{"paper_id":"ef510515-f71a-4bd4-803e-39bc2db52c2d","body_text":"Adenomyosis was first identified at the end of the 19th century in uteri examined post-hysterectomy ( Bird  et al. , 1972 ). More recently, advances in medical imaging have enabled non-invasive diagnosis, underscoring that adenomyosis also affects younger, reproductive-age women ( Pinzauti  et al. , 2015 ;  Chapron  et al. , 2017 ;  García-Solares  et al. , 2018 ). Clinically, the condition is often associated with pelvic pain, abnormal uterine bleeding, and multiparity ( Vannuccini and Petraglia, 2019 ;  Vercellini  et al. , 2025 ). Since the initial theory positing that endometrial tissue penetrates the myometrium through a compromised junctional zone ( Bergeron  et al. , 2006 ;  Stratopoulou  et al. , 2021 ), numerous molecular pathways have been explored to elucidate pathogenesis.\nOver the past decade, murine models, particularly tamoxifen-induced models, have emerged as key research platforms for dissecting adenomyosis mechanisms ( Parrott  et al. , 2001 ;  Green  et al. , 2005 ). Although multiple mechanisms contribute to lesion progression, immune dysregulation has emerged as a critical driver ( Li  et al. , 2024 ). Bourdon  et al.  highlighted immunological disturbances and aberrant Notch1 signaling near or during the implantation window ( Bourdon  et al. , 2021 ,  2024 ). However, our group was the first to demonstrate a direct fertility impairment in tamoxifen-induced adenomyosis mice, documenting disrupted estrous cyclicity, prolonged estrus, impaired folliculogenesis, and notably smaller litter sizes and numbers ( Squatrito  et al. , 2024 ). We further linked these reproductive defects to progesterone resistance and reduced endometrial receptivity, as evidenced by downregulation of progesterone receptor expression, HoxA10, and integrin β3.\nDespite these advances, the precise pathways by which adenomyosis impairs fertility remain incompletely defined. Several studies have implicated eutopic-endometrial immune dysregulation as a key contributor ( Barrier  et al. , 2004 ;  Campo  et al. , 2012 ;  Bourdon  et al. , 2021 ;  Stratopoulou  et al. , 2023 ), and mounting evidence indicates that inflammation disrupts processes crucial for successful implantation and pregnancy maintenance ( Guo  et al. , 2018 ). However, most work has focused on immune changes at or after conception, leaving a gap in our understanding of pre-conception immune alterations.\nAddressing this gap is crucial for identifying early pathogenic events and for pinpointing the most effective strategies to safeguard fertility. Therefore, the aim of the present study was to investigate the uterine immune environment prior to pregnancy during the development of the pathology in tamoxifen-induced adenomyosis mice. By defining the timing and nature of immune dysregulation in adenomyosis, we aim to refine our understanding of its pathogenesis and highlight potential avenues for intervention. Ultimately, pinpointing the onset of these immune disturbances may pave the way for preventive measures to counteract the reduced fertility often observed in adenomyosis.\n\nAnimal experiments in this study were approved by the Animal Ethics Committee of the University of Liege (approval number # 2387) and conducted following ARRIVE guidelines. In total, 70 neonatal female CD1 mice were included in the study and divided into two groups. The first group received an oral administration of vehicle from postnatal day (PND) 1–5, while the second group received an oral administration of tamoxifen (2.7 µmol/kg = 1 mg/kg) (Sigma, T5648, St Louis, MO, USA) suspended in a peanut oil/lecithin/condensed milk mixture (2:0.2:3, v/v) at a dose volume of 5 µl/g body weight. This tamoxifen treatment was used to induce adenomyosis as previously described ( Green  et al. , 2005 ) ( Fig. 1 ).\nExperimental design.  Schematic overview of the experimental design and analysis workflow in control (CTL) and adenomyosis (ADM) mice at 1- and 3-month post-induction. Created in BioRender. Munaut, C. (2025)  https://BioRender.com/3l3vb9j .\nAll mice were housed under controlled conditions, with a temperature maintained at approximately 21°C and a 12-h light/dark cycle, with ad libitum access to food and water. Mice were sacrificed at two different time points: an early stage, 4 weeks after treatment initiation, where 16 mice per group were euthanized, and an advanced stage, 12 weeks after treatment initiation, where 20 mice from the vehicle group and 18 mice from the tamoxifen group were sacrificed ( Fig. 1 ). These time points were selected based on our previous characterization of this model: at 1-month post-induction, mice consistently display early disruption of the myometrium without invasive adenomyotic lesions (pre-lesional stage), whereas by 3-month post-induction, adenomyotic lesions are confirmed in nearly all animals, with the majority reaching grade 3 severity ( Squatrito  et al. , 2024 ). These two windows thus allow capturing both the onset of uterine alterations and the established disease stage, providing a relevant framework to study temporal immune remodeling.