents, yet no data are available about its effects on adipose tissue. The present pilot study was aimed at assessing the effects of MB: i) on MAO expression and ii) oxidative stress in EAT and PVAT harvested from patients with heart failure subjected to cardiac surgery (n=25). Adipose tissue samples were incubated with MB (0.1 µM/24h) and used for the assessment of MAO gene and protein expression (qPCS and immune fluorescence) and ROS production (confocal microscopy and spectrophotometry). The human cardiovascular adipose tissues contain both MAO isoforms, predominantly MAO-A. Incubation with MB reduced MAOs expression and oxidative stress; co-incubation with serotonin, the MAO-A substrate, further augmented ROS generation, an effect partially reversed by MB. In conclusion, MAO-A is the major isoform expressed in EAT and PVAT and contribute to local oxidative stress; both effects can be mitigated by methylene blue.

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License: CC-BY-4.0