{"paper_id":"d4b97c1e-4e3c-4434-8d6c-52582a0a2603","body_text":"The Alzheimer Disease (AD) is a progressive and irreversible loss of neurons commonly characterized by a gradual decline of memory, language, and cognitive ability ( Kumar, Shankar, Reddy, Kumar, & Sumalatha, 2014 ). Accumulation of Amyloid β (Aβ) in the brain is considered as 1 of the major contributing factors to the development of AD ( Citron et al., 1997 ). Nabeshima and Nitta reported that intraventricular administration of Aβ in the rats resulted in learning and memory impairment accompanied by a decrease in choline acetyltransferase activity, suggesting that accumulation of Aβ is related to cognitive deficiency in AD ( Nabeshima & Nitta, 1994 ). Administration of Aβ leads to changes in Long-Term Potentiation (LTP) in the hippocampus and, consequently, impairs cognition and memory in rodents ( Shankar et al., 2008 ). Stress oxidative, protein oxidation, Reactive Oxygen Species (ROS) formation, and subsequent neuronal death are reported following Aβ deposition generated by an imbalance between ROS and internal antioxidants. Evidence show that appropriate nourishing with external antioxidants could improve brain damage and cognitive function ( Khodamoradi, Komaki, Salehi, Shahidi, & Sarihi, 2015 ;  Nezhadi et al., 2011 ).\nFurthermore, the external antioxidants can prevent the detrimental consequences of Aβ and are considered as a promoting approach to neuroprotection in the AD brain ( Ghahremanitamadon et al., 2014 ;  Zargooshnia et al., 2014 ).  Cyperus rotundus , as a species of sedge and traditional medicine are widely used worldwide to treat stomach ailments and wounds ( Puratchikody, Devi, & Nagalakshmi, 2006 ;  Uddin, Mondal, Shilpi, & Rahman, 2006 ).\nIt is shown that the major chemical components of  C. rotundus  are essential oils, flavonoids, terpenoids, mono and sesquiterpenes ( Singh, Kulshrestha, Gupta, & Bhargava, 1970 ;  Sonwa & Knig, 2001 ). Also, it is shown that hydroalcoholic extract of  C. rotundus  exhibited powerful free radical scavenging, especially against  1,1-diphenyl-2-picryl-hydrazyl (DPPH)  and superoxide anions, as well as a moderate effect on nitric oxide (Yazdanparast &  Ardestani, 2007 ). This extract showed an inhibitory effect against lipid peroxidation, protein oxidation, and glycoxidation ( Bahramikia, Ardestani, & Yazdanparast, 2009 ;  Kilani et al., 2008 ). It appears that  C. rotundus  contains potent components such as flavonoids that may be potentially useful to modulate the immune cell functions, provoking analgesics, and defending against inflammation and stress oxidative ( Dhillon, Singh, Kundra, & Basra, 1993 ). Malekian and Ghannadi showed that administration of  C. rotundus  improved the scopolamine-induced learning and memory deficit in mice ( Malekian, Rabbani, & Ghannadi, 2012 ). Due to the common usage of traditional medicine and natural antioxidants, the current study aims at investigating the ameliorating effects of  C. rotundus  extracts on Aβ (1-40)-induced amnesia.\n\nThe Aβ (1–40) was purchased from sigma-Aldrich company (St. Louis, MO, USA). Aβ 1-40 was solubilized in sterile water at 1 μg/μL concentration and stored at −20°C until use.\nAdult male Wistar rats (Pasteur Institute, Tehran, Iran), weighed 250 to 300 g were used in the study. The rats were accommodated the animal house in a 12:12 hours light/dark cycle (light on, 7:00 AM; light off, 7:00 PM) with free access to food and water. A week before the experimental procedure, the animals were habituated to their new environment. The guidelines of the National Institute of Health Guide for Care and Use of Laboratory Animals were performed for all experiments, and approved by the Veterinary Ethics Committee of the Iran University of Medical Sciences, Tehran, Iran.\nThe animals were randomly classified into the following groups (n=7 each group): The control group which was left undisrupted; and The sham-operated group. The Aβ model group received single bilateral intrahippocampal (IHP) injections of 6 μg Aβ 1-40 ( Zargooshnia et al., 2014 ). The  Cyperus rotundus -treated group received intraperitoneal injection of  C. rotundus  extract (400 mg/kg) following IHP injection of Aβ 1-40 for 14 days ( Hsieh, Peng, Wu, & Wang, 2000 ).