\nTo minimize variability related to the estrous cycle, mice were synchronized by subcutaneous injection of 17β-estradiol (100 ng/100 ml) for three consecutive days before sacrifice, as previously described ( De Clercq  et al. , 2017 ).\nMice were first anesthetized with isoflurane inhalation (5% in oxygen) until loss of pedal reflex, and subsequently euthanized by cervical dislocation, in accordance with the AVMA Guidelines for the Euthanasia of Animals ( Leary  et al ., 2020 ) and the approved institutional protocol.\nFollowing euthanasia, uterine tissues were collected for further analysis. To ensure enough immune cells for flow cytometry analysis, one-half uterine horn from two mice were pooled. The remaining uterine horn from each mouse was divided into two (1-month-old mice) or three (3-month-old mice) parts: one portion was snap-frozen in liquid nitrogen and then stored at −80°C for RNA extraction and quantitative real-time PCR (RT-qPCR) analyses, while the other was fixed in 4% formalin and embedded in paraffin for immunohistochemistry (IHC) analyses.\nTo confirm the presence or absence of adenomyotic lesions, double immunofluorescence staining was performed on uterine sections using αSMA-FITC (Sigma, F3777, 1/400, St Louis, MO, USA) and EpCAM (Cell Signaling, 93790, 1/400, Danvers, MA, USA) antibodies. αSMA antibody was directly conjugated to FITC, allowing visualization of the myometrium in green, while EpCAM was detected using a tyramide amplification system conjugated to Cy3, marking the epithelial glands in red.\nBriefly, paraffin-embedded uterine sections were first deparaffinized and rehydrated, followed by antigen retrieval using an autoclave (11 min, 126°C, 1.3 bar). After cooling for 20 min at room temperature (RT), endogenous peroxidase activity was blocked by incubating the sections in 3% hydrogen peroxide for 20 min at RT. To prevent non-specific binding, sections were incubated for 20 min at RT in the ‘Animal-Free Blocking Solution’ (Cell Signaling).\nPrimary antibodies were diluted in REAL antibody diluent (Dako, Santa Clara, CA, USA) and incubated for 1 h at RT. After primary antibody incubation, sections were incubated with a secondary antibody conjugated to horseradish peroxidase (EnVision/HRP, Dako) for 30 min at RT. The revelation of EpCAM was performed using a tyramide amplification system conjugated to Cy3 (PerkinElmer, Shelton, CT, USA), allowing visualization of the epithelial glands in red. Sections were then mounted using DAPI Fluoromount-G mounting medium (SouthernBiotech, Birmingham, AL, USA).\nFluorescently labeled sections were scanned using the SLIDEVIEW VS200 research slide scanner (Olympus, Antwerp, Belgium) equipped with a Uplan-XApo 20× 0.8 NA objective (Olympus).\nUterine tissue fragments from CD1 mice were placed in a digestion solution composed of Hank’s balanced salt solution (HBSS) and collagenase type Ia (2.5 mg/ml) and incubated at 37°C with agitation for 1 h. To facilitate tissue dissociation, pipetting (40 up-and-down motions with a P1000) was performed every 15 min. Following digestion, EDTA (1:100) was added, and additional pipetting was performed for 2 min to ensure complete tissue dissociation. The resulting cell suspension was filtered through a 70 µm mesh and centrifuged at 310 g  for 10 min at 4°C. The supernatant was discarded, and the pellet was resuspended in FACS buffer (HBSS supplemented with 2% FBS and 1% penicillin/streptomycin). To prevent non-specific binding, cells were incubated for 20 min at 4°C with a blocking solution (Fc block; 1:500 dilution in HBSS) prior to antibody staining. A mixture of primary antibodies, targeting various cell populations ( Table 1 ), was prepared in FACS buffer. Each sample was incubated with appropriate dilution of antibody mix in the dark, on ice, for 30 min, with gentle vortexing every 10 min. After incubation, cells were washed with FACS buffer and centrifuged at 250 g  for 5 min at 4°C. To exclude dead cells, a viability dye (Zombie Aqua) was used according to the manufacturer’s instructions (Biolegend, San Diego, CA, USA). To correct for spectral overlap between fluorophores, a compensation matrix was generated using single-stained beads labeled with individual fluorochromes. Fluorescence minus one (FMO) controls were systematically included for key markers to define gating thresholds. A uniform gating strategy was applied consistently across all samples within each experimental group, ensuring reliable discrimination of positive and negative subsets for each immune population. After staining, samples were filtered through 40 µm filters into fresh FACS tubes prior to analysis.\nAntibodies and viability dye used for flow cytometric analysis of mouse uterine immune cells.