{"paper_id":"ff68236f-ef69-4fe8-9dca-98a6ca6ee30d","body_text":"Acinetobacter lentus sp. nov. isolated from Landfill | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Acinetobacter lentus sp. nov. isolated from Landfill Mengli Xia, Mingxiong He, Yanwei Wang, Bo Wu, Guoquan Hu, Yuandong Zhao This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3910326/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The taxonomic position of strain CAAS 2-6 T , a Gram-negative, aerobic, non-motile, mesophilic bacterium with positive catalase and negative oxidase activity, isolated from Chang'an Landfill in the Longquan Mountains, China. The 16S rRNA gene sequence (1459 bp) of strain CAAS 2-6 T showed high sequence similarity to A. gerneri DSM 14967 T (97.74%), followed by A. kanungonis PS-1 T (97.60%) and A. tandoii DSM 14970 T (97.53%). Phylogenetic analyses based on 16S rRNA, rpoB and gyrB gene sequences, together with DNA–DNA hybridization values less than 70 %, indicated that strain CAAS 2-6 T belongs to the genus Acinetobacter and may represent a novel species. Genome sequencing revealed a genome size of 4.829 Mbp and a G + C content of 57.13 mol%. Major fatty acids identified were Summed Feature 3 (25.78%), C 16:0 (25.74%), C 18:1 ω 9 c (10.46%), C 12:0 (6.96%) and C 16:0 N alcohol (6.45%). The polar lipid profile of strain CAAS 2-6 T revealed the presence of phosphatidylglycerol, phosphatidylcholine, and diphosphatidylglycerol. Based on phenotypic, phenotypic and biochemical characteristics, strain CAAS 2-6 T was clearly distinct from the type strains of other recognized species in the genus Acinetobacter , suggesting that it represents a novel species of the genus Acinetobacter . Therefore, the name Acinetobacter lentus sp. nov. is proposed. The type strain is CAAS 2-6 T (= GDMCC 1.3951 T = KCTC 8156 T = JCM 36321 T ). Figures Figure 1 Figure 2 Figure 3 Introduction The genus Acinetobacter was introduced into taxonomy by Brisou and Prévot (BRISOU and PREVOT 1954 ) and belongs to the family Moraxellaceae , order Moraxellales and class Gammaproteobacteria . Acinetobacter is a taxonomically diverse and ecologically ubiquitous genus of eubacteria that can be found in various environments such as soil (Choi et al. 2013 , Dahal et al. 2017 , Juan et al. 2014 , Kim et al. 2008 , Krizova et al. 2015 , Nemec et al. 2022 ), water ecosystems (Carr et al. 2003 , Li et al. 2014 , Radolfova-Krizova et al. 2016 , Vaz-Moreira et al. 2011 , Y. et al. 2008), plants (Álvarez-Pérez et al. 2013 , Lee et al. 2009 ), animals (Alvarez-Perez et al. 2021 , Carvalheira et al. 2020 , Chen et al. 2022 , Gilroy et al. 2021 , Kim et al. 2014 , Poppel et al. 2016 , Smet et al. 2014 ), and hospital environments (Nemec et al. 2009 , Qin et al. 2020 , Wolf et al. 2021 )(apps.szu.cz/anemec/Classification.pdf). Currently, there are 85 species with validly published names within the genus Acinetobacter ( https://lpsn.dsmz.de/genus/acinetobacter ; last accessed January 3, 2024). The genus is known for its metabolic and physiological diversity (Das et al. 2021 ). Members of Acinetobacter are Gram-negative, aerobic, catalase-positive, oxidase-negative. The G + C content of their genomic DNA ranges from 34.9–47% (Y. et al. 2008). In this study, we present the genomic, chemotaxonomic and phenotypic characteristics of a strain, CAAS 2-6 T , which was isolated from Chang'an Landfill in the Longquan Mountains, China. Those results indicate that strain CAAS 2-6 T represents a new species within the genus Acinetobacter , for which we propose the name Acinetobacter lentus sp. nov. Materials and methods Sample collection and bacterial strains The leachate sample was collected from the Chang'an Landfill site in the Chengdu Longquan Mountains, China(coordinates: 30°65′35.38″N 104°37′81.79″E). To enrich PLA plastic degrading bacteria, 2 g of the collected sample was added to 25 mL of sterile mineral salt medium (MSM). The composition of the MSM was as follows: K 2 HPO 4 7.0 g/L, KH 2 PO 4 2.0 g/L, NH 4 NO 3 1.0 g/L, MgSO 4 ·7H 2 O 0.1 g/L, FeSO 4 ·7H 2 O 0.01 g/L, ZnSO 4 ·7H 2 O 0.001 g/L, MnSO 4 ·6H 2 O 0.0002 g/L and CuSO 4 ·7H 2 O 0.0001 g/L (pH 7.5) (Oshiman et al. 2007 , Vargas-Suárez et al. 2019 , Yang et al. 2015 ). Additionally, 0.02 g of surface sterilized PLA film (≈ 1 × 2 cm 2 ) was added to the medium as the sole carbon source. The PLA films were sterilized by rinsing with 75% ethanol, washing with sterile distilled water, drying in a laminar airflow cabinet, and then exposing to UV irradiation for 10 minutes. The enrichment transfers were conducted by adding 2 mL of the pelagic microbes and PLA film from the previous culture to fresh 25 mL of MSM. This process was repeated every 7 days of incubation at 30°C for a total of five consecutive transfers. After the fifth transfer, the bacteria were isolated and purified. The enrichment culture was spread using the standard dilution-plating technique on Nutrient Broth (NB) agar (Mistry et al. 2022 ). The NB agar medium was prepared by dissolving 10.0 g of peptone, 3.0 g of beef powder, 5.0 g of sodium chloride, and 20 g of agar in one liter of distilled water (pH 7.2). After 72 h of incubation at 30 ℃, individual colonies were randomly selected and repeatedly streaked on the NB agar to obtain pure colonies. The isolate, designated as strain CAAS 2-6 T , was cultured on NB medium for additional taxonomic experiments and preserved as both NB medium slants at 4 ℃ and suspensions with 20% (v/v) glycerol at -80 ℃. Phylogeny For phylogenetic analysis of the 16S rRNA gene, PCR amplification and sequencing were carried out following a previously described method (Xia et al. 2020 ). The obtained 16S rRNA sequence was aligned against the bacterial species recorded in the EzBioCloud database to determine the closest phylogenetic neighbors and their corresponding similarity (Yoon et al. 2017a ). Multiple sequence alignment of 16S rRNA gene sequence of the strain CAAS 2-6 T and its top 32 closest relatives with validly published names was performed by using CLUSTAL X 2.0 software (Larkin et al. 2007 ). The aligned sequences were used to reconstruct the neighbour-joining (NJ) (Saitou and Nei 1987 ) and maximum-likelihood (ML) (Felsenstein 1981 ) phylogenetic trees using the program MEGA 11.0 (Tamura et al. 2021 ), with bootstrap values based on 1000 replications. To further determine the classification position of CAAS 2-6 T within Acinetobacter , comparative sequence analysis of the partial DNA gyrase subunit B (gyrB) gene and RNA polymerase β-subunit (rpoB) genes were performed. These genes are widely used as house-keeping genes for classification and phylogenetic markers of Acinetobacter (Bernard et al. 2006 , Bruno et al. 2014 , Karah et al. 2011 , Nemec et al. 2011 , Nemec et al. 2009 ). Multilocus sequence analysis (MLSA) of concatenated housekeeping gene sequences has proven to be a valuable method for classifying genera and species within the family Acinetobacter (Bruno et al. 2014 , Karah et al. 2011 ). Genome analysis The draft genome of strain CAAS 2-6 T was obtained using the Whole Genome Shotgun (WGS) strategy, which involved constructing libraries with different insertion fragments and performing paired-end (PE) sequencing on the Illumina NovaSeq platform using Next Generation Sequencing (NGS) technology. For high-throughput sequencing, a TruSeqTM DNA Sample Prep Kit was used to construct DNA sequencing libraries following the standard Illumina TruSeq Nano DNA LT library preparation process (Illumina TruSeq DNA Sample Preparation Guide). Subsequently, the paired-end sequencing libraries (2×150 bp) were sequenced using the Illumina NovaSeq platform. Reads were processed and assembled de novo using A5-MiSeq (Coil et al. 2015 ) version 20160825 and SPAdes (Bankevich et al. 2012 ) v3.12.0 ( http://cab.spbu.ru/files/release3.12.0/manual.html ). Based on the results of A5-MiSeq, base correction was performed using Pilon (Walker et al. 2014 ) software v1.18. Open reading frames, rRNA, tRNA and CRISPRs were predicted and annotated using the GeneMarkS v4.32, Barrnap v0.9, tRNAscan-SE v1.3.1 and CRISPRCasFinder v4.2.20 (Besemer et al. 2001 , Couvin et al. 2018 , Kalvari et al. 2018 , Lowe and Eddy 1997 ). The protein sequences encoded by the gene were compared with the protein sequences in the NCBI NR, eggNOG (evolutionary genealogy of genes: Non-supervised Orthologous Groups), KEGG (Kyoto Encyclopedia of Genes and Genomes), Swiss-Prot and GO (Gene Ontology) databases using diamond blastp, and the cut-off value of sequence alignment was selected as 1e-6, and the best Hits were selected for function discrimination. To further confirm that strain CAAS 2-6 T was a novel species of the genus Acinetobacter , genome sequencing of the strain was performed, and in silico DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) tests were conducted between strain CAAS 2-6 T and the closest related type species. Silico ANI and DDH values were calculated using the EzBioCloud web server ( https://www.ezbiocloud.net/tools/ani)(Yoon et al. 2017b) with the recommended algorithm, and the Genome-to-Genome Distance Calculator version 3.0 was used with the recommended Formula 2 ( http://ggdc.dsmz.de ) (Meier-Kolthoff et al. 2013 ). Phenotypic and chemotaxonomic characterization Growth tests were performed at 30°C for 7 days on various media, including Brain-Heart Infusion (BHI) agar, R2A agar, tryptic soy agar (TSA), Luria–Bertani (LB) agar and NB agar. Colony morphology was observed after 48 hours growth on NA plate at 37 ℃. Cell motility was assessed through motility assays in semi-solid medium(including 0.5% agar) and the hanging drop method. Gram staining was performed by using commercial kits as per according to the manufacturer’s guidelines. Cell morphology were observed using scanning electron microscopy (SEM, SU8010, HITACH) after incubation in NB culture to mid-exponential phase at 30 ℃. Growth at different temperature (4, 8, 16, 20, 25, 30, 37, 40, 45, 50 and 60 ℃) in NB medium and at various pH values (pH 4.0–11.0, prepared with appropriate biological buffers at intervals of 0.5 unit) in NB medium at 30 ℃ were determined after 48 hours of incubation. Salt tolerance was determined after 48 h of incubation in NB medium supplemented with 0–15% (w/v) NaCl (at 1.5% intervals). All physiological tests were carried out in triplicates, and growth was measured turbidometrically (OD) at 600 nm using a spectrophotometer and cuvette of 1.0 cm path length. Catalase activity was determined by the observation of the bubble production in a 3% (v/v) hydrogen peroxide solution, while oxidase activity was examined using 1% (w/v) tetramethyl-ρ-phenylenediamine. Acid production from carbohydrates were examined with the API 50CH system (bioMérieux), while other biochemical and enzymatic tests were performed with the API 20NE system (bioMérieux) and API ZYM system (bioMérieux) following the manufacturer’s instructions. Anaerobic growth was assessed by incubating inoculated NB agar plates in Mitsubishi™ AnaeroPouch-Bag at 30℃ for 7 days. Duplicate tests for antibiotic susceptibility of strain CAAS 2-6 T were conducted using the disc diffusion method (Bauer et al. 1966 ) on NB agar at 30℃ with filter paper discs containing various antibiotics and respective concentrations: carbenicillin (100 µg), ampicillin (10 µg), kanamycin (30 µg), cefatriaxone (30 µg), cefoperazone (75 µg), ceftazidime (30 µg), cefazolin (30 µg), gentamicin (10 µg), penicillin (10 U), cefuroxime (30 µg), neomycin (30 µg), cephalexin (30 µg), doxycycline (30 µg), tetracycline (30 µg), piperacillin (100 µg), topaminokana (30 µg), erythromycin (15 µg), cefradine (30 µg), minocycline (30 µg), oxacillin(1 µg), chloramphenicol (20 µg), spectacomycin (200 µg), gentamicin (200 µg). For the determination of the whole-cell fatty acid composition, strain CAAS 2-6 T was cultivated on NB agar at 30 ℃ for 48 hours. The fatty acids were then extracted and analyzed according to the recommendations of the Microbial Identification System (MIDI), a commercial identification system. The whole fatty acid composition was determined using an Agilent 6890N gas chromatograph. For the analysis of lipids, cells were cultured in NB culture until the mid-exponential phase on a rotary shaker at 30 ℃. Polar lipids were extracted from 200 mg of dry cells as described by Bligh and Dyer (BLIGH and DYER 1959 ), followed by separation using two-dimensional thin-layer chromatography (TLC) on silica gel GF254 plates (100×100 mm; haiyangchem). The solvent system used for separation has been previously described (Tindall et al. 2007 ). For the determination of the whole-cell fatty acid composition, strain CAAS 2-6 T was cultivated on NB agar at 30 ℃ for 48 hours. The fatty acids were then extracted and analyzed according to the recommendations of the Microbial Identification System (MIDI), a commercial identification system. The whole fatty acid composition was determined using an Agilent 6890N gas chromatograph. For the analysis of lipids, cells were cultured in NB culture until the mid-exponential phase on a rotary shaker at 30 ℃. Polar lipids were extracted from 200 mg of dry cells as described by Bligh and Dyer (BLIGH and DYER 1959 ), followed by separation using two-dimensional thin-layer chromatography (TLC) on silica gel GF254 plates (100×100 mm; haiyangchem). The solvent system used for separation has been previously described (Tindall et al. 2007 ). Results and Discussion Phylogeny analysis The full-length sequence of 16S rRNA gene (1459 bp) extracted from the draft genome of strain CAAS 2-6 T showed the highest similarity with A. gerneri DSM 14967 T (97.74%), followed by A. kanungonis PS-1 T (97.60%) and A. tandoii DSM 14970 T (97.53%). The ML phylogenetic tree based on 16S rRNA gene sequences (Fig. 1 ) showed that strain CAAS 2-6 T clustered with related species from the genus Acinetobacter . The consistent clustering pattern could also be observed in the NJ phylogenetic tree (Fig. S1 ). It was obvious that strain CAAS 2-6 T belongs to the genus Acinetobacter . The MLSA-derived ML phylogenetic tree (Fig. 2 ) demonstrates that strain CAAS 2-6T forms a separate subclade when compared to the 53 other strains of the genus Acinetobacter . The similar clustering pattern is observed in the MLSA-derived NJ phylogenetic tree (Fig. S2 ). Therefore, our MLSA results suggested that strain CAAS 2-6 T is a member of the genus Acinetobacter close to A. radioresistens , but representing a distinct species. Genome features A total of 1.235 Gb of raw DNA sequence was generated, which was assembled into 73 scaffolds and 469× coverage. The assembled draft genome of strain CAAS 2-6 T was estimated to be at least 2,593,745 bp in size with a G + C content of 44.41%. In total, 2,344 genes, 59 tRNA, 3 rRNA and 0 CRISPRs were predicted in the assembled draft genome. NR annotations of protein-coding genes are shown in Table S1 . The four databases (NR, eggNOG, KEGG, and Swiss-Prot) annotated 2,344, 2,089, 1,783, and 1,803 gene functions, respectively, and these functional genes were classified and statistically displayed in Fig. 3 . The genome sequence of CAAS 2-6 T has been deposited in the DDBJ/ ENA/GenBank under accession No. JAQIHG000000000. Genome sequences of closely related taxa were obtained from the NCBI GenBank database. In silico DDH tests revealed that strain CAAS 2-6 T had the highest genomes similarity of 22.7% with A. indicus CIP 110367 T . The DNA relatedness values of CAAS 2-6 T with other Acinetobacter species ranged from 19.7 to 22.3% (Table S2 ). Furthermore, strain CAAS 2-6 T showed the highest genome similarity of 77.00% with A. indicus CIP 110367 T . and the ANI values between strain CAAS 2-6 T and other Acinetobacter species ranged from 74.49 to 76.59% (Table S2 ). The DDH values and ANI values between strain CAAS 2-6 T and other type species in the genus Acinetobacter were lower than the generally accepted species-level boundary of 70% and 95 − 6%, respectively (Chun et al. 2018 ). Based on these findings, it can be concluded that strain CAAS 2-6 T represents a previously unidentified species within the genus Acinetobacter . Phenotypic and Chemotaxonomic characterization Colonies of strain CAAS 2-6 T on NB agar were circular, smooth and 0.8–1.2 mm in diameter. Strain CAAS 2-6 T was Gram-staining-negative, aerobic, and short rod shaped, with a size of approximately 0.7 µm×0.9–1.6 µm (Fig. S3). Strain CAAS 2-6 T was able to grow at 15–40 ℃ (optimally at 25–40 ℃), at pH 6.0–8.0 (optimally at pH 7.0–7.5) and in the presence of 0–6% (w/v) NaCl (optimally at 0–4%). Strain CAAS 2-6 T exhibited resistance to Penicillin, Ceftazidime, and Oxacillin(= 0 cm), showed weak resistance to Ampicillin, Cefazolin, Gentamicin, Neomycin, Cephalexin, and Cefradine(≤ 1.5 cm), while being susceptible to the remaining 14 antibiotics. Differential phenotypic characteristics between strain CAAS 2-6 T and its closer affinity species of the genus Acinetobacter were summarized in Table 1 . The API ZYM, API 20NE and API 50CH results confirmed that the classification of strain CAAS 2-6 T as a member of the genus Acinetobacter . For example, all known species within the genus Acinetobacter lack the ability to produce arginine hydrolase, chymotrypsin α-galactosidase, β-galactosidase, α-glucosidase, β-glucosidase, β-glucuronidase, N-acetyl-β-glucosaminase, α-mannosidase, and β-fucosidase. Conversely, they exhibit the ability to produce esterase and leucine-arylamidase, lipase, valine arylamidase and cystinol-arylamidase. However, strain CAAS 2-6 T differed from all recognized species of genus Acinetobacter . It could be distinguishable from other species in genus Acinetobacter by the ability to ferment glucose, as well as the absence of acid phosphomonoesterase activity (Table S3). The whole-cell fatty acid profiles of strain CAAS 2-6 T and other species in the genus Acinetobacter are presented in Table 2. Strain CAAS 2-6 T exhibited the similarly predominant fatty acids, including C 12:0 (7.0%), C 16:0 (25.7%), C 16:0 N alcohol(6.5%), C 18:1 ω 9 c (10.5%) and Summed Feature 3(25.8%). The detailed fatty acid profile of strain CAAS 2-6 T can be found in Table 1 . The polar lipid composition mainly consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylcholine(PC) (Fig. S4). These profiles were similar to those of recently described strains wihin the genus Acinetobacter (Das et al. 2021 , Feng et al. 2014 , Malhotra et al. 2012 ). The analysis of chemotaxonomic properties, including fatty acid profiles and polar lipid composition, confirmed the placement of strain CAAS 2-6 T within the genus Acinetobacter as a novel species. Table 1 Fatty acid composition of strain CAAS 2-6 T and its phylogenetically closest relatives Strains: 1, CAAS 2-6 T ; 2, A. chinensis WCHAc010005 T ; 3, A. sichuanensis WCHAc060041 T ; 4, A. chengduensis WCHAc060005 T ; 5, A. wuhouensis WCHA60 T . All dates from this study. –, Not detected. Fatty acids present at > 5 % are highlighted in bold. Summed Feature 2 comprised C 12:0 aldehyde ?, Summed Feature 3 comprised C 16:1 ω 7 c and/or C 16:1 ω 6 c , Summed Feature 5 comprised C 18:2 ω 6, 9 c and/or Ante-C 18:0 , Summed Feature 8 comprised C 18:1 ω 7 c . Fatty acid 1 2 3 4 5 C 10:0 0.22 0.55 0.19 1.20 0.34 C 12:0 6.96 12.06 5.61 13.60 13.68 Iso-C 13:0 1.82 – – 3.17 – C 12:0 2-OH 0.51 1.75 – 2.05 – C 12:0 3-OH 3.37 7.94 – 7.04 5.87 Iso-C 14:0 1.29 – – 1.90 0.72 C 14:0 1.20 0.50 9.78 1.72 0.96 Iso-C 15:0 3.84 – – 1.98 0.65 Anteiso-C 15:0 1.32 – – 1.45 6.33 C 16:1 ω 7 c alcohol 1.77 – – – – C 16:0 N alcohol 6.45 – – – – Iso-C 16:0 1.55 – – 0.44 4.68 C 16:0 25.74 17.90 25.60 19.17 14.46 Iso-C 17:0 0.10 1.48 0.29 0.83 0.82 Iso-C 17:1 ω 10 c 1.06 – 9.13 – – C 17:0 0.19 – 0.83 0.94 1.18 C 18:3 ω 6 c 2.08 – – – – C 18:1 ω 9 c 10.46 24.31 – 19.84 18.18 C 18:0 1.06 0.90 1.53 2.44 2.86 C 19:0 cyclo ω 8 c – – 1.36 – – Summed Feature 2 0.54 – 12.79 – 0.54 Summed Feature 3 25.78 27.47 10.76 20.40 25.82 Summed Feature 5 – – – – 0.22 Summed Feature 8 0.60 4.50 21.78 – 2.50 Description of A cinetobacter lentus sp. nov. Acinetobacter lentus (len’tus. L. masc. adj. lentus, slow, delayed, referring to slow growth). Cells are Gram-staining-negative, aerobic, non-motile, short rods (approx. 0.7 µm×0.9–1.6 µm). Growth occurs on BHI agar, R2A agar, TSA, NA and LB agar. Colonies grown on NB agar medium are circular, smooth, and 0.8–1.2 mm in diameter within 24 hours at 37 ℃. Growth occurs at 15–40 ℃ (optimally at 25–40 ℃), at pH 6.0–8.0 (optimally at pH 7.0–7.5) and in the presence of 0–6% (w/v) NaCl (optimally at 0–4%). Positive results were obtained for catalase, alkaline phosphomonoesterase, esterase(C4 and C8), leucine-arylamidase, valine arylamidase and Naphthol-AS-BI-phosphoric acid, negative results were observed for oxidase, arginine dihydrolase, urease, gelatinase, lipase(C14), cystinol- arylamidase, trypsin, chymotrypsin, acid phosphomonoesterase, α-galactosidase, β-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl-β-glucosaminase, α-mannosidase and β-fucosidase. In the API 20NE system, assimilation occurred on L-arabinose, D-mannose, N-acetyl glucosamine, D-maltose, gluconate, decanoic acid, malic acid, citric acid and phenylacetic acid. In the API 50CH system, acids are produced from glucose, furctoseribose, N-acetylglucosamine, esculin, maltose, starch; are weakly produced from arbutin; and are not produced from glycerinum, erythrose, D-arabinose, L-arabinose, ribose, D-xylose, L-xylose, adonitol, β-methyl-D-xyloside, galactose, mannose, sorbose, rhamnose, dulcitol, inositol, mannitol, sorbitol, α-metyl-D-mannosidase, α-metyl-D-glucoside, amygdalin, salicoside, cellobiose, lactose, melibiose, sucrose, trehalose, inulin, melezitose, raffinose, glycogen, xylitol, geranyl, D-turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, gluconat, 2-keto-gluconate, 5-keto-gluconate. The predominant fatty acids were identified as C 12:0 , C 16:0 N alcohol, C 16:0 , C 18:1 ω 6 c , and Summed Feature 3. Polar lipids include DPG, PG, and PC. The type strain, CAAS 2-6 T (= GDMCC 1.3951 T = KCTC 8156 T = JCM 36321 T ), was isolated from Chang'an Landfill in the Longquan Mountains, China. The DNA G + C content of the type strain is 44.41 mol%. The GenBank accession numbers for the draft genome assembly and 16S rRNA gene sequences of strain Acinetobacter lentus CAAS 2-6 T are JAQIHG000000000 and OQ110573 respectively. Abbreviations ANI average nucleotide identity BHI Brain-Heart Infusion CRISPRs Clustered Regularly Interspaced Short Palindromic Repeats dDDH digital DNA–DNA hybridization MSM mineral salt medium GO Gene Ontology GDMCC Guangdong Microbial Culture Collection Center JCM Japan Collection of Microorganisms KCTC Korean Collection for Type Cultures KEGG Kyoto Encyclopedia of Genes and Genomes MEGA Molecular Evolutionary Genetics Analysis LB Luria-Bertani NB Nutrient Broth ML maximum-likelihood NJ neighbor-joining MIDI Microbial Identification System DPG diphosphatidylglycerol PG phosphatidylglycerol PC phosphatidylcholine TLC Thin layer chromatography TSA tryptic soy agar. Declarations We sincerely appreciate the help of Associate Research Shichun Ma's team from the Biogas Science Research Institute of the Chinese Academy of Agricultural Sciences in using the MIDI system for fatty acids composition determination and polar lipids identification. Conflicts of interest The authors have no relevant financial or non-financial interests to disclose. Funding information This work was financially supported by National Natural Science Foundation of China (No. 31600012), Sichuan science and technology program (2018JY0216, 2022YFS0508), Qinghai Science and Technology program (2022-NK-124) and chengdu science and technology program (2022-YF05-00753). Author Contribution All authors contributed to the study conception and design. Mengli Xia performed the experiment and writing - original draft, Yuandong Zhao performed the experiment, Bo Wu contributed significantly to analysis and manuscript preparation, Guoquan Hu performed the data analyses, Mingxiong He conceptualization and supervision, Yanwei Wang conceptualization and supervision. All authors read and approved the final manuscript. 