\nIn June 2014, the dried  C. rotundus , with herbarium code TARI 12569, was collected from Iranian Institute of Medicinal Plant Field and grounded into coarse powder by electrically driven device and was soaked into aqueous ethanol (80%) for 1 week. It was passed through a Whatman filter paper (a cellulose filter to specify and recognize the materials in the qualitative analytical techniques) and vaporized by a rotary evaporator under the reduced pressure at a maximum of 40°C.The extract was completely dissolved in distilled water and kept at 4°C ( Malekianet et al., 2012 ).\nThe previously established method of stereotaxic surgery was used ( Zargooshnia et al., 2014 ) In brief, rats were anesthetized by xylazine (10 mg/kg) and ketamine (100 mg/kg), and placed into a stereotaxic device (Stoelting, USA). After retraction of scalp, the area surrounding bregma was cleaned and dried. Relative to the bregma and with the stereotaxic arm at 0°, the coordinates for the dentate gyrus were anterior-posterior (AP): 3.6 mm from bregma; Mediolateral (ML): +2.3 mm from mid-line; Dorsoventral (DV): 3 mm from skull surface ( Figure 1 ). Aβ solution (6 μL) was bilaterally injected into the region using a 10 μL microsyringe (Hamilton-Reno, NV, USA). Sham operated rats received vehicle solution.\nSchematic photograph representing the microinjection site of Amyloid β into the hippocampus (black arrow).\nMorris Water-Maze (MWM) is a behavioral task to assess hippocampal-related learning, including acquisition and retention of spatial memory and plays an important role in the validation of rodent models for neurocognitive disorders such as the Alzheimer disease ( D’Hooge & De Deyn, 2001 ;  Morris, Garrud, Rawlins, & O’Keefe, 1982 ). Therefore, the protocol used in the current investigation was derived from the previously performed study  Soleimani Asl et al. (2013) . In brief, a black circular pool filled with water (22±1°C) with an invisible plexiglass platform (located 1 cm below the water) was used. Some constant visual cues such as table, library, and computer were located around the MWM room.\nThe North, East, South, and West locations were selected to start the training trials. The animals were trained for 3 consecutive days at the same time (10:00 to 12:00 AM). Training included 2 blocks with 4 trials. There was a 5-minute rest between consecutive blocks. During trials, from each of the starting positions, the animals swam up the located hidden platform. The animals were allowed to spend 30 seconds on the platform between the 2 trials. There was a video camera above the pool that recorded escape latency (the time taken to reach the hidden platform) and traveled distance (the length of the swim path). On day 4, a probe trial was performed in which the platform was removed from the pool and each rat was allowed to swim for 60 seconds and percentage of entrance into the target quadrant was recorded.\nData were expressed as mean±standard error of the mean (SEM) and processed by commercially available software SPSS version 16. Results were analyzed using repeated measure and 2 way analysis of variance (treatment and training days as the factors). The Turkey multiple comparison test was used to analyze the significance of the differences between the groups, when appropriate. P<0.05 was considered statistically significant.\n\nEffects of  Cyperus rotundus  on the Aβ (1-40)-induced increase in escape latency in Morris water-maze: A 2-way analysis of variance of escape latency revealed significant effects of treatment [F(3, 4233)=10.63, P<0.001]. In addition, there was no significant interaction between treatment and training days. As shown in  Figure 2 , escape latency (the time to find the hidden platform) was less in the control group than the other groups. More time to find the hidden platform indicates more intense spatial memory impairment. A post hoc analysis of the 3 training days showed a significant difference between the control and sham-operated groups, and the rats that received Aβ (P<0.001). According to the current study results, the administration of  C. rotundus  caused significant reduction in escape latency compared with the Aβ-treated group (P<0.05).\nThe mean of latencies to find hidden platform in the MWM. Each block represents the average latency of three consecutive trial days. Data present as mean±S.E.M. (a: P<0.001 vs. control and sham groups; b: P<0.05 vs. Aβ-treated group).