\nCell sorting was performed using an Aria III flow cytometer (BD Biosciences, San Jose, CA, USA), with all available cells from each sample being analyzed and sorted to maximize recovery of the targeted immune cell populations. Specifically, M1- and M2-like macrophage subsets were sorted with the initial aim of performing downstream RT-qPCR analyses to further characterize their polarization profiles. However, due to the low number of recovered cells in several samples, these downstream assays could not be reliably performed, and the present work therefore focuses on the flow cytometry-based characterization of the uterine immune microenvironment. One pooled sample (two uteri) was excluded from the analysis because the total number of recovered cells was abnormally low (<30 000 cells), whereas all other samples yielded a minimum of 600 000 cells analyzed. Flow cytometry data were analyzed using FlowJo software (version 10, Ashland, OR, USA). The gating strategies used for the flow cytometry analysis are shown in  Supplementary Fig. S1 .\nGene expression was assessed by RT-qPCR. Total RNA was extracted from individual samples using the RNeasy Mini kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.\nFor each sample, 1 µg of total RNA was reverse transcribed into complementary DNA (cDNA) using the FastGene Scriptase II Ready Mix (Nippon Genetics, Düren, Germany). RT-qPCR was performed using SYBR Green PCR Master Mix and specific primers ( Table 2 ) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA).\nPrimers for RT-qPCR.\nGene expression levels were analyzed using the 2 −ΔΔCt  method, with all samples measured in duplicate. To minimize variability related to the estrous cycle, only mice in estrus were selected for RT-qPCR analyses, since the expression of several genes, including inflammation-related genes, may fluctuate depending on cycle stage. To normalize gene expression,  Rplp0  and  Gapdh  were used as housekeeping genes, as recommended by Lin  et al.  for gene expression studies in the mouse uterus ( Lin  et al. , 2013 ).\nAll statistical analyses were performed using GraphPad Prism (version 9.0, San Diego, CA, USA).\nFor mouse and uterine weights, data were normally distributed, as assessed by the Shapiro–Wilk test and visual inspection of QQ plots. Therefore, results are presented as mean ± SD, and parametric unpaired two-tailed  t -tests were used to compare groups.\nFor flow cytometry (FACS) and RT-qPCR analyses, the non-parametric Mann–Whitney  U  test (two-tailed) was applied. In these cases, results are presented as medians (interquartile ranges).\nExact  P -values are reported in the text. A  P -value was considered statistically significant when  P  < 0.05. The number of replicates (biological or technical) is specified in each figure legend.\n\nTo assess adenomyosis progression, we analyzed uterine morphology in control (CTL) and tamoxifen-treated (ADM) mice at 1- and 3-month post-treatment.\nAt both time points, none of the control mice exhibited signs of adenomyosis, confirming that the condition does not spontaneously develop in ‘young’ untreated animals. In contrast, uteri from ADM mice sacrificed 30-day post-treatment displayed early signs of myometrial remodeling, characterized by tissue disorganization and structural alterations. However, in most cases, these modifications had not yet progressed into fully developed adenomyotic lesions, suggesting an initial phase of disease establishment without substantial glandular invasion.\nBy 90-day post-treatment, ADM mice exhibited prominent adenomyosis lesions, with the majority classified as grade 3, consistent with previously published data ( Squatrito  et al. , 2024 ). This progressive histopathological transformation supports the hypothesis that neonatal tamoxifen exposure induces sustained uterine alterations that gradually lead to advanced adenomyosis ( Fig. 2 ).\nUterine morphology in control (CTL) and adenomyosis (ADM) mice.  Representative immunofluorescence staining of transverse uterine sections at 1 and 3 months. aSMA (green) marks the myometrium, EpCAM (red) labels epithelial glands, and DAPI (blue) stains cell nuclei. At 1 month, CTL uteri display normal morphology ( A ), whereas ADM uteri show disrupted myometrium ( B , higher magnification). At 3 months, CTL uteri remain normal ( C ), while ADM uteri present adenomyotic lesions ( D , higher magnification). In (D), the myometrium–endometrium boundary is delineated (white circle). Scale bar, 500 µm.\nIn addition to morphological changes, physiological parameters were also affected. At 1-month post-treatment, body weight was significantly lower in ADM mice (22.86 ± 2.34 g) compared to controls (24.71 ± 1.55 g;  P  = 0.0133). However, this difference was no longer observed at 3 months (ADM: 34.98 ± 5.00 g vs CTL: 33.52 ± 3.45 g; n.s.), suggesting a transient growth delay. In contrast, uterine weight remained significantly reduced in ADM mice at both time points. At 1 month, ADM mice exhibited a dramatic reduction (0.036 ± 0.013 g) compared to controls (0.096 ± 0.010 g;  P  < 0.0001). A significant difference persisted at 3 months (ADM: 0.093 ± 0.036 g vs CTL: 0.145 ± 0.044 g;  P  = 0.0004), indicating sustained disruption of uterine homeostasis following neonatal tamoxifen exposure. Consistently, the uterine weight-to-body weight ratio was also significantly lower in ADM compared to CTL mice at both 1 month (ADM: 0.0016 ± 0.0006 vs CTL: 0.004 ± 0.0004;  P  < 0.0001) and 3 months (ADM: 0.0028 ± 0.001 vs CTL: 0.0044 ± 0.001;  P  = 0.0002), further confirming impaired uterine development ( Table 3 ).\nBody and uterine weights in control (CTL) and tamoxifen-treated (ADM) mice at 1- and 3-month post-treatment.