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Walker, B.J., Abeel, T., Shea, T., Priest, M., Abouelliel, A., Sakthikumar, S., Cuomo, C.A., Zeng, Q., Wortman, J., Young, S.K. and Earl, A.M. (2014) Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement. 9(11), e112963. Wolf, S., Barth-Jakschic, E., Birkle, K., Bader, B., Marschal, M., Liese, J., Peter, S. and Oberhettinger, P. (2021) Acinetobacter geminorum sp. nov., isolated from human throat swabs. 71(10). Xia, M., Wang, J., Huo, Y.-X. and Yang, Y. (2020) Mixta tenebrionis sp. nov., isolated from the gut of the plastic-eating mealworm Tenebrio molitor L. 70(2), 790–796. Y., P.A., Harald, S. and L., P.D. (2008) Acinetobacter baumannii: Emergence of a Successful Pathogen. 21(3), 538–582. Yang, Y., Yang, J., Wu, W.-M., Zhao, J., Song, Y., Gao, L., Yang, R. and Jiang, L. (2015) Biodegradation and Mineralization of Polystyrene by Plastic-Eating Mealworms: Part 2. Role of Gut Microorganisms. 49(20), 12087–12093. Yoon, S.-H., Ha, S.-M., Kwon, S., Lim, J., Kim, Y., Seo, H. and Chun, J. (2017a) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. 67(5), 1613–1617. Yoon, S.-H., Ha, S.-m., Lim, J., Kwon, S. and Chun, J. (2017b) A large-scale evaluation of algorithms to calculate average nucleotide identity. 110(10), 1281–1286. Additional Declarations No competing interests reported. Supplementary Files SupplementaryInformation.docx SupplementaryInformation.xls Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {\"props\":{\"pageProps\":{\"initialData\":{\"identity\":\"rs-3910326\",\"acceptedTermsAndConditions\":true,\"allowDirectSubmit\":true,\"archivedVersions\":[],\"articleType\":\"Research Article\",\"associatedPublications\":[],\"authors\":[{\"id\":270407454,\"identity\":\"431fc048-e080-446f-8c25-47d7fd05f872\",\"order_by\":0,\"name\":\"Mengli Xia\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Biogas Institute of Ministry of Agriculture\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Mengli\",\"middleName\":\"\",\"lastName\":\"Xia\",\"suffix\":\"\"},{\"id\":270407455,\"identity\":\"da29b75d-073b-4dd1-8b35-05eabdd224a4\",\"order_by\":1,\"name\":\"Mingxiong He\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Biogas Institute of Ministry of Agriculture\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Mingxiong\",\"middleName\":\"\",\"lastName\":\"He\",\"suffix\":\"\"},{\"id\":270407456,\"identity\":\"33b0a181-5c0a-4cd4-884f-de6f8538f00d\",\"order_by\":2,\"name\":\"Yanwei Wang\",\"email\":\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA30lEQVRIiWNgGAWjYJCCAx8qbHjY2BsSHyRU1BClg/HgjDNpMnw8Bx4bPDhzjCgtzId52w7ZyEkkPpN82MJMWL3BjRyDwzxnDvCwMSSnVSQ2sDHwt3cnENRycE7FHaCWY2k3EnfIMEicObsBrxazG7kbDrw584yHjbEHqOUMG4OBRC4RWnjbDvOwMfN/K0hsYyZOy0GwFjaGNAaitNifef8BFMg8bDwMyRIJZ47xEPSLZHta8gdgVNrLz3+Q+PFHRY0cf3svfi0MAgmofB78ykGA/wBhNaNgFIyCUTDCAQCRPlOSd9LIGQAAAABJRU5ErkJggg==\",\"orcid\":\"\",\"institution\":\"Biogas Institute of Ministry of Agriculture\",\"correspondingAuthor\":true,\"prefix\":\"\",\"firstName\":\"Yanwei\",\"middleName\":\"\",\"lastName\":\"Wang\",\"suffix\":\"\"},{\"id\":270407457,\"identity\":\"779d1174-907c-4347-a0e3-62c2633d39ec\",\"order_by\":3,\"name\":\"Bo Wu\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Biogas Institute of Ministry of Agriculture\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Bo\",\"middleName\":\"\",\"lastName\":\"Wu\",\"suffix\":\"\"},{\"id\":270407458,\"identity\":\"2bcf52e5-7add-4d7c-a880-af8c1ff4e23b\",\"order_by\":4,\"name\":\"Guoquan Hu\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Biogas Institute of Ministry of Agriculture\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Guoquan\",\"middleName\":\"\",\"lastName\":\"Hu\",\"suffix\":\"\"},{\"id\":270407459,\"identity\":\"b8ae0a92-ed90-4951-bb23-7be439776adc\",\"order_by\":5,\"name\":\"Yuandong Zhao\",\"email\":\"\",\"orcid\":\"\",\"institution\":\"Biogas Institute of Ministry of Agriculture\",\"correspondingAuthor\":false,\"prefix\":\"\",\"firstName\":\"Yuandong\",\"middleName\":\"\",\"lastName\":\"Zhao\",\"suffix\":\"\"}],\"badges\":[],\"createdAt\":\"2024-01-30 10:49:40\",\"currentVersionCode\":1,\"declarations\":\"\",\"doi\":\"10.21203/rs.3.rs-3910326/v1\",\"doiUrl\":\"https://doi.org/10.21203/rs.3.rs-3910326/v1\",\"draftVersion\":[],\"editorialEvents\":[],\"editorialNote\":\"\",\"failedWorkflow\":false,\"files\":[{\"id\":50485355,\"identity\":\"c9824d59-6b9d-429f-bd13-3f9965bdbf58\",\"added_by\":\"auto\",\"created_at\":\"2024-02-01 09:00:55\",\"extension\":\"png\",\"order_by\":1,\"title\":\"Figure 1\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":144197,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eMaximum-likelihood tree based on 16S rRNA gene sequences of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and its top 32 closest relatives with validly published names. Bootstrap values based on 1000 replicates are expressed as percentages. The 16S rRNA gene sequence of \\u003cem\\u003eMoraxella lacunata \\u003c/em\\u003eNBRC 102154\\u003csup\\u003eT\\u003c/sup\\u003e was used as an outgroup. Bar represents 0.01 substitutions per nucleotide position. Bootstrap values based on 1000 replicates are expressed as percentages. Less than 70 of the Bootstrap value are not displayed.\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"figure1.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-3910326/v1/1122a3525cdb2a1dd9b1b187.png\"},{\"id\":50485356,\"identity\":\"148f9dc4-6867-46d8-b120-fb4f47d76234\",\"added_by\":\"auto\",\"created_at\":\"2024-02-01 09:00:55\",\"extension\":\"png\",\"order_by\":2,\"title\":\"Figure 2\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":261096,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eMaximum-likelihood tree based on concatenated partial nucleotide sequences of the gyrB (664 bp) and rpoB (742 bp) genes of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and recognized species/the type or reference strains of known species or taxa of the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e. Similar protein sequences of \\u003cem\\u003eMoraxella lacunata \\u003c/em\\u003eNBRC 102154\\u003csup\\u003eT\\u003c/sup\\u003e were used as an outgroup. Bootstrap values based on 1000 replicates are expressed as percentages. Less than 50 of the Bootstrap value are not displayed.\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"figure2.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-3910326/v1/864637baad1bba0a7533f77f.png\"},{\"id\":50485357,\"identity\":\"a7ec70bf-804c-4148-8e05-6aaf0cf88bdc\",\"added_by\":\"auto\",\"created_at\":\"2024-02-01 09:00:55\",\"extension\":\"png\",\"order_by\":3,\"title\":\"Figure 3\",\"display\":\"\",\"copyAsset\":false,\"role\":\"figure\",\"size\":435217,\"visible\":true,\"origin\":\"\",\"legend\":\"\\u003cp\\u003eAnnotated classification of protein-coding genes. (a) eggNOG, (b) KEGG, (c) GO.\\u003c/p\\u003e\",\"description\":\"\",\"filename\":\"3.png\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-3910326/v1/ccd20c833f144fda93c2a5fe.png\"},{\"id\":60916298,\"identity\":\"0e58c08e-2897-4fb5-936b-bcbe1664c6d1\",\"added_by\":\"auto\",\"created_at\":\"2024-07-23 13:46:15\",\"extension\":\"pdf\",\"order_by\":0,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"manuscript-pdf\",\"size\":1500148,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"manuscript.pdf\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-3910326/v1/c30c2822-2b74-4972-9f24-adf6cb7fd630.pdf\"},{\"id\":50485358,\"identity\":\"d0d3c89f-a44c-4839-8840-a9375611aa76\",\"added_by\":\"auto\",\"created_at\":\"2024-02-01 09:00:55\",\"extension\":\"docx\",\"order_by\":12,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"supplement\",\"size\":2269730,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"SupplementaryInformation.docx\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-3910326/v1/f9823a2f952a3dd0b2479319.docx\"},{\"id\":50485359,\"identity\":\"0ec79859-75c3-4967-a59e-7b17bc2c2c35\",\"added_by\":\"auto\",\"created_at\":\"2024-02-01 09:00:55\",\"extension\":\"xls\",\"order_by\":13,\"title\":\"\",\"display\":\"\",\"copyAsset\":false,\"role\":\"supplement\",\"size\":710836,\"visible\":true,\"origin\":\"\",\"legend\":\"\",\"description\":\"\",\"filename\":\"SupplementaryInformation.xls\",\"url\":\"https://assets-eu.researchsquare.com/files/rs-3910326/v1/bdef8a711bb0614902cdd7f7.xls\"}],\"financialInterests\":\"No competing interests reported.