{"paper_id":"dbf05b6a-83b3-42a6-8dc6-53740d565acc","body_text":"Dataset supporting the University of Southampton Doctoral Thesis \"Endometriosis – It’s Influence on Obstetric and Birth Outcomes and the Potential Role of Reactive Oxygen Species\".\nDataset supporting the University of Southampton Doctoral Thesis \"Endometriosis – It’s Influence on Obstetric and Birth Outcomes and the Potential Role of Reactive Oxygen Species\".\nDataset supporting the University of Southampton Doctoral Thesis \"Endometriosis – It’s influence on obstetric and birth outcomes and the potential role of reactive oxygen species\".\nThe data includes total antioxidant capacity (TAC) assay results, Thiobarbituric acid reactive substances (TBARS) assay results and lumigen total reactive oxygen species (ROS) assay results. These assays were applied to samples of peritoneal fluid, urine and serum of women with and without endometriosis taken at surgery and serum and urine obtained at follow up as part of thesis \"Endometriosis – It’s Influence on\nObstetric and Birth Outcomes and the Potential Role of Reactive Oxygen Species\"\nResults of intracellular redox status experiments. Microinjection of mouse oocytes with roGFP2 mRNA and measured fluorescence (mean integrated density of confocal images) of the oocytes at emission wavelength 530 nm following excitation at 405 nm and 488 nm wavelengths to measure the oxidation/reduction ratio of each oocyte when incubated in peritoneal fluid of women with and without endometriosis and control fluids.\nResults of experiments incubating mouse oocytes in peritoneal fluid (of women with and without endometriosis) and control fluids and performing immunofluorescence with Anti-8-OHG antibody. Mean fluorescence of anti-8-OHG was measured by integrated density in pixels.\nData is available only 'on request' to bone fide researchers with ethical approval. Please complete the attached request form and return it to researchdata@soton.ac.uk\nRelated projects/Funders:\nFunded by the Clinical Obstetric and Gynaecology division of the Human Development and Health department at the University of Southampton\nUniversity of Southampton\nHorton, Joanne Marie\n63f3d1c0-8257-4417-b0b8-eb120094680c\nCheong, Ying\n4efbba2a-3036-4dce-82f1-8b4017952c83\nLane, Simon\n8e80111f-5012-4950-a228-dfb8fb9df52d\nNg, Ka Ying Bonnie\n869b3b2d-dd7c-407d-88aa-5db399e02b3a\nUpsani, Gayatri\n0e73dbd9-6b6f-43e9-b012-a9358083f31c\nHorton, Joanne Marie\n63f3d1c0-8257-4417-b0b8-eb120094680c\nCheong, Ying\n4efbba2a-3036-4dce-82f1-8b4017952c83\nLane, Simon\n8e80111f-5012-4950-a228-dfb8fb9df52d\nNg, Ka Ying Bonnie\n869b3b2d-dd7c-407d-88aa-5db399e02b3a\nUpsani, Gayatri\n0e73dbd9-6b6f-43e9-b012-a9358083f31c\nHorton, Joanne Marie\n(2024)\nDataset supporting the University of Southampton Doctoral Thesis \"Endometriosis – It’s Influence on Obstetric and Birth Outcomes and the Potential Role of Reactive Oxygen Species\".\nUniversity of Southampton\ndoi:10.5258/SOTON/D3025\n[Dataset]\nAbstract\nDataset supporting the University of Southampton Doctoral Thesis \"Endometriosis – It’s influence on obstetric and birth outcomes and the potential role of reactive oxygen species\".\nThe data includes total antioxidant capacity (TAC) assay results, Thiobarbituric acid reactive substances (TBARS) assay results and lumigen total reactive oxygen species (ROS) assay results. These assays were applied to samples of peritoneal fluid, urine and serum of women with and without endometriosis taken at surgery and serum and urine obtained at follow up as part of thesis \"Endometriosis – It’s Influence on\nObstetric and Birth Outcomes and the Potential Role of Reactive Oxygen Species\"\nResults of intracellular redox status experiments. Microinjection of mouse oocytes with roGFP2 mRNA and measured fluorescence (mean integrated density of confocal images) of the oocytes at emission wavelength 530 nm following excitation at 405 nm and 488 nm wavelengths to measure the oxidation/reduction ratio of each oocyte when incubated in peritoneal fluid of women with and without endometriosis and control fluids.\nResults of experiments incubating mouse oocytes in peritoneal fluid (of women with and without endometriosis) and control fluids and performing immunofluorescence with Anti-8-OHG antibody. Mean fluorescence of anti-8-OHG was measured by integrated density in pixels.\nData is available only 'on request' to bone fide researchers with ethical approval. Please complete the attached request form and return it to researchdata@soton.ac.uk\nRelated projects/Funders:\nFunded by the Clinical Obstetric and Gynaecology division of the Human Development and Health department at the University of Southampton\nText\nD3025_readme.txt\n- Dataset\nText\nRequest-Form-Access-Ethics.docx\n- Dataset\nMore information\nPublished date: 2024\nIdentifiers\nLocal EPrints ID: 489102\nURI: http://eprints.soton.ac.uk/id/eprint/489102\nPURE UUID: d23f2a99-1113-4040-8827-1448e7ef3dcb\nCatalogue record\nDate deposited: 15 Apr 2024 16:33\nLast modified: 06 Mar 2026 02:59\nExport record\nAltmetrics\nContributors\nCreator:\nJoanne Marie Horton\nResearch team head:\nSimon Lane\nContributor:\nKa Ying Bonnie Ng\nContributor:\nGayatri Upsani\nDownload statistics\nDownloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.\nView more statistics","source_license":"CC0","license_restricted":false}