{"paper_id":"e75b2e6d-cf2f-4880-8cd2-bf7aa64a1b5f","body_text":"Abstract\nNADPH oxidase 4 (NOX4) plays an important role in the regulation of oxidative stress, which is associated with endometriosis. This study aims to investigate the effects of NOX4 in endometriosis and its molecular mechanisms. Clinical specimens were collected, and human endometrial stromal cells (HESCs) were isolated. The knockdown of NOX4 cell lines was established on the HESCs and induced by peritoneal fluid. The levels of NOX4 were determined by using immunohistochemistry (IHC) staining, western blotting, and qPCR, respectively. The levels of oxidative stress markers were determined by using western blotting and ELISAs, respectively. The correlation of NOX4 and oxidative stress markers was analyzed by the Pearson correlation coefficient. The levels of NOX4 were dramatically elevated in the ectopic endometrium. Besides, oxidative stress biomarkers were also dysregulated in the ectopic endometrium as compared to the normal endometrium. Pearson’s correlation coefficient analysis revealed a relationship between NOX4 and oxidative stress biomarkers in the ectopic endometrium. NOX4 modulated the expressions of oxidative stress markers in endometrial stromal cells stimulated by the peritoneal fluid from endometriosis. The effects of NOX4 on endometriosis are in part by its regulatory effects against oxidative stress.\nSimilar content being viewed by others\nData availability\nThe raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.\nReferences\nAmreen S, Kumar P, Gupta P, Rao P (2019) Evaluation of oxidative stress and severity of endometriosis. 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The study was performed in strict accordance with the Declaration of Helsinki, Ethical Principles for Medical Research Involving Human Subjects.\nConsent for publication\nCurrent study is available from the corresponding author on reasonable request.\nConflict of interest statement\nThe authors declare no competing interests.\nAdditional information\nCommunicated by Ewa Ziętkiewicz\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nSpringer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.\nAbout this article\nCite this article\nWang, X., Jiang, X., Lv, X. et al. NADPH oxidase 4-mediating oxidative stress contributes to endometriosis. J Appl Genetics 65, 113–120 (2024). https://doi.org/10.1007/s13353-023-00810-7\nReceived:\nRevised:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s13353-023-00810-7","source_license":"public-domain-us","license_restricted":false}