\",\"formattedTitle\":\"Acinetobacter lentus sp. nov. isolated from Landfill\",\"fulltext\":[{\"header\":\"Introduction\",\"content\":\"\\u003cp\\u003eThe genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e was introduced into taxonomy by Brisou and Pr\\u0026eacute;vot (BRISOU and PREVOT \\u003cspan citationid=\\\"CR8\\\" class=\\\"CitationRef\\\"\\u003e1954\\u003c/span\\u003e) and belongs to the family \\u003cem\\u003eMoraxellaceae\\u003c/em\\u003e, order \\u003cem\\u003eMoraxellales\\u003c/em\\u003e and class \\u003cem\\u003eGammaproteobacteria\\u003c/em\\u003e. \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e is a taxonomically diverse and ecologically ubiquitous genus of eubacteria that can be found in various environments such as soil (Choi et al. \\u003cspan citationid=\\\"CR13\\\" class=\\\"CitationRef\\\"\\u003e2013\\u003c/span\\u003e, Dahal et al. \\u003cspan citationid=\\\"CR17\\\" class=\\\"CitationRef\\\"\\u003e2017\\u003c/span\\u003e, Juan et al. \\u003cspan citationid=\\\"CR22\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e, Kim et al. \\u003cspan citationid=\\\"CR25\\\" class=\\\"CitationRef\\\"\\u003e2008\\u003c/span\\u003e, Krizova et al. \\u003cspan citationid=\\\"CR27\\\" class=\\\"CitationRef\\\"\\u003e2015\\u003c/span\\u003e, Nemec et al. \\u003cspan citationid=\\\"CR37\\\" class=\\\"CitationRef\\\"\\u003e2022\\u003c/span\\u003e), water ecosystems (Carr et al. \\u003cspan citationid=\\\"CR10\\\" class=\\\"CitationRef\\\"\\u003e2003\\u003c/span\\u003e, Li et al. \\u003cspan citationid=\\\"CR30\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e, Radolfova-Krizova et al. \\u003cspan citationid=\\\"CR41\\\" class=\\\"CitationRef\\\"\\u003e2016\\u003c/span\\u003e, Vaz-Moreira et al. \\u003cspan citationid=\\\"CR47\\\" class=\\\"CitationRef\\\"\\u003e2011\\u003c/span\\u003e, Y. et al. 2008), plants (\\u0026Aacute;lvarez-P\\u0026eacute;rez et al. \\u003cspan citationid=\\\"CR2\\\" class=\\\"CitationRef\\\"\\u003e2013\\u003c/span\\u003e, Lee et al. \\u003cspan citationid=\\\"CR29\\\" class=\\\"CitationRef\\\"\\u003e2009\\u003c/span\\u003e), animals (Alvarez-Perez et al. \\u003cspan citationid=\\\"CR1\\\" class=\\\"CitationRef\\\"\\u003e2021\\u003c/span\\u003e, Carvalheira et al. \\u003cspan citationid=\\\"CR11\\\" class=\\\"CitationRef\\\"\\u003e2020\\u003c/span\\u003e, Chen et al. \\u003cspan citationid=\\\"CR12\\\" class=\\\"CitationRef\\\"\\u003e2022\\u003c/span\\u003e, Gilroy et al. \\u003cspan citationid=\\\"CR21\\\" class=\\\"CitationRef\\\"\\u003e2021\\u003c/span\\u003e, Kim et al. \\u003cspan citationid=\\\"CR26\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e, Poppel et al. \\u003cspan citationid=\\\"CR39\\\" class=\\\"CitationRef\\\"\\u003e2016\\u003c/span\\u003e, Smet et al. \\u003cspan citationid=\\\"CR43\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e), and hospital environments (Nemec et al. \\u003cspan citationid=\\\"CR36\\\" class=\\\"CitationRef\\\"\\u003e2009\\u003c/span\\u003e, Qin et al. \\u003cspan citationid=\\\"CR40\\\" class=\\\"CitationRef\\\"\\u003e2020\\u003c/span\\u003e, Wolf et al. \\u003cspan citationid=\\\"CR49\\\" class=\\\"CitationRef\\\"\\u003e2021\\u003c/span\\u003e)(apps.szu.cz/anemec/Classification.pdf). Currently, there are 85 species with validly published names within the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e (\\u003cspan class=\\\"ExternalRef\\\"\\u003e\\u003cspan class=\\\"RefSource\\\"\\u003ehttps://lpsn.dsmz.de/genus/acinetobacter\\u003c/span\\u003e\\u003cspan address=\\\"https://lpsn.dsmz.de/genus/acinetobacter\\\" targettype=\\\"URL\\\" class=\\\"RefTarget\\\"\\u003e\\u003c/span\\u003e\\u003c/span\\u003e; last accessed January 3, 2024). The genus is known for its metabolic and physiological diversity (Das et al. \\u003cspan citationid=\\\"CR18\\\" class=\\\"CitationRef\\\"\\u003e2021\\u003c/span\\u003e). Members of \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e are Gram-negative, aerobic, catalase-positive, oxidase-negative. The G\\u0026thinsp;+\\u0026thinsp;C content of their genomic DNA ranges from 34.9\\u0026ndash;47% (Y. et al. 2008). In this study, we present the genomic, chemotaxonomic and phenotypic characteristics of a strain, CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e, which was isolated from Chang'an Landfill in the Longquan Mountains, China. Those results indicate that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e represents a new species within the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e, for which we propose the name \\u003cem\\u003eAcinetobacter lentus\\u003c/em\\u003e sp. nov.\\u003c/p\\u003e\"},{\"header\":\"Materials and methods\",\"content\":\"\\u003cdiv id=\\\"Sec3\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003eSample collection and bacterial strains\\u003c/h2\\u003e \\u003cp\\u003eThe leachate sample was collected from the Chang'an Landfill site in the Chengdu Longquan Mountains, China(coordinates: 30\\u0026deg;65\\u0026prime;35.38\\u0026Prime;N 104\\u0026deg;37\\u0026prime;81.79\\u0026Prime;E). To enrich PLA plastic degrading bacteria, 2 g of the collected sample was added to 25 mL of sterile mineral salt medium (MSM). The composition of the MSM was as follows: K\\u003csub\\u003e2\\u003c/sub\\u003eHPO\\u003csub\\u003e4\\u003c/sub\\u003e 7.0 g/L, KH\\u003csub\\u003e2\\u003c/sub\\u003ePO\\u003csub\\u003e4\\u003c/sub\\u003e 2.0 g/L, NH\\u003csub\\u003e4\\u003c/sub\\u003eNO\\u003csub\\u003e3\\u003c/sub\\u003e 1.0 g/L, MgSO\\u003csub\\u003e4\\u003c/sub\\u003e\\u0026middot;7H\\u003csub\\u003e2\\u003c/sub\\u003eO 0.1 g/L, FeSO\\u003csub\\u003e4\\u003c/sub\\u003e\\u0026middot;7H\\u003csub\\u003e2\\u003c/sub\\u003eO 0.01 g/L, ZnSO\\u003csub\\u003e4\\u003c/sub\\u003e\\u0026middot;7H\\u003csub\\u003e2\\u003c/sub\\u003eO 0.001 g/L, MnSO\\u003csub\\u003e4\\u003c/sub\\u003e\\u0026middot;6H\\u003csub\\u003e2\\u003c/sub\\u003eO 0.0002 g/L and CuSO\\u003csub\\u003e4\\u003c/sub\\u003e\\u0026middot;7H\\u003csub\\u003e2\\u003c/sub\\u003eO 0.0001 g/L (pH 7.5) (Oshiman et al. \\u003cspan citationid=\\\"CR38\\\" class=\\\"CitationRef\\\"\\u003e2007\\u003c/span\\u003e, Vargas-Su\\u0026aacute;rez et al. \\u003cspan citationid=\\\"CR46\\\" class=\\\"CitationRef\\\"\\u003e2019\\u003c/span\\u003e, Yang et al. \\u003cspan citationid=\\\"CR52\\\" class=\\\"CitationRef\\\"\\u003e2015\\u003c/span\\u003e). Additionally, 0.02 g of surface sterilized PLA film (\\u0026asymp;\\u0026thinsp;1 \\u0026times; 2 cm\\u003csup\\u003e2\\u003c/sup\\u003e) was added to the medium as the sole carbon source. The PLA films were sterilized by rinsing with 75% ethanol, washing with sterile distilled water, drying in a laminar airflow cabinet, and then exposing to UV irradiation for 10 minutes. The enrichment transfers were conducted by adding 2 mL of the pelagic microbes and PLA film from the previous culture to fresh 25 mL of MSM. This process was repeated every 7 days of incubation at 30\\u0026deg;C for a total of five consecutive transfers. After the fifth transfer, the bacteria were isolated and purified. The enrichment culture was spread using the standard dilution-plating technique on Nutrient Broth (NB) agar (Mistry et al. \\u003cspan citationid=\\\"CR34\\\" class=\\\"CitationRef\\\"\\u003e2022\\u003c/span\\u003e). The NB agar medium was prepared by dissolving 10.0 g of peptone, 3.0 g of beef powder, 5.0 g of sodium chloride, and 20 g of agar in one liter of distilled water (pH 7.2). After 72 h of incubation at 30 ℃, individual colonies were randomly selected and repeatedly streaked on the NB agar to obtain pure colonies. The isolate, designated as strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e, was cultured on NB medium for additional taxonomic experiments and preserved as both NB medium slants at 4 ℃ and suspensions with 20% (v/v) glycerol at -80 ℃.\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec4\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003ePhylogeny\\u003c/h2\\u003e \\u003cp\\u003eFor phylogenetic analysis of the 16S rRNA gene, PCR amplification and sequencing were carried out following a previously described method (Xia et al. \\u003cspan citationid=\\\"CR50\\\" class=\\\"CitationRef\\\"\\u003e2020\\u003c/span\\u003e). The obtained 16S rRNA sequence was aligned against the bacterial species recorded in the EzBioCloud database to determine the closest phylogenetic neighbors and their corresponding similarity (Yoon et al. \\u003cspan citationid=\\\"CR53\\\" class=\\\"CitationRef\\\"\\u003e2017a\\u003c/span\\u003e). Multiple sequence alignment of 16S rRNA gene sequence of the strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and its top 32 closest relatives with validly published names was performed by using CLUSTAL X 2.0 software (Larkin et al. \\u003cspan citationid=\\\"CR28\\\" class=\\\"CitationRef\\\"\\u003e2007\\u003c/span\\u003e). The aligned sequences were used to reconstruct the neighbour-joining (NJ) (Saitou and Nei \\u003cspan citationid=\\\"CR42\\\" class=\\\"CitationRef\\\"\\u003e1987\\u003c/span\\u003e) and maximum-likelihood (ML) (Felsenstein \\u003cspan citationid=\\\"CR19\\\" class=\\\"CitationRef\\\"\\u003e1981\\u003c/span\\u003e) phylogenetic trees using the program MEGA 11.0 (Tamura et al. \\u003cspan citationid=\\\"CR44\\\" class=\\\"CitationRef\\\"\\u003e2021\\u003c/span\\u003e), with bootstrap values based on 1000 replications. To further determine the classification position of CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e within \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e, comparative sequence analysis of the partial DNA gyrase subunit B (gyrB) gene and RNA polymerase β-subunit (rpoB) genes were performed. These genes are widely used as house-keeping genes for classification and phylogenetic markers of \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e (Bernard et al. \\u003cspan citationid=\\\"CR5\\\" class=\\\"CitationRef\\\"\\u003e2006\\u003c/span\\u003e, Bruno et al. \\u003cspan citationid=\\\"CR9\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e, Karah et al. \\u003cspan citationid=\\\"CR24\\\" class=\\\"CitationRef\\\"\\u003e2011\\u003c/span\\u003e, Nemec et al. \\u003cspan citationid=\\\"CR35\\\" class=\\\"CitationRef\\\"\\u003e2011\\u003c/span\\u003e, Nemec et al. \\u003cspan citationid=\\\"CR36\\\" class=\\\"CitationRef\\\"\\u003e2009\\u003c/span\\u003e). Multilocus sequence analysis (MLSA) of concatenated housekeeping gene sequences has proven to be a valuable method for classifying genera and species within the family \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e (Bruno et al. \\u003cspan citationid=\\\"CR9\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e, Karah et al. \\u003cspan citationid=\\\"CR24\\\" class=\\\"CitationRef\\\"\\u003e2011\\u003c/span\\u003e).\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec5\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003eGenome analysis\\u003c/h2\\u003e \\u003cp\\u003eThe draft genome of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was obtained using the Whole Genome Shotgun (WGS) strategy, which involved constructing libraries with different insertion fragments and performing paired-end (PE) sequencing on the Illumina NovaSeq platform using Next Generation Sequencing (NGS) technology. For high-throughput sequencing, a TruSeqTM DNA Sample Prep Kit was used to construct DNA sequencing libraries following the standard Illumina TruSeq Nano DNA LT library preparation process (Illumina TruSeq DNA Sample Preparation Guide). Subsequently, the paired-end sequencing libraries (2\\u0026times;150 bp) were sequenced using the Illumina NovaSeq platform. Reads were processed and assembled de novo using A5-MiSeq (Coil et al. \\u003cspan citationid=\\\"CR15\\\" class=\\\"CitationRef\\\"\\u003e2015\\u003c/span\\u003e) version 20160825 and SPAdes (Bankevich et al. \\u003cspan citationid=\\\"CR3\\\" class=\\\"CitationRef\\\"\\u003e2012\\u003c/span\\u003e) v3.12.0 (\\u003cspan class=\\\"ExternalRef\\\"\\u003e\\u003cspan class=\\\"RefSource\\\"\\u003ehttp://cab.spbu.ru/files/release3.12.0/manual.html\\u003c/span\\u003e\\u003cspan address=\\\"http://cab.spbu.ru/files/release3.12.0/manual.html\\\" targettype=\\\"URL\\\" class=\\\"RefTarget\\\"\\u003e\\u003c/span\\u003e\\u003c/span\\u003e). Based on the results of A5-MiSeq, base correction was performed using Pilon (Walker et al. \\u003cspan citationid=\\\"CR48\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e) software v1.18. Open reading frames, rRNA, tRNA and CRISPRs were predicted and annotated using the GeneMarkS v4.32, Barrnap v0.9, tRNAscan-SE v1.3.1 and CRISPRCasFinder v4.2.20 (Besemer et al. \\u003cspan