{"paper_id":"e48bd260-a3e0-480f-af67-e00b98f540e1","body_text":"Abstract\nPurpose\nMucin-1(MUC1), a type of glucoprotein, has various expression statuses on the endometrium at different menstrual phases. Normal expression of MCU1 is closely related to successful blastocyst implantation. MUC1 was synthesized and secreted by the glandular epithelium of the endometrium, which acts as a barrier to foreign bodies. The endometrium was able to recognize and accept the blastocyst implantation. Endometriosis has lower rates of successful implantation with in vitro fertilization and embryo transfer, which may involve the altered expression of MUC1 on eutopic endometrium. In vitro studies demonstrated that progesterone and its receptors affected MUC1 expression under different physiopathology conditions. Our study was designed to explore whether progesterone receptors alteration contributed to MUC1 varied expression on endometriosis endometrium.\nMethods\nA total of 54 patients with different infertility reason were selected retrospectively from the First Affiliated Hospital of Soochow University and Kowloon Hospital, Suzhou, China. Endometrium samples were collected under operative procedures. The samples were assigned to two groups: endometriosis (n = 28) and oviduct block (control group, n = 26), on which we examined the expression of progesterone receptor B (PR-B) and MUC1, respectively, using immunohistochemistry and Western blotting.\nResults\nCompared with the control group, the expression of MUC1 was significantly decreased in the mid-secretory phase of the menstrual cycle in endometriosis. There was relatively lower expression of MUC1 in different phases of the cycle in comparison with the control group. The varied expression of MUC1 was significantly related to PR-B variation in endometriosis endometrium (r = 0.763, P < 0.01).\nConclusion\nDecreased expression of MUC1 may attribute to PR-B variation in the mid-secretory phase of endometriosis endometrium. It might be one of the factors resulted in infertility in endometriosis patients.\nSimilar content being viewed by others\nReferences\nBrayman M, Thathiah A, Carson DD (2004) MUC1: a multifunctional cell surface component of reproductive tissue epithelia. Reprod Biol Endocrinol 7:4\nHey NA, Aplin JD (1996) Sialyl-Lewis x and Sialyl-Lewis a are associated with MUC1 in human endometrium. Glycoconj J 13(5):769–779\nGenbacev OD, Prakobphol A, Foulk RA, Krtolica AR, Ilic D, Singer MS et al (2003) Trophoblast l-selectin-mediated adhesion at the maternal-fetal interface. Science 299(5605):405–408\nHey NA, Graham RA, Seif MW, Aplin JD (1994) The polymorphic epithelial mucin MUC1 in human endometrium is regulated with maximal expression in the implantation phase. J Clin Endocrinol Metab 78(2):337–342\nAplin JD, Hey NA, Graham RA (1998) Human endometrial MUC1 carries keratan sulfate: characteristic glycoforms in the luminal epithelium at receptivity. Glycobiology 8(3):269–276\nHorne AW, White JO, Margara RA, Williams R, Winston RM, Lalani E (2001) MUC 1: a genetic susceptibility to infertility? Lancet 357(9265):1336–1337\nHorne AW, Lalani EN, Margara RA, White JO (2006) The effects of sex steroid hormones and interleukin-1-beta on MUC1 expression in endometrial epithelial cell lines. Reproduction 131(4):733–742\nBrayman MJ, Julian J, Mulac-Jericevic B, Conneely OM, Edwards DP, Carson DD (2006) Progesterone receptor isoforms A and B differentially regulate MUC1 expression in uterine epithelial cells. Mol Endocrinol 20(10):2278–2291\nMulac-Jericevic B, Mullinax RA, DeMayo FJ, Lydon JP, Conneely OM (2000) Subgroup of reproductive functions of progesterone mediated by progesterone receptor-B isoform. Science 289(5485):1751–1754\nSharpe-Timms KL (2001) Endometrial anomalies in women with endometriosis. Ann N Y Acad Sci 943:131–147\nBurney RO, Talbi S, Hamilton AE, Vo KC, Nyegaard M, Nezhat CR et al (2007) Gene expression analysis of endometrium reveals progesterone resistance and candidate susceptibility genes in women with endometriosis. Endocrinology 148(8):3814–3826\nSharkey AM, Smith SK (2003) The endometrium as a cause of implantation failure. Best Pract Res Clin Obstet Gynaecol 17(2):289–307\nArici A, Oral E, Bukulmez O, Duleba A, Olive DL, Jones EE (1996) The effect of endometriosis on implantation: results from the Yale University in vitro fertilization and embryo transfer program. Fertil Steril 65(3):603–607\nLedee-Bataille N, Lapree-Delage G, Taupin JL, Dubanchet S, Frydman R, Chaouat G (2002) Concentration of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation. Hum Reprod 17(1):213–218\nOsteen KG, Bruner-Tran KL, Eisenberg E (2005) Reduced progesterone action during endometrial maturation: a