{"paper_id":"fb165f82-fb77-4691-9f5f-0621d8ff5391","body_text":"Summary\nIn order to investigate the mechanism of elevated vascular endothelial growth factor (VEGF) in peritoneal fluids from patients with endometriosis, macrophages were recovered from peritoneal fluids obtained at the time of diagnostic laparoscopy from infertile women with endometriosis (EMT group,n=20) and without endometriosis (control group,n=20). Macrophages were culturedin vitro. The VEGF levels of peritoneal fluid and the supernatant of macrophages culture were determined by enzyme linked immunoassay (ELISA). Meanwhile, the eutopic (n=20) and ectopic endometrium (n=20) from endometriosis patients, and normal edometrium (n=20) from non-endometriosis patients were obtained for the analysis of VEGF expression by labeled Streptavidin Biotin (LSAB). It was found that VEGF levels in peritoneal fluid and macrophages culture supernatant were significantly higher in EMT group than in control group (P<0.01). In normal endometrium, VEGF showed a cyclic changes and similar in eutopic and ectopic endometrium from patients with endometriosis. There was no difference in the intensity of VEGF in endometrium between two groups within each menstrual phase. It is suggested that altered VEGF production by peritoneal macrophages and ectopic endometrium secretion may contribute to the elevated VEGF levels in the peritoneal fluid of patients with endometriosis.\nSimilar content being viewed by others\nReferences\nMclaren J, Prentice A, Channock-jones D Set al. Vascular endothelial growth factor concentrations are elevated in peritoneal fluid of women with endometriosis. Hum Reprod, 1996,11:220\nAmerican Fertility Society. Revised American fertility classification of endometriosis 1985. Fertil Steril, 1985, 43:351\nThomas E J, Prentice A. The aetiology and pathogenesis of endometriosis. Reprod Med Rev, 1992,1:21\nNisolle M, Casans-Roux F, Anaf Vet al. Morphometric study of the stromal vascularization in peritoneal endometriossis. Fertil Steril, 1993,59:681\nOosterlynck D, Menleman C, Sobis Het al. Angiogenic activity of peritoneal fluid from women with endometriosis. Fertil Steril, 1993,59:778.\nRyan I, Tseng J, Schriock Eet al. Interleukin-8 concentrations are elevated in peritoneal fluid of women with endometriosis. Fertil Steril, 1995,61:929\nSmith S K. Angiogenesis. Semin, Reprod Endocrinol, 1998,15:221\nEschen A, Duclos B, Schmitt-Goguel Net al. Human resident peritoneal macrophages: phneotype and biology. Br J Haematol, 1994,88:712\nAuthor information\nAuthors and Affiliations\nAdditional information\nLIU Yi, male, born in 1968. Associate Professor\nRights and permissions\nAbout this article\nCite this article\nYi, L., Liqun, L.V. Mechanism of elevated vascular endothelial growth factor levels in peritoneal fluids from patients with endometriosis. Current Medical Science 24, 470–472 (2004). https://doi.org/10.1007/BF02831111\nReceived:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/BF02831111","source_license":"CC0","license_restricted":false}