{"paper_id":"e31b6dc2-d6bf-4c9d-b92b-5a39b0f81f4c","body_text":"Archives of Biological Sciences 2014 Volume 66, Issue 2, Pages: 735-741 Estrogen receptor β agonist inhibits proliferation of endometrial stromal cells in an estrogen-receptor independent manner Xia Qiong (Wuhan Puai Hospital, Department of Obstetrics, Gynecology, Wuhan, China) The finding that estrogen receptor ERβ agonists induce regression of ectopic\nendometrial implants in a rodent model has rekindled interest in the role of\nERβ in the growth and maintenance of human endometriosis. We hypothesize that\nthe ERβ agonist may have a direct effect on the human endometrium. We\nexamined the mRNA and protein expression of ERβ in both eutopic and ectopic\nendometrium and performed a luciferase reporter assay for the presence of\nfunctional ERβ in human endometrial stromal cells. Diarylpropionitrile (DPN -\na ERβ specific agonist) significantly inhibited endometrial stromal cell\nproliferation at 10 M by 25% but not at 10 nM. ERβ mRNA is present in both\neutopic and ectopic endometrium; however, protein expression is absent in\nboth stroma and epithelium. Our search for functional ERβ similarly showed an\nabsence in endometrial stromal cells. These results suggest that the\nregression of endometriosis might be ERβ-independent. Keywords: endometrium, neoplasm, pathology, estrogen, receptor\nhttps://doi.org/10.2298/ABS1402735X\nFull text ( 628 KB)\nZhao Dong (Tongji University School of Medicine, Shanghai First Maternity and Infant Hospital, Department of Obstetrics, Gynecology, Shanghai, China)\nWang Juan (Department of Obstetrics, Gynecology, Wuhan Puai Hospital, Wuhan, China)","source_license":"CC0","license_restricted":false}