citationid=\\\"CR6\\\" class=\\\"CitationRef\\\"\\u003e2001\\u003c/span\\u003e, Couvin et al. \\u003cspan citationid=\\\"CR16\\\" class=\\\"CitationRef\\\"\\u003e2018\\u003c/span\\u003e, Kalvari et al. \\u003cspan citationid=\\\"CR23\\\" class=\\\"CitationRef\\\"\\u003e2018\\u003c/span\\u003e, Lowe and Eddy \\u003cspan citationid=\\\"CR31\\\" class=\\\"CitationRef\\\"\\u003e1997\\u003c/span\\u003e). The protein sequences encoded by the gene were compared with the protein sequences in the NCBI NR, eggNOG (evolutionary genealogy of genes: Non-supervised Orthologous Groups), KEGG (Kyoto Encyclopedia of Genes and Genomes), Swiss-Prot and GO (Gene Ontology) databases using diamond blastp, and the cut-off value of sequence alignment was selected as 1e-6, and the best Hits were selected for function discrimination. To further confirm that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was a novel species of the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e, genome sequencing of the strain was performed, and in silico DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) tests were conducted between strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and the closest related type species. Silico ANI and DDH values were calculated using the EzBioCloud web server (\\u003cspan class=\\\"ExternalRef\\\"\\u003e\\u003cspan class=\\\"RefSource\\\"\\u003ehttps://www.ezbiocloud.net/tools/ani)(Yoon et al. 2017b)\\u003c/span\\u003e\\u003cspan address=\\\"https://www.ezbiocloud.net/tools/ani)(Yoon et al. 2017b)\\\" targettype=\\\"URL\\\" class=\\\"RefTarget\\\"\\u003e\\u003c/span\\u003e\\u003c/span\\u003e with the recommended algorithm, and the Genome-to-Genome Distance Calculator version 3.0 was used with the recommended Formula 2 (\\u003cspan class=\\\"ExternalRef\\\"\\u003e\\u003cspan class=\\\"RefSource\\\"\\u003ehttp://ggdc.dsmz.de\\u003c/span\\u003e\\u003cspan address=\\\"http://ggdc.dsmz.de\\\" targettype=\\\"URL\\\" class=\\\"RefTarget\\\"\\u003e\\u003c/span\\u003e\\u003c/span\\u003e) (Meier-Kolthoff et al. \\u003cspan citationid=\\\"CR33\\\" class=\\\"CitationRef\\\"\\u003e2013\\u003c/span\\u003e).\\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec6\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003ePhenotypic and chemotaxonomic characterization\\u003c/h2\\u003e \\u003cp\\u003eGrowth tests were performed at 30\\u0026deg;C for 7 days on various media, including Brain-Heart Infusion (BHI) agar, R2A agar, tryptic soy agar (TSA), Luria\\u0026ndash;Bertani (LB) agar and NB agar. Colony morphology was observed after 48 hours growth on NA plate at 37 ℃. Cell motility was assessed through motility assays in semi-solid medium(including 0.5% agar) and the hanging drop method. Gram staining was performed by using commercial kits as per according to the manufacturer\\u0026rsquo;s guidelines. Cell morphology were observed using scanning electron microscopy (SEM, SU8010, HITACH) after incubation in NB culture to mid-exponential phase at 30 ℃. Growth at different temperature (4, 8, 16, 20, 25, 30, 37, 40, 45, 50 and 60 ℃) in NB medium and at various pH values (pH 4.0\\u0026ndash;11.0, prepared with appropriate biological buffers at intervals of 0.5 unit) in NB medium at 30 ℃ were determined after 48 hours of incubation. Salt tolerance was determined after 48 h of incubation in NB medium supplemented with 0\\u0026ndash;15% (w/v) NaCl (at 1.5% intervals). All physiological tests were carried out in triplicates, and growth was measured turbidometrically (OD) at 600 nm using a spectrophotometer and cuvette of 1.0 cm path length. Catalase activity was determined by the observation of the bubble production in a 3% (v/v) hydrogen peroxide solution, while oxidase activity was examined using 1% (w/v) tetramethyl-ρ-phenylenediamine. Acid production from carbohydrates were examined with the API 50CH system (bioM\\u0026eacute;rieux), while other biochemical and enzymatic tests were performed with the API 20NE system (bioM\\u0026eacute;rieux) and API ZYM system (bioM\\u0026eacute;rieux) following the manufacturer\\u0026rsquo;s instructions. Anaerobic growth was assessed by incubating inoculated NB agar plates in Mitsubishi\\u0026trade; AnaeroPouch-Bag at 30℃ for 7 days. Duplicate tests for antibiotic susceptibility of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e were conducted using the disc diffusion method (Bauer et al. \\u003cspan citationid=\\\"CR4\\\" class=\\\"CitationRef\\\"\\u003e1966\\u003c/span\\u003e) on NB agar at 30℃ with filter paper discs containing various antibiotics and respective concentrations: carbenicillin (100 \\u0026micro;g), ampicillin (10 \\u0026micro;g), kanamycin (30 \\u0026micro;g), cefatriaxone (30 \\u0026micro;g), cefoperazone (75 \\u0026micro;g), ceftazidime (30 \\u0026micro;g), cefazolin (30 \\u0026micro;g), gentamicin (10 \\u0026micro;g), penicillin (10 U), cefuroxime (30 \\u0026micro;g), neomycin (30 \\u0026micro;g), cephalexin (30 \\u0026micro;g), doxycycline (30 \\u0026micro;g), tetracycline (30 \\u0026micro;g), piperacillin (100 \\u0026micro;g), topaminokana (30 \\u0026micro;g), erythromycin (15 \\u0026micro;g), cefradine (30 \\u0026micro;g), minocycline (30 \\u0026micro;g), oxacillin(1 \\u0026micro;g), chloramphenicol (20 \\u0026micro;g), spectacomycin (200 \\u0026micro;g), gentamicin (200 \\u0026micro;g).\\u003c/p\\u003e \\u003cp\\u003eFor the determination of the whole-cell fatty acid composition, strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was cultivated on NB agar at 30 ℃ for 48 hours. The fatty acids were then extracted and analyzed according to the recommendations of the Microbial Identification System (MIDI), a commercial identification system. The whole fatty acid composition was determined using an Agilent 6890N gas chromatograph. For the analysis of lipids, cells were cultured in NB culture until the mid-exponential phase on a rotary shaker at 30 ℃. Polar lipids were extracted from 200 mg of dry cells as described by Bligh and Dyer (BLIGH and DYER \\u003cspan citationid=\\\"CR7\\\" class=\\\"CitationRef\\\"\\u003e1959\\u003c/span\\u003e), followed by separation using two-dimensional thin-layer chromatography (TLC) on silica gel GF254 plates (100\\u0026times;100 mm; haiyangchem). The solvent system used for separation has been previously described (Tindall et al. \\u003cspan citationid=\\\"CR45\\\" class=\\\"CitationRef\\\"\\u003e2007\\u003c/span\\u003e).\\u003c/p\\u003e \\u003cp\\u003eFor the determination of the whole-cell fatty acid composition, strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was cultivated on NB agar at 30 ℃ for 48 hours. The fatty acids were then extracted and analyzed according to the recommendations of the Microbial Identification System (MIDI), a commercial identification system. The whole fatty acid composition was determined using an Agilent 6890N gas chromatograph. For the analysis of lipids, cells were cultured in NB culture until the mid-exponential phase on a rotary shaker at 30 ℃. Polar lipids were extracted from 200 mg of dry cells as described by Bligh and Dyer (BLIGH and DYER \\u003cspan citationid=\\\"CR7\\\" class=\\\"CitationRef\\\"\\u003e1959\\u003c/span\\u003e), followed by separation using two-dimensional thin-layer chromatography (TLC) on silica gel GF254 plates (100\\u0026times;100 mm; haiyangchem). The solvent system used for separation has been previously described (Tindall et al. \\u003cspan citationid=\\\"CR45\\\" class=\\\"CitationRef\\\"\\u003e2007\\u003c/span\\u003e).\\u003c/p\\u003e \\u003c/div\\u003e\"},{\"header\":\"Results and Discussion\",\"content\":\"\\u003cdiv id=\\\"Sec8\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003ePhylogeny analysis\\u003c/h2\\u003e \\u003cp\\u003eThe full-length sequence of 16S rRNA gene (1459 bp) extracted from the draft genome of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e showed the highest similarity with \\u003cem\\u003eA. gerneri\\u003c/em\\u003e DSM 14967\\u003csup\\u003eT\\u003c/sup\\u003e (97.74%), followed by \\u003cem\\u003eA. kanungonis\\u003c/em\\u003e PS-1\\u003csup\\u003eT\\u003c/sup\\u003e (97.60%) and \\u003cem\\u003eA. tandoii\\u003c/em\\u003e DSM 14970\\u003csup\\u003eT\\u003c/sup\\u003e (97.53%). The ML phylogenetic tree based on 16S rRNA gene sequences (Fig.\\u0026nbsp;\\u003cspan refid=\\\"Fig1\\\" class=\\\"InternalRef\\\"\\u003e1\\u003c/span\\u003e) showed that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e clustered with related species from the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e. The consistent clustering pattern could also be observed in the NJ phylogenetic tree (Fig. \\u003cspan refid=\\\"MOESM1\\\" class=\\\"InternalRef\\\"\\u003eS1\\u003c/span\\u003e). It was obvious that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e belongs to the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e. The MLSA-derived ML phylogenetic tree (Fig.\\u0026nbsp;\\u003cspan refid=\\\"Fig2\\\" class=\\\"InternalRef\\\"\\u003e2\\u003c/span\\u003e) demonstrates that strain CAAS 2-6T forms a separate subclade when compared to the 53 other strains of the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e. The similar clustering pattern is observed in the MLSA-derived NJ phylogenetic tree (Fig. \\u003cspan refid=\\\"MOESM2\\\" class=\\\"InternalRef\\\"\\u003eS2\\u003c/span\\u003e). Therefore, our MLSA results suggested that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e is a member of the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e close to \\u003cem\\u003eA. radioresistens\\u003c/em\\u003e, but representing a distinct species.\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec9\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003eGenome features\\u003c/h2\\u003e \\u003cp\\u003eA total of 1.235 Gb of raw DNA sequence was generated, which was assembled into 73 scaffolds and 469\\u0026times; coverage. The assembled draft genome of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was estimated to be at least 2,593,745 bp in size with a G\\u0026thinsp;+\\u0026thinsp;C content of 44.41%. In total, 2,344 genes, 59 tRNA, 3 rRNA and 0 CRISPRs were predicted in the assembled draft genome. NR annotations of protein-coding genes are shown in Table \\u003cspan refid=\\\"MOESM1\\\" class=\\\"InternalRef\\\"\\u003eS1\\u003c/span\\u003e. The four databases (NR, eggNOG, KEGG, and Swiss-Prot) annotated 2,344, 2,089, 1,783, and 1,803 gene functions, respectively, and these functional genes were classified and statistically displayed in Fig.