\nEffects of  Cyperus rotundus  on the Aβ (1-40)-induced increase in traveled distance in Morris water-maze: In accordance with the latency data, treatment had significant effect [F(3, 7080)=9.83, P<0.001]. There was no significant effect in both training days, and also no significant difference was observed in the interaction between training days and treatment. A significant difference in traveled distance was observed between Aβ-treated rats with the control and sham-operated groups (P<0.001) ( Figure 3 ). Aβ-treated rats that received  C. rotundus  extract for 7 days showed less traveled distance, in comparison with the Aβ-treated group (P<0.05).\nThe mean of traveled distance in the MWM. Each block represents the average of traveled distance of four consecutive trial days. Data present as mean±S.E.M. (a: P<0.001 vs. control and sham groups; b: P<0.05 vs. Aβ-treated group).\nEffects of  Cyperus rotundus  on the Aβ (1-40)-induced reduction in time spent in target quadrant in Morris water-maze: As shown in  Figure 4 , a 1-way analysis of variance (ANOVA) of time spent percent in the target quadrant showed that the control group spent more time in target quadrant (26.66±3.33) than the sham-operated (22.66±4.37), Aβ-treated (20±3.1) and  C. rotundus  (25.8±2.43).\nThe mean of the percent of time spent target quarter in the probe trial in the MWM. Data present as mean±S.E.M.\n\nThe current study aimed at evaluating the protective effects of  C. rotundus  extract on memory impairment following intrahippocampal injection of Aβ (1-40). The major findings of the current study were: (a) Intrahippocampal injection of Aβ (1-40) resulted in learning impairment; (b) Treatment with  C. rotundus  extract was protective against Aβ-induced memory impairment.\nPreviously published experimental studies reported that the infusion of Aβ(1–40) into the brain impaired one-trial/day reward learning ( Malin et al., 2001 ), and memory in radial-arm maze task confirmed the current study results ( Malin et al., 2001 ;  Tanaka et al., 1998 ). As memory impairment and degeneration of cholinergic neurons can be observed following the deposition of β-amyloid protein in the brain; it seems that the β-amyloid-treated rats could be used as the animal model for the Alzheimer disease (Nitta, Itoh, Hasegawa, &  Nabeshima, 1994 ).\nIt seems that due to the low antioxidant and cell membrane lipid, the brain is sensitive to oxidative stress ( Butterfield & Lauderback, 2002 ). Therefore, employment of external antioxidants, such as various spices and herbs, can be 1 of the popular remedial strategies to treat neurological diseases, and recovery of brain damage and cognitive deficiency ( Azad, Rasoolijazi, Joghataie, & Soleimani, 2011 ;  Ghahremanitamadon et al., 2014 ).\nCyperus rotundus  is a traditional medicinal plant used against stomach disorders and inflammatory bowel diseases ( Jagtap, Shirke, & Phadke, 2004 ;  Uddin et al. 2006 ).The current study demonstrated that administration of  C. rotundus  extract could improve Aβ-induced memory impairment. The protective effect of  C. rotundus  extract on memory can be related to its function of scavenging free radicals (Yazdanparast &  Ardestani, 2007 ).\nArdestani et al., showed the preventing effects of  C. rotundus  on oxidative protein damages by decreasing oxidative stress ( Ardestani & Yazdanparast, 2007 ). Anti-oxidant activity and metal chelating properties of  C. rotundus  are shown by Fluorescence Recovery After Photobleaching (FRAP) and Trolox Equivalent Antioxidant Capacity (TEAC) assays ( Ardestani & Yazdanparast, 2007 ). This herbal extract with anti-inflammatory properties is safe in animals ( Dang, Parekar, Kamat, Scindia, & Rege, 2010 ;  Seo et al., 2001 ).\nIt seems that the antioxidant properties of  C. rotundus  are due to its phenolic content ( Ardestani & Yazdanparast, 2007 ), which should be studied further. In conclusion, intrahippocampal injection of Aβ induced significant learning deficits in Morris water-maze tasks, and  C. rotundus  treatment improved Aβ-induced deficiencies. Therefore, it is likely that  C. rotundus  may be useful to treat patients with impaired memory function.","source_license":"CC-BY-4.0","license_restricted":false}