{"paper_id":"f021f8c7-44d9-4d17-aec1-983854d0fe04","body_text":"Abstract\nPurpose\nEndometriosis (EMT) is a chronic benign disease with high prevalence. This study investigated the diagnostic value of serum miR-17-5p, miR-424-5p, and their combined expressions for EMT.\nMethods\nTotal 80 EMT patients of reproductive age who underwent laparoscopy or laparotomy and were confirmed by pathological examination were included as the study subjects, and another 80 healthy women of reproductive age receiving gynecological examination and ultrasonography with no pelvic abnormalities were selected as the control group. The whole blood samples of enrolled subjects were collected and clinical characteristics were recorded. The miR-17-5p, miR-424-5p, VEGFA, IL-4, and IL-6 levels in the serum were measured. ROC curve was used to evaluate the diagnostic efficacy of miR-17-5p and miR-424-5p expressions for EMT. Pearson correlation was performed to analyze the correlation of miR-17-5p and miR-424-5p with clinical indexes in EMT patients.\nResults\nmiR-17-5p and miR-424-5p were downregulated in EMT patients. For diagnosing EMT, the AUC of miR-17-5p was 0.865 and cutoff value was 0.890 (91.3% sensitivity and 85% specificity), the AUC of miR-424-5p was 0.737, and cutoff value was 0.915 (98.8% sensitivity and 61.2% specificity), and the AUC of miR-424-5p combined with miR-17-5p was 0.938 and cutoff value was 2.205 (93.8% sensitivity and 88.7% specificity), with the diagnostic efficacy higher than miR-424-5p or miR-17-5p alone. miR-17-5p and miR-424-5p expressions were negatively correlated with dysmenorrhea, infertility, pelvic pain, and rASRM stage, but not with age, BMI, menstrual disorder, and nulliparity. VEGFA, IL-4, IL-6, and CA-125 were increased in EMT patients and were inversely associated with miR-17-5p and miR-424-5p.\nConclusion\nmiR-424-5p combined with miR-17-5p has high diagnostic efficacy for EMT.\nSimilar content being viewed by others\nAvailability of data and materials\nAll the data generated or analyzed during this study are included in this published article.\nReferences\nGarcia-Ibanez P, Yepes-Molina L, Ruiz-Alcaraz AJ, Martinez-Esparza M, Moreno DA, Carvajal M, Garcia-Penarrubia P (2020) Brassica bioactives could ameliorate the chronic inflammatory condition of endometriosis. Int J Mol Sci. https://doi.org/10.3390/ijms21249397\nGolabek A, Kowalska K, Olejnik A (2021) Polyphenols as a diet therapy concept for endometriosis-current opinion and future perspectives. 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Hum Reprod 30:2292–2302. https://doi.org/10.1093/humrep/dev204\nAcknowledgements\nNot applicable.\nFunding\nNot applicable.\nAuthor information\nAuthors and Affiliations\nContributions\nCLL is the guarantor of integrity of the entire study; CLL contributed to the study concepts, study design, definition of intellectual content, literature research, clinical studies, experimental studies, data acquisition, data analysis, statistical analysis, manuscript preparation, manuscript editing and manuscript review; SLZ contributed to the study concepts, study design, definition of intellectual content, literature research, clinical studies, data acquisition, data analysis, manuscript preparation, manuscript editing and manuscript review; MJL contributed to the study design, clinical studies, experimental studies, data acquisition, data analysis, statistical analysis, manuscript preparation, manuscript editing and manuscript review; all authors read and approved the final manuscript.\nCorresponding authors\nEthics declarations\nCompeting interest\nThe authors declare that they have no conflicts of interest.\nEthics statement\nThis study was approved by the academic ethics committee of Hunan Province Maternal and Child Health care Hospital and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. All participants were fully informed and voluntarily signed the informed consent before sampling.\nInformed consent\nInformed consent was obtained from all individual participants included in the study.\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nAbout this article\nCite this article\nLin, C., Zeng, S. & Li, M. miR-424-5p combined with miR-17-5p has high diagnostic efficacy for endometriosis. Arch Gynecol Obstet 307, 169–177 (2023). https://doi.org/10.1007/s00404-022-06492-6\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-022-06492-6","source_license":"public-domain-us","license_restricted":false}