\nData are expressed as mean ± standard deviation (SD). Statistical significance was assessed using unpaired two-tailed  t -tests.\n*  P  < 0.05, ***  P  < 0.001, ****  P  < 0.0001; n.s. = not significant.\nTogether, these findings provide insight into the temporal dynamic of adenomyosis development following neonatal tamoxifen exposure and highlight key histopathological and physiological alterations that precede the establishment of severe disease.\nAdenomyosis induces profound modulation of the uterine immune microenvironment, a phenomenon suspected to impair fertility. To characterize these immunological alterations, we profiled innate and adaptive immune cell subsets in uteri from CTL and ADM mice at 1 month (early stage) and 3 months (advanced stage) post-induction. Multiparametric flow cytometry enabled precise quantification of each subset and revealed time-dependent alterations in both innate and adaptive immune compartments, as detailed below.\nAt both early (1 month) and advanced (3 months) stages, the proportion of uterine NK cells (CD3 −  NKp46 + ) remained comparable between ADM and CTL mice ( Fig. 3A–C, M–O ), with no statistically significant differences ( P  = 0.2440 and  P  = 0.237, respectively). These findings suggest that adenomyosis does not substantially impact NK-cell-mediated immune surveillance at either time point.\nInnate immune cells in control (CTL) and adenomyosis (ADM) uteri.  Representative flow cytometry dot plots and violin plots showing the percentage of natural killer (NK) cells (CD3 −  NKp46 + ) ( A–C ,  M–O ), total macrophages (CD11b +  F4/80 + ) ( D–F ,  P–R ), M1-like macrophages (CD11b +  F4/80 +  CD86 + ) ( G–I ,  S–U ), and M2-like macrophages (CD11b +  F4/80 +  CD163 + ) ( J–L ,  V–X ) at 1 and 3 months. Adenomyosis induced an early reduction of total macrophages with an increase in the M2-like subset, followed by a decrease in M1-like macrophages 3-month post-tamoxifen treatment; NK-cell frequencies remained unchanged across time points. Sample sizes: 1 month (CTL 16 mice pooled (n = 8); ADM 16 mice pooled with one exclusion (n = 7)); 3 months (CTL 20 mice pooled (n = 10); ADM 18 mice pooled (n = 9)). Statistical significance was determined using the Mann–Whitney  U  test: * P  < 0.05, ** P  < 0.01; ns, not significant.\nAt the early stage, the proportion of total macrophages (CD11b +  F4/80 + ) was significantly reduced in ADM mice compared to CTL (median 9.27% vs 23.35%,  P  = 0.0093;  Fig. 3D–F ). This reduction was accompanied by a marked shift toward an increased frequency of M2-like macrophages (CD11b +  F4/80 +  CD163 + ) in ADM mice (median 20.9% vs 5.18%,  P  = 0.0012,  Fig. 2G–L ), while M1-like macrophages (CD11b +  F4/80 +  CD86 + ) remained comparable between groups.\nAt the advanced stage, the total macrophage population was no longer significantly different between ADM and CTL mice (median 15.70% vs 20.05%,  P  = 0.189,  Fig. 3P–R ). However, a significant decrease in M1-like macrophages was observed in the uteri of ADM mice compared to CTL mice (median 20.40% vs 31.85%,  P  = 0.035,  Fig. 3S–U ), whereas M2-like macrophages remained similar between groups ( Fig. 3V–X ).\nTogether, these results demonstrate a time-dependent modulation of macrophage polarization in ADM mice, with a significant reduction of M1-like macrophages and relatively stable levels of M2-like cells. This evolving macrophage profile is consistent with a progressive shift toward an anti-inflammatory immune environment in the adenomyotic uterus.\nAt the early stage (1 month), the proportion of uterine B cells (CD3 − CD19 + ) was significantly increased in ADM mice compared to CTL (median 0.56% vs 0.30%,  P  = 0.029). This elevation persisted at the advanced stage (3 months), with B-cell frequencies remaining significantly higher in ADM mice (median 1.36% vs 0.68%,  P  = 0.026,  Fig. 4A–C, J–L ). These findings indicate a sustained enrichment of B cells in the adenomyotic uterus, suggesting a long-lasting modulation of the humoral immune response in this pathological context.\nAdaptive immune cells in control (CTL) and adenomyosis (ADM) uteri.  Representative flow cytometry dot plots and violin plots showing the percentage of B cells (CD19 + ) and total T cells (CD3 + ) ( A–C ,  J–L ), helper T cells (CD4 + ) and cytotoxic T cells (CD8 + ) ( D–F ,  M–O ), and NKT cells (NKp46 +  CD3 + ) ( G–I ,  P–R ) at 1 and 3 months. ADM uteri showed increased B-cell percentage and higher CD4 +  T-cell levels at both time points, while CD8 +  T cells increased only 3-month post-tamoxifen treatment; total T and NKT cells remained unchanged. Sample sizes: 1 month (CTL 16 mice pooled (n = 8); ADM 16 mice pooled with one exclusion (n = 7)); 3 months (CTL 20 mice pooled (n = 10); ADM 18 mice pooled (n = 9)). Statistical significance was determined using the Mann–Whitney  U  test: * P  < 0.05, *** P  < 0.001; ns, not significant.\nWe first quantified total T lymphocytes (CD3 + CD19 − ) in uteri from CTL and ADM mice at 1 month (early stage) and 3 months (advanced stage) post-induction. The overall proportion of CD3 +  T cells did not differ significantly between ADM and CTL groups at either time point, indicating that adenomyosis does not affect total T-cell infiltration ( Fig. 4A–C, J–L ).