{"paper_id":"e00d1c28-bc61-42b1-bfcb-df6abd91978f","body_text":"摘要目的 了解子宫内膜异位症患者中CD19+CD5+B细胞的变化,CD19+CD5+B细胞中BAFF(B淋巴细胞刺激因子)的表达和凋亡敏感性变化,探讨CD19+CD5+B细胞与子宫内膜异位症的关系.方法 39例子宫内膜异位症患者为实验组,31例子宫肌瘤患者为对照组,流式细胞术检测患者外周血、腹腔冲洗液以及子宫内膜组织中CD19+CD5+B细胞的变化,Q-PCR检测CD19+CD5+B细胞中BAFF mRNA的表达,流式细胞术PI-Annexin V检测CD19+CD5+B细胞对CD95L诱导的凋亡敏感性.结果 实验组患者外周血、腹腔冲洗液以及异位子宫内膜组织中CD19+CD5+B细胞高于对照组(P＜0.01);实验组患者各样本中CD19+CD5+B细胞的BAFF mRNA表达水平高于对照组,CD19+CD5+B细胞能明显抵抗CD95L所诱导的凋亡(P＜0.01).结论 在子宫内膜异位症中,CD19+CD5+B细胞高表达BAFF,高对抗CD95L诱导的凋亡,参与疾病的发生.\n更多相关知识\nabstractsObjective To explore the changes of frequency, BAFF expression and sensitivity to apoptosis of CD19 + CD5 + B cells in endometriosis patients and investigate the relationship between CD19 +CD5 + B cells and endometriosis. Methods Flow cytometry and quantitative PCR were used to assess the frequency, BAFF expression and sensitivity to apoptosis of CD19+ CD5 + B cells in the peripheral blood,peritoneal fluids and endometrium biopsies of 39 patients with endometriosis and 31 patients with myoma of uterus ( control subjects). Results: All 39 endometriosis patients had higher frequencies ( all P ＜ 0. 01 ) of CD19 + CD5 + B cells in the peripheral blood, peritoneal fluids and endometrium biopsies than that in 31 control subjects. CD19 + CD5 + B cells from endometriosis patients expressed higher BAFF, and were more resistant to CD95L-induced apoptosis than the cells from control subjects ( P ＜ 0. 01 ). Conclusion CD19 + CD5 + B cells might play an important role in the pathogenesis of endometriosis through higher BAFF expression and more resistance to CD95L-induced apoptosis.\nMore相关知识\n- 浏览386\n- 被引0\n- 下载16\n相似文献\n- 中文期刊\n- 外文期刊\n- 学位论文\n- 会议论文","source_license":"CC0","license_restricted":false}