potential risk factor for the development of endometriosis. Fertil Steril 83(3):529–537\nBulun SE, Cheng YH, Yin P, Imir G, Utsunomiya H, Attar E et al (2006) Progesterone resistance in endometriosis: link to failure to metabolize estradiol. Mol Cell Endocrinol 248(1–2):94–103\nNoyes RW, Hertig AT, Rock J (1975) Dating the endometrial biopsy. Am J Obstet Gynecol 122(2):262–263\nThathiah A, Carson DD (2002) Mucins and blastocyst attachment. Rev Endocr Metab Disord 3(2):87–96\nHorne AW, White JO, el Lalani N, Mobberley MA, Margara RA, Trew GH et al (2002) Analysis of epitopes on endometrial epithelium by scanning immunoelectron microscopy. Biochem Biophys Res Commun 292(1):102–108\nDeLoia JA, Krasnow JS, Brekosky J, Babaknia A, Julian J, Carson DD (1998) Regional specialization of the cell membrane-associated, polymorphic mucin (MUC1) in human uterine epithelia. Hum Reprod 13(1O):2902–2909\nDockery P, Li TC, Rogers AW, Cooke ID, Lenton EA (1988) The ultrastructure of the glandular epithelium in the timed endometrial biopsy. Hum Reprod 3(7):826–834\nJackson KS, Brudney A, Hastings JM, Mavrogianis PA, Kim JJ, Fazleabas AT (2007) The altered distribution of the steroid hormone receptors and the chaperone immunophilin FKBP52 in a baboon model of endometriosis is associated with progesterone resistance during the window of uterine receptivity. Reprod Sci 14(2):137–150\nMeseguer M, Aplin JD, Caballero-Campo P, O’Connor JE, Martin JC, Remohi J et al (2001) Human endometrial mucin MUC1 is up-regulated by progesterone and down-regulated in vitro by the human blastocyst. Biol Reprod 64(2):590–601\nLanz RB, Chua SS, Barron N, Soder BM, DeMayo F, O’Malley BW (2003) Steroid receptor RNA activator stimulates proliferation as well as apoptosis in vivo. Mol Cell Biol 23(20):7163–7176\nGiangrande PH, McDonnell DP (1999) The A and B isoforms of the human progesterone receptor: two functionally different transcription factors encoded by a single gene. Recent Prog Horm Res 54:291–313 (discussion 313–314)\nAttia GR, Zeitoun K, Edwards D, Johns A, Carr BR, Bulun SE (2000) Progesterone receptor isoform A but not B is expressed in endometriosis. J Clin Endocrinol Metab 85(8):2897–2902\nIgarashi TM, Bruner-Tran KL, Yeaman GR, Lessey BA, Edwards DP, Eisenberg E et al (2005) Reduced expression of progesterone receptor-B in the endometrium of women with endometriosis and in cocultures of endometrial cells exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin. Fertil Steril 84(1):67–74\nKao LC, Germeyer A, Tulac S, Lobo S, Yang JP, Taylor RN et al (2003) Expression profiling of endometrium from women with endometriosis reveals candidate genes for disease-based implantation failure and infertility. Endocrinology 144(7):2870–2881\nWu Y, Strawn E, Basir Z, Halverson G, Guo SW (2006) Promoter hypermethylation of progesterone receptor isoform B (PR-B) in endometriosis. Epigenetics 1(2):106–111\nWu Y, Starzinski-Powitz A, Guo SW (2008) Prolonged stimulation with tumor necrosis factor-alpha induced partial methylation at PR-B promoter in immortalized epithelial-like endometriotic cells. Fertil Steril 90(1):234–237\nCho SH, Oh YJ, Nam A, Kim HY, Park JH, Kim JH et al (2007) Evaluation of serum and urinary angiogenic factors in patients with endometriosis. Am J Reprod Immunol 58(6):497–504\nRichter ON, Dorn C, Rosing B, Flaskamp C, Ulrich U (2005) Tumor necrosis factor alpha secretion by peritoneal macrophages in patients with endometriosis. Arch Gynecol Obstet 271(2):143–147\nThathiah A, Brayman M, Dharmaraj N, Julian JJ, Lagow EL, Carson DD (2004) Tumor necrosis factor alpha stimulates MUC1 synthesis and ectodomain release in a human uterine epithelial cell line. Endocrinology 145(9):4192–4203\nCunha-Filho JS, Gross JL, Bastos de Souza CA, Lemos NA, Giugliani C, Freitas F et al (2003) Physiopathological aspects of corpus luteum defect in infertile patients with mild/minimal endometriosis. J Assist Reprod Genet 20(3):117–121\nDharmaraj N, Wang P, Carson DD (2010) Cytokine and progesterone receptor interplay in the regulation of MUC1 gene expression. Mol Endocrinol 24(12):2253–2266\nAcknowledgments\nWe wish to acknowledge the assistance of Dr. Yuanyuan Ruan (Shanghai Medical College of FuDan University).\nConflict of interest\nWe declare that we have no conflict of interest.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nShen, F., Yan, C., Liu, M. et al. Decreased expression of mucin-1 in endometriosis endometrium correlated with progesterone receptor B involved in infertility. Arch Gynecol Obstet 291, 439–445 (2015). https://doi.org/10.1007/s00404-014-3419-x\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s00404-014-3419-x","source_license":"CC0","license_restricted":false}