\nWe then analyzed the distribution of four CD3 +  T-cell subsets based on CD4 and CD8 expression: double-negative (DN, CD4 − CD8 − ), CD4 single-positive (CD4 + CD8 − ), CD8 single-positive (CD4 − CD8 + ), and double-positive (CD4 + CD8 + ) cells ( Figs 4D–F, M–O  and  5 ).\nDistribution of uterine CD3 +  T-cell subsets.  Relative distribution of CD4 − CD8 −  (double-negative, DN), CD4 + CD8 − , CD4 − CD8 + , and CD4 + CD8 +  (double-positive, DP) T-cell subsets within the CD3 +  compartment at 1 and 3 months. ADM uteri showed a reduction of DN T cells and increased CD4 +  single-positive cells at both time points, while CD8 +  single-positive and DP T cells increased only 3-month post-tamoxifen treatment. Each bar represents the mean of individual percentages. Sample sizes: 1 month, CTL (16 pooled, n = 8), ADM (16 pooled, one exclusion, n = 7); 3 months, CTL (20 pooled, n = 10), ADM (18 pooled, n = 9). ADM, adenomyosis; CTL, control.\nAcross all groups, DN T cells represented the predominant subset, as shown by the 100% stacked bar graph ( Fig. 5 ). However, this population was significantly reduced in ADM mice compared to CTL both at 1 month (median 51.4% vs 68.85%;  P  = 0.0084) and at 3 months (median 47.3% vs 80.6%;  P  < 0.0001,  Table 4 ). Conversely, the frequency of CD4 + CD8 −  T cells was significantly increased in ADM mice at both time points (1 month:  P  = 0.0093; 3 months:  P  = 0.0002), as was the proportion of CD4 + CD8 +  double-positive cells (1 month:  P  = 0.5335; 3 months:  P  = 0.0128,  Table 4 ). No significant difference was observed in the CD8 + CD4 −  subset at 1 month ( P  = 0.5358), although a moderate increase was detected at 3 months in ADM mice ( P  = 0.0006).\nQuantitative comparison of CD3 +  T-cell subsets in the murine uterus at 1- and 3-month post-induction.\n68.85\n(66.55–70.50)\n25.15\n(21.63–28.55)\n4.80\n(3.78–5.61)\n0.48\n(0.41–1.48)\n51.40 **\n(38.8–8.30)\n44.70 **\n(37.60–53.50\n3.54\n(3.04–5.70)\n0.56\n(0.47–1.35)\n80.60\n(74.08–82.53)\n14.6\n(11.83–18.68)\n5.28\n(3.25–5.95)\n0.53\n(0.25–1.00)\n47.30 ****\n(38.40–60.95)\n41.00 ***\n(23.55–50.45)\n9.70 ***\n(8.58–11.60)\n1.96 *\n(1.12–4.25)\nMedian values (% of CD3 + ) with interquartile ranges (IQR) are presented. Statistical comparisons were performed using the Mann–Whitney  U  test between control (CTL) and adenomyosis (ADM) groups at each timepoint.\nP  < 0.05,\nP  < 0.01,\nP  < 0.001,\nP  < 0.0001.\nIn addition, natural killer T cells (NKT, CD3 +  NKp46 + ), a unique population bridging innate and adaptive immunity through rapid cytokine secretion, were also assessed. Their frequency remained low and did not differ significantly between ADM and CTL groups at either time point, suggesting that NKT cell involvement in the uterine immune response to adenomyosis is limited under these conditions.\nTo assess the cytokine and signaling profile associated with the altered immune landscape in adenomyotic uteri, we measured  Il6 ,  Il6R ,  Il10 ,  Stat3 , and  Ptgs2  mRNA levels by RT-qPCR in CTL and ADM mice at 1- and 3-month post-induction ( Fig. 6 ).\nExpression levels of inflammatory and immunoregulatory transcripts.  Relative mRNA expression of  Il6 ,  Il6r ,  Il10 ,  Stat3 , and  Ptgs2  in uterine tissues of control (CTL) and adenomyosis (ADM) mice at 1 and 3 months (RT-qPCR). At 1 month, ADM uteri showed increased expression of  Il6  and  Il10  with decreased expression of  Il6r  and  Stat3 , while  Ptgs2  expression remained unchanged; at 3 months, only  Il6  expression remained elevated. Bars represent median with interquartile range. Sample sizes: 1 month, CTL and ADM n = 12; 3 months, CTL and ADM n = 10.  P -values were calculated using the Mann–Whitney  U  test: * P  < 0.05, ** P  < 0.01, *** P  < 0.001.\nAt 1 month,  Il6  and  Il10  transcripts were significantly upregulated in ADM uteri compared to CTL ( Il6: P  = 0.0014;  Il10: P  = 0.0003), whereas  Il6R  and  Stat3  expression were significantly downregulated ( IL6R: P  = 0.0132;  Stat3: P  = 0.0205). In contrast,  Ptgs2  levels remained unchanged ( P  > 0.9999). At 3 months,  Il6  remained elevated in ADM mice ( Il6: P  = 0.003), but  Il6R ,  Stat3  and  Il10  expression were no longer different from CTL ( P  = 0.7759;  P  = 0.1431;  P  = 0.1564), and  Ptgs2  still showed no significant change ( P  = 0.7959).\nTogether, these data reveal a dysregulated cytokine signaling in adenomyotic uteri, marked by sustained  Il6  and  Il10  induction despite transient  Stat3  repression and no alteration of  Ptgs2  expression.\n\nIn this study, we investigated the temporal remodeling of the uterine immune microenvironment in a murine model of adenomyosis, integrating cellular and molecular analyses. Although flow cytometry was performed prior to gene expression analysis, the following overview synthesizes both datasets to provide a comprehensive picture of immune dynamics.