{"paper_id":"dd88e5fb-9b23-47af-ace4-25a37a8a9ed0","body_text":"Abstract\nEctopic endometrial tissue induces various reactions in surrounding tissues, such as the surface of the ovary and peritoneal cavity, leading to endometriosis. The aim of this study is to investigate the expression profile of extracellular matrix (ECM) and adhesion molecules in the early steps of development of experimental mouse endometriosis, specifically in peritoneum adjacent to endometrium transplants attached via autotransplantation. The endometriosis model was induced by autotransplantation of endometrium to peritoneal tissue. Peritoneal tissues adjacent to the transplant were obtained at 1, 4, and 7 days posttransplantation. The results showed that messenger RNA expression levels of most of the integrins, collagens, and other ECM reached a peak at 7 days posttransplantation. Uniquely, Lamc2 was significantly increased to its maximum level within 24 hours posttransplantation and may be strongly associated with initiation of the development of endometriosis. These data will be helpful in further investigations of the treatment of endometriosis.\nSimilar content being viewed by others\nReferences\nGiudice LC, Kao LC. Endometriosis. Lancet. 2004;364(9447): 1789–1799.\nUchiide I, Ihara T, Sugamata M. Pathological evaluation of the rat endometriosis model. Fertil Steril. 2002;78(4):782–786.\nUmezawa M, Sakata C, Tanaka N, et al. Cytokine and chemokine expression in a rat endometriosis is similar to that in human endometriosis. Cytokine. 2008;43(2):105–109.\nFlores I, Rivera E, Ruiz LA, Santiago OI, Vernon MW, Appleyard CB. Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease. Fertil Steril. 2007;87(5):1180–1199.\nVernon MW, Wilson EA. Studies on the surgical induction of endometriosis in the rat. Fertil Steril. 1985;44(5):684–694.\nSugamata M, Ihara T, Uchiide I. Increase of activated mast cells in human endometriosis. Am J Reprod Immunol. 2005;53(3): 120–125.\nUmezawa M, Tanaka N, Tainaka H, Takeda K, Ihara T, Sugamata M. Microarray analysis provides insight into early steps of pathophysiology of mouse endometriosis model induced by autotransplantation of endometrium. Life Sci. 2009;84(23–24):832–837.\nStilley JA, Birt JA, Nagel SC, Sutovsky M, Sutovsky P, Sharpe-Timms KL. Neutralizing TIMP1 restores fecundity in a rat model of endometriosis and treating control rats with TIMP1 causes anomalies in ovarian function and embryo development. Biol Reprod. 2010;83(2):185–194.\nLee B, Du H, Taylor HS. Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium. Biol Reprod. 2009;80(1):79–85.\nUmezawa M, Takeda K, Ihara T, Sugamata M. Novel insights into the pathogenesis of endometriosis from a disease model induced by autotransplantation of endometrium. Inflamm Regen. 2010; 30:115–119.\nRegidor PA, Vogel C, Regidor M, Schindler AE, Winterhager E. Expression pattern of integrin adhesion molecules in endometriosis and human endometrium. Hum Reprod Update. 1998;4(5): 710–718.\nKlemmt PA, Carver JG, Koninckx P, McVeigh EJ, Mardon HJ. Endometrial cells from women with endometriosis have increased adhesion and proliferative capacity in response to extracellular matrix components: towards a mechanistic model for endometriosis progression. Hum Reprod. 2007;22(12):3139–3147.\nSampson JA. Peritoneal endometriosis due to the menstrual dissemination of endometrial tissue into the peritoneal cavity. Am J Obstet Gynecol. 1927;14:422–469.\nNetsu S, Konno R, Odagiri K, Soma M, Fujiwara H, Suzuki M. Oral eicosapentaenoic acid supplementation as possible therapy for endometriosis. Fertil Steril. 2008;90(suppl 4):1496–1502.\nMachado DE, Berardo PT, Palmero CY, Nasciutti LE. Higher expression of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) and metalloproteinase-9 (MMP-9) in a rat model of peritoneal endometriosis is similar to cancer diseases. J Exp Clin Cancer Res. 2010;29:4.\nPirilä E, Sharabi A, Salo T, et al. Matrix metalloproteinases process the laminin-5 gamma 2-chain and regulate epithelial cell migration. Biochem Biophys Res Commun. 2003;303(4): 1012–1017.\nGu Z, Cui J, Brown S, et al. A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia. J Neurosci. 2005;25(27):6401–6408.\nSabeh F, Li XY, Saunders TL, Rowe RG, Weiss SJ. Secreted versus membrane-anchored collagenases: relative roles in fibroblastdependent collagenolysis and invasion. J Biol Chem. 2009; 284(34):23001–23011.