{"paper_id":"effe9bdc-adab-40a0-ac7b-204b529ebc2b","body_text":"Summary\nTo study the changes of cytokines in peritoneal fluids of patients with endometriosis and their effects on reproductive activity, levels of tumor necrosis factor (TNF) and interleukin 6 (IL-6) in peritoneal fluids and peritoneal macrophages’ culture supernatant were studied by using enzyme linked immunoassay (ELISA) in 14 infertile patients with endometriosis (EMT group) and 11 infertile women with normal pelvis (control group). The effects of peritoneal fluids in patients with endometriosisin vitro on sperm motility and development of 2-cell mouse embryos were also studied. The results showed that the levels of TNF and IL-6 in peritoneal fluids and peritoneal macrophages’ supernatant in EMT group were elevated significantly as compared with those in the control group (P <0. 01). The percent of sperm straight line movement and total sperm motility were decreased significantly in EMT group (P<0.01). The percent of 2-cell mouse embryos developing to 16-cells was 32.5% in EMT group, while 47.6% in control group after 48 h co-culture with peritoneal fluid (P <0.01). It is likely that the elevation of peritoneal fluid cytokines in patients with endometriosis may play a role in infertility associated with endometriosis.\nSimilar content being viewed by others\nReferences\nAmerican Fertility Society. Revised American Fertility classification of endometriosis 1985. Fertil Steril, 1985, 43:351\nKoama N, Matsura K, Okamura H. Cytokines in the peritoneal fluid of patients with endometriosis. Int J Gynecol Obstet, 1993, 43:45\nBoutten A, Dehoux F, Proserpio Pet al. IL-6 and acute phase protein in the peritoneal fluid of women with endometriosis/ Clin Chem Acta, 1992, 40:187\nVercellini P, De-Baenedetti F, Rossi E. Tumor necrosis factor in plasma and peritoneal fluid of women with or without endometriosis. Gynecol Obste Invest, 1993, 36: 39\nBuyalos R P, Funari V A, Azziz Ret al. Elevated interleukin 6 levels in peritoneal fluid of patients with pelvi pathology. Fertil Steril, 1992, 58:302\nCurtis P, Lindsay A E, Jackson Fet al. Adverse effects on sperm movement characteristic in women with minimal and mild endometriosis. Br J Obstet Gynecol, 1993, 100:165\nLeach R E, Ameson B W, David Ball Get al. Absence of antisperm antibodies and factors influencing sperm motility in the cul-de-sac fluid of women with endometriosis. Fertil Steril, 1990, 53:351\nTaketani Y, Kuo T M, Miauno M. Comparison of cytokine levels and embryo toxicity in peritoneal fluid in infertile women with untreated or treated endometriosis. Am J Obstet Gynecol, 1992, 167:265\nEisermann J, Register K B, Strickler R Cet al. The effect of tumor necrosis factor on human sperm motility in vitro. J Androl, 1989, 10:270\nTaketani Y, Kuo T M, Mizuno M. Tumor necrosis factor inhibits the development of mouse embryos cocultured with oviducts: possible relevance to infertility with endometriosis. J Mamm OV Res, 1991, 8:175\nAuthor information\nAuthors and Affiliations\nRights and permissions\nAbout this article\nCite this article\nYi, L., Lilan, L. & Haibo, Z. Changes of cytokines levels in peritoneal fluids of patients with endometriosis and its effect on reproductive activity. Current Medical Science 20, 163–165 (2000). https://doi.org/10.1007/BF02887062\nReceived:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/BF02887062","source_license":"CC0","license_restricted":false}