\nOur data revealed coordinated alterations in both innate and adaptive compartments. Early adenomyosis was characterized by reduced macrophage numbers with a skewing toward M2-like polarization, while later stages showed selective depletion of M1-like macrophages. In parallel, B cells were consistently elevated, and CD3 +  T cells, although stable in total numbers, underwent a marked redistribution across subsets. At the molecular level,  Il6  mRNA was persistently upregulated, while  Il10  showed only transient elevation, and  Ptgs2  remained unchanged. Collectively, these findings suggest that adenomyosis provoked a progressive disruption of the innate–adaptive balance, shifting from an early compensatory anti-inflammatory response to a state of unresolved inflammation that may impair uterine homeostasis.\nFlow cytometry revealed a pronounced reshaping of CD3 +  T-cell subsets in the ADM uterus. The proportion of double-negative (CD4 − CD8 − ) cells significantly declined at both 1 and 3 months, consistent with a progressive depletion of this subset. In parallel, CD4 +  T cells increased significantly at both timepoints, suggesting early activation of helper T-cell responses. CD8 +  T cells showed a delayed but robust expansion at 3 months, indicative of cytotoxic effector engagement.\nNotably, CD4 + CD8 +  double-positive T cells emerged significantly at 3 months. These cells, typically rare in peripheral tissues, may represent transitional or activated phenotypes associated with chronic immune stimulation ( Parel and Chizzolini, 2004 ;  Hagen  et al. , 2023 ). This time-dependent redistribution of T-cell subsets coincides with transient  Stat3  downregulation, suggesting that dampened STAT3 signaling may influence lineage commitment toward CD4 +  and CD8 +  effector functions. Similar alterations affecting CD8 +  T cells have been reported in the endometrium of patients with adenomyosis and/or endometriosis, highlighting changes in cytotoxic T-cell populations associated with disease severity ( Kisovar  et al. , 2023 ;  Liu  et al. , 2023 ). Together, these data support the hypothesis of an evolving adaptive immune response in adenomyosis, potentially contributing to lesion persistence and fertility impairment.\nIn addition to immune remodeling, we also observed significant physiological alterations. ADM mice exhibited a transient reduction in body weight at 1-month post-induction, which normalized by 3 months. This early growth delay may reflect systemic effects of neonatal exposure or early inflammatory stress. More strikingly, uterine weight remained significantly reduced in ADM mice at both 1 and 3 months, suggesting a sustained disruption of uterine growth and tissue homeostasis. These changes parallel our molecular findings and support the idea that chronic immune imbalance may impair uterine remodeling, even beyond the acute lesion phase. These physiological observations are consistent with previous studies using neonatal tamoxifen to induce adenomyosis. Several reports have shown that uterine weight is significantly reduced in tamoxifen-treated mice at both early and late timepoints, even when body weight is unaffected ( Parrott  et al. , 2001 ;  Green  et al. , 2005 ). Our observation suggests that neonatal tamoxifen exposure disrupts uterine growth more specifically than systemic development.\nTogether, these immunological and physiological alterations underscore a coordinated disruption of uterine homeostasis in adenomyosis, prompting us to consider the underlying mechanisms by which IL6–driven inflammation and STAT3 modulation impair tissue growth and remodeling.\nOur data suggest that early innate changes establish the inflammatory context for adaptive responses. The reduction in macrophage numbers paired with M2 polarization may hinder clearance of ectopic endometrial cells while promoting tissue remodeling and fibrosis ( Gordon, 2003 ). Loss of M1‐like macrophages 3 months after adenomyosis induction may further diminish classical inflammatory functions necessary for lesion resolution ( Ota  et al. , 1998 ;  Riccio  et al. , 2018 ).\nParalleling endometriosis literature—where M2‐polarized macrophages accumulate in lesions and promote tissue invasion ( Bacci  et al. , 2009 ;  Nie  et al. , 2018 ), while studies on M1/M2 prevalence yield mixed findings ( Itoh  et al. , 2013 ;  Takebayashi  et al. , 2015 )—our adenomyosis model similarly exhibits an early M2 dominance followed by M1 loss. This dualistic concept has recently been challenged: macrophages can undergo ‘trained immunity’ and display hybrid M1/M2 signatures that defy a simple polarization framework ( Martinez and Gordon, 2014 ). These observations are consistent with human studies showing accumulation of M2 macrophages in adenomyotic lesions, where they contribute to tissue remodeling and lesion invasiveness ( Stratopoulou  et al. , 2023 ).