\nKyama CM, Debrock S, Mwenda JM, D’Hooghe TM. Potential involvement of the immune system in the development of endometriosis. Reprod Biol Endocrinol. 2003;1:123.\nUmezawa M, Takeda K, Ihara T, Sugamata M. Advantages and disadvantages of each animal model of endometriosis. In: Mitchell LA, ed. Endometriosis: Symptoms, Diagnosis and Treatments. New York, NY: Nova Science; 2011:261–263.\nWitz CA, Takahashi A, Montoya-Rodriguez IA, Cho S, Schenken RS. Expression of the alpha2beta1 and alpha3beta1 integrins at the surface of mesothelial cells: a potential attachment site of endometrial cells. Fertil Steril. 2000;74(3):579–584.\nWitz CA. Cell adhesion molecules and endometriosis. Semin Reprod Med. 2003;21(2):173–182.\nvan der Rest M, Garrone R. Collagen family of proteins. FASEB J. 1991;5(13):2814–2823.\nUmezawa M, Sakata C, Tabata M, et al. Diesel exhaust exposure enhances the persistence of endometriosis model in rats. J Health Sci. 2008;54:503–507.\nTimpl R, Rohde H, Robey PG, Rennard SI, Foidart JM, Martin GR. Laminin-a glycoprotein from basement membranes. J Biol Chem. 1979;254(19):9933–9937.\nKoks CA, Groothuis PG, Dunselman GA, de Goeij AF, Evers JL. Adhesion of menstrual endometrium to extracellular matrix: the possible role of integrin alpha(6)beta(1) and laminin interaction. Mol Hum Reprod. 2000;6(2):170–177.\nMizushima H, Koshikawa N, Moriyama K, et al. Wide distribution of laminin-5 gamma 2 chain in basement membranes of various human tissues. Horm Res. 1998;50(suppl 2):7–14.\nChang YC, Sabourin CL, Lu SE, et al. Upregulation of gamma-2 laminin-332 in the mouse ear vesicant wound model. J Biochem Mol Toxicol. 2009;23(3):172–184.\nGuess CM, Quaranta V. Defining the role of laminin-332 in carcinoma. Matrix Biol. 2009;28(8):445–455.\nBéliard A, Donnez J, Nisolle M, Foidart JM. Localization of laminin, fibronectin, E-cadherin, and integrins in endometrium and endometriosis. Fertil Steril. 1997;67:266–272.\nJuliano RL, Haskill S. Signal transduction from the extracellular matrix. J Cell Biol. 1993;120(3):577–585.\nSelam B, Kayisli UA, Garcia-Velasco JA, Arici A. Extracellular matrix-dependent regulation of Fas ligand expression in human endometrial stromal cells. Biol Reprod. 2002;66(1):1–5.\nOkigami H, Takeshita K, Tajimi M, et al. Inhibition of eosinophilia in vivo by a small molecule inhibitor of very late antigen (VLA)-4. Eur J Pharmacol. 2007;559(2–3):202–209.\nvan Kaam KJ, Romano A, Dunselman GA, Groothuis PG. Transforming growth factor beta1 gene polymorphism 509C/T in deep infiltrating endometriosis. Reprod Sci. 2007;14(4):367–373.\nKang S, Li SZ, Wang N, et al. Association between genetic polymorphisms in fibroblast growth factor (FGF)1 and FGF2 and risk of endometriosis and adenomyosis in Chinese women. Hum Reprod. 2010;25(7):1806–1811.\nUmezawa M, Sakata C, Tanaka N, et al. Pathological study for the effects of in utero and postnatal exposure to diesel exhaust on a rat endometriosis model. J Toxicol Sci. 2011;36(4):493–498.\nIhara T, Uchiide I, Sugamata M. Light and electron microscopic evaluation of antileukotriene therapy for experimental rat endometriosis. Fertil Steril. 2004;81(suppl 1):819–823.\nGüney M, Nasir S, Oral B, Karahan N, Mungan T. Effect of caffeic acid phenethyl ester on the regression of endometrial explants in an experimental rat model. Reprod Sci. 2007;14(3):270–279.\nGüney M, Oral B, Karahan N, Mungan T. Regression of endometrial explants in a rat model of endometriosis treated with melatonin. Fertil Steril. 2008;89(4):934–942.\nZhang Y, Cao H, Hu YY, Wang H, Zhang CJ. Inhibitory effect of curcumin on angiogenesis in ectopic endometrium of rats with experimental endometriosis. Int J Mol Med. 2011;27(1):87–94.\nZulfikaroglu E, Kılıc S, Islimye M, Aydin M, Zergeroglu S, Batioglu S. Efficacy of anti-tumor necrosis factor therapy on endometriosis in an experimental rat model. Arch Gynecol Obstet. 2011;283(4):799–804.\nUmezawa M, Tanaka N, Takeda K, Ihara T, Sugamata M. Clarithromycin and telithromycin increases interleukin-10 expression in the rat endometriosis model. Cytokine. 2011;55(3):339–342.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nUmezawa, M., Saito, Y., Tanaka-Hattori, N. et al. Expression Profile of Extracellular Matrix and Adhesion Molecules in the Development of Endometriosis in a Mouse Model. Reprod. Sci. 19, 1365–1372 (2012). https://doi.org/10.1177/1933719112450340\nPublished:\nIssue date:\nDOI: https://doi.org/10.1177/1933719112450340","source_license":"public-domain-us","license_restricted":false}