{"paper_id":"d3c2c2ba-fee1-4f39-b00f-980e3d9241cc","body_text":"Abstract\nObjective: To determine [1] expression levels of both DNA methyltransferases (DNMTs) and methyl-CpG-binding domain proteins (MBDs) in human endometrium throughout the menstrual cycle and in eutopic and ectopic endometrium of patients with endometriosis and [2] hormone responsiveness of DNMT and MBD expression in explant cultures of proliferative phase endometrium. Design: In vitro study. Setting: Academic medical center. Patient(s): Premenopausal women with and without endometriosis. Intervention(s): Explant cultures of proliferative phase endometrium were treated with vehicle, 17 beta-E(2), or a combination of E(2) and P (E(2) + P) for 24 hours. Main Outcome Measure(s): Expression levels of DNMT1, DNMT2, and DNMT3B and MBD1, MBD2, and MeCP2 with use of real-time quantitative polymerase chain reaction. Result(s): Expression levels of DNMT1 and MBD2 were significantly higher in secretory-phase endometrium compared with proliferative endometrium and menstrual endometrium. In explant cultures, treatment with E(2) + P resulted in significant up-regulation of DNMT1 and MBD2. Expression levels of several DNMTs and MBDs were significantly lower in endometriotic lesions compared with eutopic endometrium of women with endometriosis and disease-free controls. Conclusion(s): These findings suggest a role for DNMTs and MBDs in the growth and differentiation of the human endometrium and support the notion that endometriosis may be an epigenetic disease. (Fertil Steril (R) 2011;95:1421-7.)\n| Original language | English |\n|---|---|\n| Pages (from-to) | 1421-1427 |\n| Number of pages | 7 |\n| Journal | Fertility and Sterility |\n| Volume | 95 |\n| Issue number | 4 |\n| DOIs | |\n| Publication status | Published - Mar 2011 |\nKeywords\n- Endometriosis\n- endometrium\n- DNA methylation\n- epigenetics\n- DNMT\n- MBD\nFingerprint\nDive into the research topics of 'Deoxyribonucleic acid methyltransferases and methyl-CpG-binding domain proteins in human endometrium and endometriosis'. Together they form a unique fingerprint.Cite this\n- APA\n- Author\n- BIBTEX\n- Harvard\n- Standard\n- RIS\n- Vancouver","source_license":"public-domain-us","license_restricted":false}