\nThese macrophage dynamics appear to be closely tied to evolving cytokine environment. M1-like macrophages are known sources of pro-inflammatory cytokines such as IL-6, whereas M2-like macrophages secrete anti-inflammatory mediators like IL-10 ( Martinez and Gordon, 2014 ). At 1 month, we observed increased M2-like macrophages and elevated  Il10  mRNA, suggesting an early anti-inflammatory response. However,  Il6  was already significantly upregulated despite unchanged M1-like macrophage numbers, implying non-immune sources such as stromal or epithelial cells may contribute. By 3 months, M1-like macrophages were reduced and  Il10  levels normalized, whereas  Il6  remained elevated, pointing to a shift toward chronic, unresolved inflammation in the ADM uterus. Elevated  IL6  and altered  IL10  dynamics have also been reported in human endometrial tissues from patients with adenomyosis, supporting the translational relevance of these cytokine patterns, which are known to influence endometrial receptivity and implantation success ( Zhihong  et al. , 2016 ;  Wang  et al. , 2018 ).\nThese local and systemic immune changes have been associated with impaired fertility in a murine adenomyosis model, coinciding with alterations in local and systemic immune cells observed during the implantation window ( Bourdon  et al. , 2024 ).\nOur observation of a sustained increase in  Il6  mRNA in ADM uteri at both 1- and 3-month post-adenomyosis induction aligns with clinical reports of elevated IL-6 in the peritoneal fluid and lesional endometrium of patients with adenomyosis and endometriosis ( Incognito  et al. , 2023 ;  Cao  et al. , 2025 ). IL-6 is a pleiotropic cytokine that drives chronic inflammation by promoting STAT3 phosphorylation, leukocyte recruitment, angiogenesis, and stromal cell proliferation ( Tanaka  et al. , 2014 ;  West, 2019 ).\nAs an activator of the JAK–STAT signaling pathway, IL-6 can act in both a pro-inflammatory and anti-inflammatory manner. Deregulation of IL-6 production can cause severe disease, such as rheumatoid arthritis, psoriasis, arteriosclerosis, and cancer. Therapeutically, monoclonal antibodies targeting IL-6 (e.g. tocilizumab) or JAK inhibitors (e.g. ruxolitinib) have already been tested in other inflammatory diseases and may represent candidate agents for repurposing in adenomyosis models ( Taskin  et al. , 2016 ;  El-Zayadi  et al. , 2020 ;  Li  et al. , 2025 ;  Parisi  et al. , 2025 ). Furthermore, accumulating evidence shows that IL-6 can interact with WNT/β-catenin signaling. For example, hypoxia-induced IL-6 secretion can activate WNT signaling in rat mesenchymal stem cells (MSCs) through the JAK2/STAT3 signaling ( Mao  et al. , 2019 ;  Chen  et al. , 2024 ). Although we did not detect sustained  Stat3  mRNA elevation—likely due to negative feedback by SOCS3 ( Gao  et al. , 2018 )—the persistent  Il6  mRNA overexpression suggests that post-transcriptional activation of STAT3 or alternative pathways (e.g. MAPK) may underlie lesion progression in adenomyosis. Interestingly, both  Stat3  and  Il6r  mRNA levels were significantly reduced in ADM uteri at 1 month but returned to baseline by 3 months. This coordinated downregulation of receptor and downstream effector suggests a transient suppression of IL-6 signaling, potentially as a homeostatic brake against acute inflammatory activation. The latter normalization may reflect a loss of this regulation, allowing sustained IL-6 activity to drive chronic tissue remodeling. Although we did not directly assess regulatory T‐cell (Treg) populations—a limitation of our work—it is plausible that reduced STAT3 signaling also impairs Treg maintenance. Future studies should include FoxP3 +  cell quantification to determine whether Treg deficiency contributes to the skewing toward CD4 +  and CD8 +  effector phenotypes observed in our adenomyosis model.\nConcomitant with lL-6 upregulation, we detected a transient rise in  Il10  mRNA at 1 month, which normalized by 3 months. Similar patterns have been reported in chronic endometrial inflammation models, where initial IL-10 increases serve to limit tissue damage ( Yang  et al. , 2017 ;  Park  et al. , 2025 ). IL-10, produced primarily by M2 macrophages and regulatory T cells, suppresses pro-inflammatory cytokine secretion and downregulates antigen presentation ( Moore  et al. , 2001 ). In the context of adenomyosis, early IL-10 induction may represent a host attempt to counterbalance IL-6–driven inflammation and prevent excessive fibrosis. However, prolonged IL-10 signaling can also foster immunotolerance, facilitating lesion survival by inhibiting cytotoxic responses ( Couper  et al. , 2008 ). Thus, the dynamic IL-6/IL-10 interplay we observe likely reflects a bidirectional push–pull between inflammation and regulation that shapes disease outcome. Notably, the sustained IL-6 upregulation and shifts in CD3 +  T-cell subset composition observed here could serve as candidate biomarkers for disease staging or monitoring if validated in patient samples. Their detection in endometrial biopsies or peripheral blood may provide minimally invasive tools for clinical stratification.\nIn contrast to  Il6  and  Il10 ,  Ptgs2  mRNA expression remained unchanged in ADM uteri at both 1 and 3 months. This finding diverges from a preclinical study using tamoxifen-induced adenomyosis in mice, where immunohistochemical analysis revealed high-level COX-2 protein expression in adenomyosis lesions, and pharmacological inhibition by selective COX-2 inhibitor celecoxib markedly reduced adenomyosis severity, including lesion depth, fibrosis, and epithelial–mesenchymal transition (EMT) markers ( Jin  et al. , 2020 ). The discrepancy may stem from methodological differences: our whole-uterus PCR approach likely dilutes localized expression seen in lesion-restricted IHC. These findings suggest that COX-2 may still play a functional role in adenomyosis progression, and protein-level analyses will be needed to clarify its contribution.