\\u0026nbsp;\\u003cspan refid=\\\"Fig3\\\" class=\\\"InternalRef\\\"\\u003e3\\u003c/span\\u003e. The genome sequence of CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e has been deposited in the DDBJ/ ENA/GenBank under accession No. JAQIHG000000000. Genome sequences of closely related taxa were obtained from the NCBI GenBank database. In silico DDH tests revealed that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e had the highest genomes similarity of 22.7% with \\u003cem\\u003eA. indicus\\u003c/em\\u003e CIP 110367\\u003csup\\u003eT\\u003c/sup\\u003e. The DNA relatedness values of CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e with other \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e species ranged from 19.7 to 22.3% (Table \\u003cspan refid=\\\"MOESM2\\\" class=\\\"InternalRef\\\"\\u003eS2\\u003c/span\\u003e). Furthermore, strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e showed the highest genome similarity of 77.00% with \\u003cem\\u003eA. indicus\\u003c/em\\u003e CIP 110367\\u003csup\\u003eT\\u003c/sup\\u003e. and the ANI values between strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and other \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e species ranged from 74.49 to 76.59% (Table \\u003cspan refid=\\\"MOESM2\\\" class=\\\"InternalRef\\\"\\u003eS2\\u003c/span\\u003e). The DDH values and ANI values between strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and other type species in the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e were lower than the generally accepted species-level boundary of 70% and 95\\u0026thinsp;\\u0026minus;\\u0026thinsp;6%, respectively (Chun et al. \\u003cspan citationid=\\\"CR14\\\" class=\\\"CitationRef\\\"\\u003e2018\\u003c/span\\u003e). Based on these findings, it can be concluded that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e represents a previously unidentified species within the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e.\\u003c/p\\u003e \\u003cp\\u003e \\u003c/p\\u003e \\u003c/div\\u003e \\u003cdiv id=\\\"Sec10\\\" class=\\\"Section2\\\"\\u003e \\u003ch2\\u003ePhenotypic and Chemotaxonomic characterization\\u003c/h2\\u003e \\u003cp\\u003eColonies of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e on NB agar were circular, smooth and 0.8\\u0026ndash;1.2 mm in diameter. Strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was Gram-staining-negative, aerobic, and short rod shaped, with a size of approximately 0.7 \\u0026micro;m\\u0026times;0.9\\u0026ndash;1.6 \\u0026micro;m (Fig. S3). Strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was able to grow at 15\\u0026ndash;40 ℃ (optimally at 25\\u0026ndash;40 ℃), at pH 6.0\\u0026ndash;8.0 (optimally at pH 7.0\\u0026ndash;7.5) and in the presence of 0\\u0026ndash;6% (w/v) NaCl (optimally at 0\\u0026ndash;4%). Strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e exhibited resistance to Penicillin, Ceftazidime, and Oxacillin(=\\u0026thinsp;0 cm), showed weak resistance to Ampicillin, Cefazolin, Gentamicin, Neomycin, Cephalexin, and Cefradine(\\u0026le;\\u0026thinsp;1.5 cm), while being susceptible to the remaining 14 antibiotics. Differential phenotypic characteristics between strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and its closer affinity species of the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e were summarized in Table\\u0026nbsp;\\u003cspan refid=\\\"Tab1\\\" class=\\\"InternalRef\\\"\\u003e1\\u003c/span\\u003e. The API ZYM, API 20NE and API 50CH results confirmed that the classification of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e as a member of the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e. For example, all known species within the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e lack the ability to produce arginine hydrolase, chymotrypsin α-galactosidase, β-galactosidase, α-glucosidase, β-glucosidase, β-glucuronidase, N-acetyl-β-glucosaminase, α-mannosidase, and β-fucosidase. Conversely, they exhibit the ability to produce esterase and leucine-arylamidase, lipase, valine arylamidase and cystinol-arylamidase. However, strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e differed from all recognized species of genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e. It could be distinguishable from other species in genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e by the ability to ferment glucose, as well as the absence of acid phosphomonoesterase activity (Table S3).\\u003c/p\\u003e \\u003cp\\u003eThe whole-cell fatty acid profiles of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and other species in the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e are presented in Table\\u0026nbsp;2. Strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e exhibited the similarly predominant fatty acids, including C\\u003csub\\u003e12:0\\u003c/sub\\u003e(7.0%), C\\u003csub\\u003e16:0\\u003c/sub\\u003e(25.7%), C\\u003csub\\u003e16:0\\u003c/sub\\u003e N alcohol(6.5%), C\\u003csub\\u003e18:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e9\\u003cem\\u003ec\\u003c/em\\u003e(10.5%) and Summed Feature 3(25.8%). The detailed fatty acid profile of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e can be found in Table\\u0026nbsp;\\u003cspan refid=\\\"Tab1\\\" class=\\\"InternalRef\\\"\\u003e1\\u003c/span\\u003e. The polar lipid composition mainly consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and phosphatidylcholine(PC) (Fig. S4). These profiles were similar to those of recently described strains wihin the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e (Das et al. \\u003cspan citationid=\\\"CR18\\\" class=\\\"CitationRef\\\"\\u003e2021\\u003c/span\\u003e, Feng et al. \\u003cspan citationid=\\\"CR20\\\" class=\\\"CitationRef\\\"\\u003e2014\\u003c/span\\u003e, Malhotra et al. \\u003cspan citationid=\\\"CR32\\\" class=\\\"CitationRef\\\"\\u003e2012\\u003c/span\\u003e). The analysis of chemotaxonomic properties, including fatty acid profiles and polar lipid composition, confirmed the placement of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e within the genus Acinetobacter as a novel species.\\u003c/p\\u003e \\u003cp\\u003e \\u003cdiv class=\\\"gridtable\\\"\\u003e\\u003ctable float=\\\"Yes\\\" id=\\\"Tab1\\\" border=\\\"1\\\"\\u003e \\u003ccaption language=\\\"En\\\"\\u003e \\u003cdiv class=\\\"CaptionNumber\\\"\\u003eTable 1\\u003c/div\\u003e \\u003cdiv class=\\\"CaptionContent\\\"\\u003e \\u003cp\\u003eFatty acid composition of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e and its phylogenetically closest relatives Strains: 1, CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e; 2, \\u003cem\\u003eA. chinensis\\u003c/em\\u003e WCHAc010005\\u003csup\\u003eT\\u003c/sup\\u003e; 3, \\u003cem\\u003eA. sichuanensis\\u003c/em\\u003e WCHAc060041\\u003csup\\u003eT\\u003c/sup\\u003e; 4, \\u003cem\\u003eA. chengduensis\\u003c/em\\u003e WCHAc060005\\u003csup\\u003eT\\u003c/sup\\u003e; 5, \\u003cem\\u003eA. wuhouensis\\u003c/em\\u003e WCHA60\\u003csup\\u003eT\\u003c/sup\\u003e. All dates from this study. \\u0026ndash;, Not detected. Fatty acids present at \\u0026gt;\\u0026thinsp;5 % are highlighted in bold. Summed Feature 2 comprised C\\u003csub\\u003e12:0\\u003c/sub\\u003e aldehyde ?, Summed Feature 3 comprised C\\u003csub\\u003e16:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e7\\u003cem\\u003ec\\u003c/em\\u003e and/or C\\u003csub\\u003e16:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e6\\u003cem\\u003ec\\u003c/em\\u003e, Summed Feature 5 comprised C\\u003csub\\u003e18:2\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e6, 9\\u003cem\\u003ec\\u003c/em\\u003e and/or Ante-C\\u003csub\\u003e18:0\\u003c/sub\\u003e, Summed Feature 8 comprised C\\u003csub\\u003e18:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e7\\u003cem\\u003ec\\u003c/em\\u003e.\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/caption\\u003e \\u003ccolgroup cols=\\\"6\\\"\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c1\\\" colnum=\\\"1\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c2\\\" colnum=\\\"2\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c3\\\" colnum=\\\"3\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c4\\\" colnum=\\\"4\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c5\\\" colnum=\\\"5\\\"\\u003e\\u003c/div\\u003e \\u003cdiv align=\\\"left\\\" class=\\\"colspec\\\" colname=\\\"c6\\\" colnum=\\\"6\\\"\\u003e\\u003c/div\\u003e \\u003cthead\\u003e \\u003ctr\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eFatty acid\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e2\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e3\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e4\\u003c/p\\u003e \\u003c/th\\u003e \\u003cth align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e5\\u003c/p\\u003e \\u003c/th\\u003e \\u003c/tr\\u003e \\u003c/thead\\u003e \\u003ctbody\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e10:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e0.22\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e0.55\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e0.19\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e1.20\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e0.34\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e12:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e6.96\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e12.06\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e5.61\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e13.60\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e13.68\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eIso-C\\u003csub\\u003e13:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.82\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e3.17\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e12:0\\u003c/sub\\u003e 2-OH\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e0.51\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e1.75\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e2.05\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e12:0\\u003c/sub\\u003e 3-OH\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e3.37\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e7.94\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e7.04\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e5.87\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eIso-C\\u003csub\\u003e14:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.29\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e1.90\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e0.72\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e14:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.20\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e0.50\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e9.78\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e1.72\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e0.96\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eIso-C\\u003csub\\u003e15:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e3.84\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e1.98\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e0.65\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eAnteiso-C\\u003csub\\u003e15:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.32\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e1.45\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e6.33\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e16:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e7\\u003cem\\u003ec\\u003c/em\\u003e alcohol\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.77\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e16:0\\u003c/sub\\u003e N