{"paper_id":"e74146a4-68ce-4af3-b424-d355c0f9e88b","body_text":"Abstract\nPurpose\nThe aim of the present study was to analyze the lipid profile of follicular fluid from patients with endometriosis and endometrioma who underwent in vitro fertilization treatment (IVF).\nMethods\nThe control group (n = 10) was composed of women with tubal factor or minimal male factor infertility who had positive pregnancy outcomes after IVF. The endometriosis group consisted of women with endometriosis diagnosed by videolaparoscopy (n = 10), and from the same patients, the endometriomas fluids were collected, which composed the endometrioma group (n = 10). From the follicular fluid and endometriomas, lipids were extracted by the Bligh and Dyer method, and the samples were analyzed by tandem mass spectrometry.\nResults\nWe observed phosphatidylglycerol phosphate, phosphatidylcholine, phosphatidylserine, and phosphatidylnositol bisphosphate in the control group. In the endometriosis group, sphingolipids and phosphatidylcholines were more abundant, while in the endometrioma group, sphingolipids and phosphatidylcholines with different m/z from the endometriosis group were found in high abundance.\nConclusion\nThis analysis demonstrated that there is a differential representation of these lipids according to their respective groups. In addition, the lipids found are involved in important mechanisms related to endometriosis progress in the ovary. Thus, the metabolomic approach for the study of lipids may be helpful in potential biomarker discovery.\nSimilar content being viewed by others\nReferences\nViganò P, Parazzini F, Somigliana E, Vercellini P. Endometriosis: epidemiology and aetiological factors. Best Pract Res Clin Obstet Gynaecol. 2004;18:177.\nBulun SE. Endometriosis. N Engl J Med. 2009;360:268–79.\nBektaş H, Bilsel Y, Sari YS, Ersöz F, Koç O, Deniz M, et al. 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Biochem Biophys Res Commun. 2007;358:196–202.\nAcknowledgments\nThis work was supported by CNPq (National Council for Scientific and Technological Development), Brazil.\nConflict of interest\nAll authors declare that there are no conflicts of interest.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nAdditional information\nCapsule Lipid profiling of follicular fluid identifies catgories that may be involved in cell signaling during the progression of ovarian endometriosis.\nWork conducted: Sao Paulo Federal University – Sao Paulo Hospital, Sao Paulo, Brazil\nElectronic supplementary material\nBelow is the link to the electronic supplementary material.\nESM 1 (download DOC )\n(DOC 387 kb)\nRights and permissions\nAbout this article\nCite this article\nCordeiro, F.B., Cataldi, T.R., Perkel, K.J. et al. Lipidomics analysis of follicular fluid by ESI-MS reveals potential biomarkers for ovarian endometriosis. J Assist Reprod Genet 32, 1817–1825 (2015). https://doi.org/10.1007/s10815-015-0592-1\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s10815-015-0592-1","source_license":"CC0","license_restricted":false}