\nOur current murine data both align with and expand recent work in adenomyosis. Previously, using the same murine model, we documented compromised reproductive capacity, thereby establishing a functional readout of lesion impact on fertility ( Squatrito  et al. , 2024 ). The immune alterations we now describe offer a mechanistic bridge: early M2 dominance and  Il10  rise may initially buffer tissue damage yet predispose to chronic inflammation, while later loss of M1‐like macrophages and skewed T-cell differentiation (linked to transient  Stat3  downregulation) could underlie the uterine dysfunction and subfertility observed in that study.\nThese findings resonate with other studies. Indeed, Bourdon  et al.  showed that infiltrating immune cells cooperate with Notch1 signaling to drive epithelial-to-mesenchymal transition during lesion development in mice ( Bourdon  et al. , 2021 ). Building on this, they also found that reduced fertility in the same murine adenomyosis model is associated with an altered uterine immune profile at the implantation period ( Bourdon  et al. , 2024 ). Disrupted uterine NK-cell distribution and altered macrophage phenotypes have also been associated with lower pregnancy success in adenomyosis patients undergoing ART ( Bourdon  et al. , 2025 ). A recent study also evidenced that M2 macrophages accumulate within human adenomyotic lesions and actively promote endometrial cell invasiveness ( Stratopoulou  et al. , 2023 ). Collectively, these murine and human studies support a model in which dynamic innate–adaptive immune remodeling not only drives lesion progression but also translates into the fertility impairments we first characterized in our reproductive‐outcome study ( Squatrito  et al. , 2024 ). Whether these immune and structural changes translate into altered uterine contractility, receptivity, or implantation dynamics remains to be functionally addressed. Future  in vivo  or  ex vivo  assays, such as uterine peristalsis tracking or embryo transfer experiments, could help establish causality between immune remodeling and reproductive dysfunction.\nA major strength of our study is the use of a well‐validated murine adenomyosis model ( Parrott  et al. , 2001 ;  Marquardt  et al. , 2020 ) that isolates the effects of adenomyotic lesions from other uterine pathologies such as endometriosis or fibroids. We have characterized, for the first time, time‐dependent immune remodeling in the uterus, including shifts in macrophage polarization, T‐cell subsets, and cytokine expression.\nNevertheless, several limitations should be acknowledged. First, species differences and the simplified nature of the model may limit direct extrapolation to human adenomyosis, which often presents with heterogeneous lesions and coexists with endometriosis. Second, although mice were synchronized with β-estradiol, incomplete responses in some controls and disrupted cyclicity in ADM mice may have introduced residual variability, despite uniform synchronization across groups. Moreover, our analysis was restricted to two time points (1 and 3 months), which capture pre-lesional and established phases but do not resolve intermediate disease dynamics.\nTechnical factors should also be considered. Gene expression was assessed on whole uterine horns, which may dilute lesion-specific signals, and flow cytometry required pooling of uteri prior to histological confirmation, precluding one-to-one correlation with lesion grade. Furthermore, molecular analyses were limited to mRNA; protein-level validation and functional readouts remain to be performed.\nFinally, our immune profiling was limited in resolution. Flow cytometry did not assess T‐cell activation markers (e.g. FoxP3, CD69) or cytokine production at the protein level, which may overlook important functional diversity. A finer characterization of macrophage polarization states (e.g. M2a, M2b, M2c) and CD4 +  T-helper subtypes (e.g. Th1, Th2, Th17, Treg), as well as the inclusion of additional cytokines such as TNF-α, IFN-γ, IL-17, and TGF-β, will be essential to provide a more comprehensive picture of immune remodeling in adenomyosis.\n\nIn conclusion, adenomyosis induction in mice provokes a stepwise immune remodeling, beginning with M2 macrophage dominance and culminating in adaptive T‐cell reprogramming underpinned by cytokine signaling shifts. These results identify immune alterations that may represent candidate markers for future investigation. Although intriguing, the translational relevance of these findings remains to be confirmed, and targeting IL6/STAT3 signaling should be considered a hypothesis-generating observation that requires further validation. Translating these findings to patients could enable immune‐based stratification and personalized interventions aimed at restoring uterine immune homeostasis.","source_license":"CC0","license_restricted":false}