alcohol\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e6.45\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eIso-C\\u003csub\\u003e16:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.55\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e0.44\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e4.68\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e16:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e25.74\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e17.90\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e25.60\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e19.17\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e14.46\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eIso-C\\u003csub\\u003e17:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e0.10\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e1.48\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e0.29\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e0.83\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e0.82\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eIso-C\\u003csub\\u003e17:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e10\\u003cem\\u003ec\\u003c/em\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.06\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e9.13\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e17:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e0.19\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e0.83\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e0.94\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e1.18\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e18:3\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e6\\u003cem\\u003ec\\u003c/em\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e2.08\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e18:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e9\\u003cem\\u003ec\\u003c/em\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e10.46\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e24.31\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e19.84\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e18.18\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e18:0\\u003c/sub\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e1.06\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e0.90\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e1.53\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e2.44\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e2.86\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eC\\u003csub\\u003e19:0\\u003c/sub\\u003e cyclo \\u003cem\\u003eω\\u003c/em\\u003e8\\u003cem\\u003ec\\u003c/em\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e1.36\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eSummed Feature 2\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e0.54\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e12.79\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e0.54\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eSummed Feature 3\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e25.78\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e27.47\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e10.76\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e20.40\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e25.82\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eSummed Feature 5\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e0.22\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003ctr\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c1\\\"\\u003e \\u003cp\\u003eSummed Feature 8\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c2\\\"\\u003e \\u003cp\\u003e0.60\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c3\\\"\\u003e \\u003cp\\u003e4.50\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c4\\\"\\u003e \\u003cp\\u003e\\u003cb\\u003e21.78\\u003c/b\\u003e\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c5\\\"\\u003e \\u003cp\\u003e\\u0026ndash;\\u003c/p\\u003e \\u003c/td\\u003e \\u003ctd align=\\\"left\\\" colname=\\\"c6\\\"\\u003e \\u003cp\\u003e2.50\\u003c/p\\u003e \\u003c/td\\u003e \\u003c/tr\\u003e \\u003c/tbody\\u003e \\u003c/colgroup\\u003e \\u003c/table\\u003e\\u003c/div\\u003e \\u003c/p\\u003e \\u003cp\\u003e \\u003cb\\u003eDescription of A\\u003c/b\\u003e \\u003cb\\u003ecinetobacter lentus\\u003c/b\\u003e \\u003cb\\u003esp. nov.\\u003c/b\\u003e\\u003c/p\\u003e \\u003cp\\u003e \\u003cem\\u003eAcinetobacter lentus\\u003c/em\\u003e (len\\u0026rsquo;tus. L. masc. adj. lentus, slow, delayed, referring to slow growth).\\u003c/p\\u003e \\u003cp\\u003eCells are Gram-staining-negative, aerobic, non-motile, short rods (approx. 0.7 \\u0026micro;m\\u0026times;0.9\\u0026ndash;1.6 \\u0026micro;m). Growth occurs on BHI agar, R2A agar, TSA, NA and LB agar. Colonies grown on NB agar medium are circular, smooth, and 0.8\\u0026ndash;1.2 mm in diameter within 24 hours at 37 ℃. Growth occurs at 15\\u0026ndash;40 ℃ (optimally at 25\\u0026ndash;40 ℃), at pH 6.0\\u0026ndash;8.0 (optimally at pH 7.0\\u0026ndash;7.5) and in the presence of 0\\u0026ndash;6% (w/v) NaCl (optimally at 0\\u0026ndash;4%). Positive results were obtained for catalase, alkaline phosphomonoesterase, esterase(C4 and C8), leucine-arylamidase, valine arylamidase and Naphthol-AS-BI-phosphoric acid, negative results were observed for oxidase, arginine dihydrolase, urease, gelatinase, lipase(C14), cystinol- arylamidase, trypsin, chymotrypsin, acid phosphomonoesterase, α-galactosidase, β-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, N-acetyl-β-glucosaminase, α-mannosidase and β-fucosidase. In the API 20NE system, assimilation occurred on L-arabinose, D-mannose, N-acetyl glucosamine, D-maltose, gluconate, decanoic acid, malic acid, citric acid and phenylacetic acid. In the API 50CH system, acids are produced from glucose, furctoseribose, N-acetylglucosamine, esculin, maltose, starch; are weakly produced from arbutin; and are not produced from glycerinum, erythrose, D-arabinose, L-arabinose, ribose, D-xylose, L-xylose, adonitol, β-methyl-D-xyloside, galactose, mannose, sorbose, rhamnose, dulcitol, inositol, mannitol, sorbitol, α-metyl-D-mannosidase, α-metyl-D-glucoside, amygdalin, salicoside, cellobiose, lactose, melibiose, sucrose, trehalose, inulin, melezitose, raffinose, glycogen, xylitol, geranyl, D-turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, gluconat, 2-keto-gluconate, 5-keto-gluconate. The predominant fatty acids were identified as C\\u003csub\\u003e12:0\\u003c/sub\\u003e, C\\u003csub\\u003e16:0\\u003c/sub\\u003e N alcohol, C\\u003csub\\u003e16:0\\u003c/sub\\u003e, C\\u003csub\\u003e18:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e6\\u003cem\\u003ec\\u003c/em\\u003e, and Summed Feature 3. Polar lipids include DPG, PG, and PC.\\u003c/p\\u003e \\u003cp\\u003eThe type strain, CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e (=\\u0026thinsp;GDMCC 1.3951\\u003csup\\u003eT\\u003c/sup\\u003e\\u0026thinsp;=\\u0026thinsp;KCTC 8156\\u003csup\\u003eT\\u003c/sup\\u003e\\u0026thinsp;=\\u0026thinsp;JCM 36321\\u003csup\\u003eT\\u003c/sup\\u003e), was isolated from Chang'an Landfill in the Longquan Mountains, China. The DNA G\\u0026thinsp;+\\u0026thinsp;C content of the type strain is 44.41 mol%. The GenBank accession numbers for the draft genome assembly and 16S rRNA gene sequences of strain \\u003cem\\u003eAcinetobacter lentus\\u003c/em\\u003e CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e are JAQIHG000000000 and OQ110573 respectively.\\u003c/p\\u003e \\u003c/div\\u003e \"},{\"header\":\"Abbreviations\",\"content\":\"\\u003cdiv class=\\\"DefinitionList\\\"\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eANI\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eaverage nucleotide identity\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eBHI\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eBrain-Heart Infusion\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eCRISPRs\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eClustered Regularly Interspaced Short Palindromic Repeats\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003edDDH\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003edigital DNA\\u0026ndash;DNA hybridization\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eMSM\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003emineral salt medium\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eGO\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eGene Ontology\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eGDMCC\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eGuangdong Microbial Culture Collection Center\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eJCM\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eJapan Collection of Microorganisms\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eKCTC\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eKorean Collection for Type Cultures\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eKEGG\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eKyoto Encyclopedia of Genes and Genomes\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eMEGA\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eMolecular Evolutionary Genetics Analysis\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eLB\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eLuria-Bertani\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eNB\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eNutrient Broth\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eML\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003emaximum-likelihood\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eNJ\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eneighbor-joining\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eMIDI\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eMicrobial Identification System\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eDPG\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003ediphosphatidylglycerol\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003ePG\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003ephosphatidylglycerol\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003ePC\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003ephosphatidylcholine\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eTLC\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003eThin layer chromatography\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003cdiv class=\\\"DefinitionListEntry\\\"\\u003e \\u003cdiv class=\\\"Term\\\"\\u003eTSA\\u003c/div\\u003e \\u003cdiv class=\\\"Description\\\"\\u003e \\u003cp\\u003etryptic soy agar.\\u003c/p\\u003e \\u003c/div\\u003e \\u003c/div\\u003e \\u003c/div\\u003e\"},{\"header\":\"Declarations\",\"content\":\"\\u003cp\\u003eWe sincerely appreciate the help of Associate Research Shichun Ma\\u0026apos;s team from the Biogas Science Research Institute of the Chinese Academy of Agricultural Sciences in using the MIDI system for fatty acids composition determination and polar lipids identification.\\u003c/p\\u003e\\n\\u003cp\\u003e \\u003ch2\\u003eConflicts of interest\\u003c/h2\\u003e \\u003cp\\u003eThe authors have no relevant financial or non-financial interests to disclose.\\u003c/p\\u003e \\u003c/p\\u003e\\u003ch2\\u003eFunding information\\u003c/h2\\u003e \\u003cp\\u003eThis work was financially supported by National Natural Science Foundation of China (No. 31600012), Sichuan science and technology program (2018JY0216, 2022YFS0508), Qinghai Science and Technology program (2022-NK-124) and chengdu science and technology program (2022-YF05-00753).