{"paper_id":"df385d0e-8e6f-4148-b9c5-65ad169b45aa","body_text":"Abstract\nThe aim of this study was to evaluate and compare the mitogenic effect of peritoneal fluid (PF) from women with mild and severe endometriosis on the endometrial stromal cell proliferation. Increasing concentrations of PF from women with and without mild or severe endometriosis were added to primary endometrial stromal cell cultures and3H-thymidine incorporation was used to assess DNA synthesis in these cultures. PF from women with mild endometriosis induced a statistically significant dose-dependent increase in stromal cell thymidine uptake ranged from 5.8 to 14.5 fold, whereas PF from women with severe endometriosis produced an average 51% inhibition of stromal cell proliferation of compared with cells exposed to non-endometriosis PF or exposed to nutrient medium supplemented with 2.5% calf serum alone. PF samples from patients with stage I endometriosis induced a statistically dose-dependent increase in stromal cell proliferation, whereas PF from patients with stage IV endometriosis caused a significant inhibition.\nSimilar content being viewed by others\nReferences\nAmerican Fertility Society (1985) Revised American fertility society classification of endometriosis. Fertil Steril 43:351–352\nBatzofin JH, Holmes SD, Gibbons WE, Buttram VC (1985) Peritoneal fluid plasminogen activator activity in endometriosis and pelvic adhesive disease. Fertil Steril 44:277–279\nCarpenter G, Cohen S (1979) Epidermal growth factor. Annu Rev Biochem 48:193–216\nDe Leon FD, Vijayakumar R, Brown M, Rao CH, Yussman MA, Schultz G (1986) Peritoneal fluid volume, estrogen, prostaglandin, and epidermal growth factor concentrations in patients with and without endometriosis. Obstet Gynecol 68:189–194\nDrake T, O’Brian W, Grunet G (1980) Peritoneal fluid volume in endometriosis. 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Obstet Gynecol 80:405–409\nAuthor information\nAuthors and Affiliations\nRights and permissions\nAbout this article\nCite this article\nMeresman, G.F., Barañao, R.I., Tenenbaum, A. et al. Effect of peritoneal fluid from patients with mild and severe endometriosis on endometrial stromal cell proliferation. Arch Gynecol Obstet 259, 109–115 (1997). https://doi.org/10.1007/BF02505318\nReceived:\nAccepted:\nIssue date:\nDOI: https://doi.org/10.1007/BF02505318","source_license":"CC0","license_restricted":false}