\\u003c/p\\u003e\\u003ch2\\u003eAuthor Contribution\\u003c/h2\\u003e\\u003cp\\u003eAll authors contributed to the study conception and design. Mengli Xia performed the experiment and writing - original draft, Yuandong Zhao performed the experiment, Bo Wu contributed significantly to analysis and manuscript preparation, Guoquan Hu performed the data analyses, Mingxiong He conceptualization and supervision, Yanwei Wang conceptualization and supervision. All authors read and approved the final manuscript.\\u003c/p\\u003e\\u003ch2\\u003eData Availability\\u003c/h2\\u003e \\u003cp\\u003eThe GenBank/EMBL/DDBJ accession number for the genome and 16S rRNA gene sequences of strain \\u003cem\\u003eAcinetobacter lentus\\u003c/em\\u003e CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e are JAQIHG000000000 and OQ110573 respectively.\\u003c/p\\u003e\"},{\"header\":\"References\",\"content\":\"\\u003col\\u003e\\u003cli\\u003e\\u003cspan\\u003eAlvarez-Perez, S., Baker, L.J., Morris, M.M., Tsuji, K., Sanchez, V.A., Fukami, T., Vannette, R.L., Lievens, B. and Hendry, T.A. (2021) Acinetobacter pollinis sp. nov., Acinetobacter baretiae sp. nov. and Acinetobacter rathckeae sp. nov., isolated from floral nectar and honey bees. 71(5).\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003e\\u0026Aacute;lvarez-P\\u0026eacute;rez, S., Lievens, B., Jacquemyn, H. and Herrera, C.M. (2013) Acinetobacter nectaris sp. nov. and Acinetobacter boissieri sp. nov., isolated from floral nectar of wild Mediterranean insect-pollinated plants. 63(Pt_4), 1532\\u0026ndash;1539.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eBankevich, A., Nurk, S., Antipov, D., Gurevich, A.A., Dvorkin, M., Kulikov, A.S., Lesin, V.M., Nikolenko, S.I., Pham, S., Prjibelski, A.D., Pyshkin, A.V., Sirotkin, A.V., Vyahhi, N., Tesler, G., Alekseyev, M.A. and Pevzner, P.A. 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Implications for finding sequence motifs in regulatory regions. 29(12), 2607\\u0026ndash;2618.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eBLIGH, E.G. and DYER, W.J. (1959) A rapid method of total lipid extraction and purification. 37(8), 911\\u0026ndash;917.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eBRISOU, J. and PREVOT, A.R. (1954) Studies on bacterial taxonomy. X. The revision of species under Acromobacter group. 86(6), 722\\u0026ndash;728.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eBruno, P., Sylvie, G., Violaine, W., Lenka, K., Gustavo, C., Cheryl, M., Michael, F., Jennifer, W., Dominique, C., Alexandr, N. and Patrice, C. 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(2020) Acinetobacter portensis sp. nov. and Acinetobacter guerrae sp. nov., isolated from raw meat. 70(8), 4544\\u0026ndash;4554.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eChen, X.-M., An, D.-F., He, S.-R., Yang, S.-J., Yang, Z.-Z., Xiong, L.-S., Li, G.-D., Jiang, M.-G., Jiang, C.-L. and Jiang, Y. (2022) Acinetobacter faecalis Sp. Nov., Isolated from Elephant Faeces. 80(1), 21.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eChoi, J.Y., Ko, G., Jheong, W., Huys, G., Seifert, H., Dijkshoorn, L. and Ko, K.S. (2013) Acinetobacter kookii sp. nov., isolated from soil. 63(Pt_12), 4402\\u0026ndash;4406.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eChun, J., Oren, A., Ventosa, A., Christensen, H., Arahal, D.R., da Costa, M.S., Rooney, A.P., Yi, H., Xu, X.-W., De Meyer, S. and Trujillo, M.E. (2018) Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. 68(1), 461\\u0026ndash;466.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eCoil, D., Jospin, G. and Darling, A.E. (2015) A5-miseq: an updated pipeline to assemble microbial genomes from Illumina MiSeq data. 31(4), 587\\u0026ndash;589.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eCouvin, D., Bernheim, A., Toffano-Nioche, C., Touchon, M., Michalik, J., N\\u0026eacute;ron, B., Rocha, E.P.C., Vergnaud, G., Gautheret, D. and Pourcel, C. (2018) CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins. Nucleic Acids Research 46(W1), W246-W251.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eDahal, R.H., Chaudhary, D.K. and Kim, J. (2017) Acinetobacter halotolerans sp. nov., a novel halotolerant, alkalitolerant, and hydrocarbon degrading bacterium, isolated from soil. 199(5), 701\\u0026ndash;710.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eDas, L., Deb, S. and Das, S.K. (2021) Description of Acinetobacter kanungonis sp. nov., based on phylogenomic analysis. 71(6).\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eFelsenstein, J. (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. 17(6), 368\\u0026ndash;376.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eFeng, G.-D., Yang, S.-Z., Wang, Y.-H., Deng, M.-R. and Zhu, H.-H. 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(2014) Draft Genome Sequence of the Mercury-Resistant Bacterium Acinetobacter idrijaensis Strain MII, Isolated from a Mine-Impacted Area, Idrija, Slovenia. 2(6), \\u003cspan class=\\\"ExternalRef\\\"\\u003e\\u003cspan class=\\\"RefSource\\\"\\u003e10.1128/genomea.01177\\u0026thinsp;\\u0026ndash;\\u0026thinsp;01114\\u003c/span\\u003e\\u003cspan address=\\\"10.1128/genomea.01177\\u0026thinsp;\\u0026ndash;\\u0026thinsp;01114\\\" targettype=\\\"DOI\\\" class=\\\"RefTarget\\\"\\u003e\\u003c/span\\u003e\\u003c/span\\u003e.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eKalvari, I., Argasinska, J., Quinones-Olvera, N., Nawrocki, E.P., Rivas, E., Eddy, S.R., Bateman, A., Finn, R.D. and Petrov, A.I. 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(2017a) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. 67(5), 1613\\u0026ndash;1617.\\u003c/span\\u003e\\u003c/li\\u003e \\u003cli\\u003e\\u003cspan\\u003eYoon, S.-H., Ha, S.-m., Lim, J., Kwon, S. and Chun, J. (2017b) A large-scale evaluation of algorithms to calculate average nucleotide identity. 110(10), 1281\\u0026ndash;1286.\\u003c/span\\u003e\\u003c/li\\u003e\\u003c/ol\\u003e\"}],\"fulltextSource\":\"\",\"fullText\":\"\",\"funders\":[],\"hasAdminPriorityOnWorkflow\":false,\"hasManuscriptDocX\":true,\"hasOptedInToPreprint\":true,\"hasPassedJournalQc\":\"\",\"hasAnyPriority\":false,\"hideJournal\":true,\"highlight\":\"\",\"institution\":\"\",\"isAcceptedByJournal\":false,\"isAuthorSuppliedPdf\":false,\"isDeskRejected\":\"\",\"isHiddenFromSearch\":false,\"isInQc\":false,\"isInWorkflow\":false,\"isPdf\":false,\"isPdfUpToDate\":true,\"isWithdrawnOrRetracted\":false,\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"researchsquare\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":true,\"externalIdentity\":\"\",\"sideBox\":\"\",\"snPcode\":\"\",\"submissionUrl\":\"/submission\",\"title\":\"Research Square\",\"twitterHandle\":\"researchsquare\",\"acdcEnabled\":true,\"dfaEnabled\":false,\"editorialSystem\":\"\",\"reportingPortfolio\":\"\",\"inReviewEnabled\":false,\"inReviewRevisionsEnabled\":true},\"keywords\":\"\",\"lastPublishedDoi\":\"10.21203/rs.3.rs-3910326/v1\",\"lastPublishedDoiUrl\":\"https://doi.org/10.21203/rs.3.rs-3910326/v1\",\"license\":{\"name\":\"CC BY 4.0\",\"url\":\"https://creativecommons.org/licenses/by/4.0/\"},\"manuscriptAbstract\":\"\\u003cp\\u003eThe taxonomic position of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e, a Gram-negative, aerobic, non-motile, mesophilic bacterium with positive catalase and negative oxidase activity, isolated from Chang'an Landfill in the Longquan Mountains, China. The 16S rRNA gene sequence (1459 bp) of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e showed high sequence similarity to \\u003cem\\u003eA. gerneri\\u003c/em\\u003e DSM 14967\\u003csup\\u003eT\\u003c/sup\\u003e (97.74%), followed by \\u003cem\\u003eA. kanungonis\\u003c/em\\u003e PS-1\\u003csup\\u003eT\\u003c/sup\\u003e (97.60%) and \\u003cem\\u003eA. tandoii\\u003c/em\\u003e DSM 14970\\u003csup\\u003eT\\u003c/sup\\u003e (97.53%). Phylogenetic analyses based on 16S rRNA, rpoB and gyrB gene sequences, together with DNA\\u0026ndash;DNA hybridization values less than 70 %, indicated that strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e belongs to the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e and may represent a novel species. Genome sequencing revealed a genome size of 4.829 Mbp and a G\\u0026thinsp;+\\u0026thinsp;C content of 57.13 mol%. Major fatty acids identified were Summed Feature 3 (25.78%), C\\u003csub\\u003e16:0\\u003c/sub\\u003e (25.74%), C\\u003csub\\u003e18:1\\u003c/sub\\u003e \\u003cem\\u003eω\\u003c/em\\u003e9\\u003cem\\u003ec\\u003c/em\\u003e (10.46%), C\\u003csub\\u003e12:0\\u003c/sub\\u003e (6.96%) and C\\u003csub\\u003e16:0\\u003c/sub\\u003e N alcohol (6.45%). The polar lipid profile of strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e revealed the presence of phosphatidylglycerol, phosphatidylcholine, and diphosphatidylglycerol. Based on phenotypic, phenotypic and biochemical characteristics, strain CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e was clearly distinct from the type strains of other recognized species in the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e, suggesting that it represents a novel species of the genus \\u003cem\\u003eAcinetobacter\\u003c/em\\u003e. Therefore, the name \\u003cem\\u003eAcinetobacter lentus\\u003c/em\\u003e sp. nov. is proposed. The type strain is CAAS 2-6\\u003csup\\u003eT\\u003c/sup\\u003e (=\\u0026thinsp;GDMCC 1.3951\\u003csup\\u003eT\\u003c/sup\\u003e\\u0026thinsp;=\\u0026thinsp;KCTC 8156\\u003csup\\u003eT\\u003c/sup\\u003e\\u0026thinsp;=\\u0026thinsp;JCM 36321\\u003csup\\u003eT\\u003c/sup\\u003e).\\u003c/p\\u003e\",\"manuscriptTitle\":\"Acinetobacter lentus sp. nov. isolated from Landfill\",\"msid\":\"\",\"msnumber\":\"\",\"nonDraftVersions\":[{\"code\":1,\"date\":\"2024-02-01 09:00:50\",\"doi\":\"10.21203/rs.3.rs-3910326/v1\",\"editorialEvents\":[{\"type\":\"communityComments\",\"content\":0}],\"status\":\"published\",\"journal\":{\"display\":true,\"email\":\"info@researchsquare.com\",\"identity\":\"researchsquare\",\"isNatureJournal\":false,\"hasQc\":true,\"allowDirectSubmit\":true,\"externalIdentity\":\"\",\"sideBox\":\"\",\"snPcode\":\"\",\"submissionUrl\":\"/submission\",\"title\":\"Research Square\",\"twitterHandle\":\"researchsquare\",\"acdcEnabled\":true,\"dfaEnabled\":false,\"editorialSystem\":\"\",\"reportingPortfolio\":\"\",\"inReviewEnabled\":false,\"inReviewRevisionsEnabled\":true}}],\"origin\":\"\",\"ownerIdentity\":\"4909b5f4-7d84-424f-88df-0b278cb55290\",\"owner\":[],\"postedDate\":\"February 1st, 2024\",\"published\":true,\"recentEditorialEvents\":[],\"rejectedJournal\":[],\"revision\":\"\",\"amendment\":\"\",\"status\":\"posted\",\"subjectAreas\":[],\"tags\":[],\"updatedAt\":\"2024-07-23T13:38:06+00:00\",\"versionOfRecord\":[],\"versionCreatedAt\":\"2024-02-01 09:00:50\",\"video\":\"\",\"vorDoi\":\"\",\"vorDoiUrl\":\"\",\"workflowStages\":[]},\"version\":\"v1\",\"identity\":\"rs-3910326\",\"journalConfig\":\"researchsquare\"},\"__N_SSP\":true},\"page\":\"/article/[identity]/[[...version]]\",\"query\":{\"redirect\":\"/article/rs-3910326\",\"identity\":\"rs-3910326\",\"version\":[\"v1\"]},\"buildId\":\"qtupq5eGEP_6zYnWcrvyt\",\"isFallback\":false,\"isExperimentalCompile\":false,\"dynamicIds\":[84888],\"gssp\":true,\"scriptLoader\":[]}","source_license":"CC-BY-4.0","license_restricted":false}