{"paper_id":"d22d873c-a03d-4799-9125-77e027b05c9d","body_text":"Critical Reviews™ in Eukaryotic Gene Expression\nPublished 8 issues per year\nISSN Print: 1045-4403\nISSN Online: 2162-6502\nResearch Article\nPrototype Salivary Assay for Quantification of Two Biomarkers for In Vitro Diagnosis of Endometriosis\nABSTRACT\nEndometriosis, a very common disease in women, is characterized by endometrial structures outside the uterine cavity. The lack of a reliable noninvasive diagnostic test and the often nonspecific symptoms of this pathology are responsible for the delay in definitive diagnosis of this disease. Recently, through a proteomics approach, our research group has identified two potential diagnostic markers for endometriosis in serum (Zn-alpha2-glycoprotein and complement C3 precursor). In this article, we describe the experimental conditions of a simple ELISA for rapid quantification of these two biomarkers in the saliva of patients with endometriosis. Finally, preliminary experiments on a small cohort of patients and controls have confirmed the potential diagnostic value of this assay.\nFigures\n-\nSignorile PG, Campioni M, Vincenzi B, D'Avino A, Baldi A. Rectovaginal septum endometriosis: An immunohistochemical analysis of 62 cases. In Vivo. 2009;23:459-64.\n-\nGiudice LC, Khao LC. Endometriosis. Lancet. 2004;364:1789-99.\n-\nSignorile PG, Cassano M, Viceconte R, Spyrou M, Marcattilj V, Baldi A. Endometriosis: A retrospective analysis on diagnostic data in a cohort of 4401 patients. In Vivo. 2022;36:430-38.\n-\nSignorile PG, Cassano M, Viceconte R, Spyrou M, Marcattilj V, Baldi A. Endometriosis: A retrospective analysis of clinical data in a cohort of 4,083 patients, with focus on symptoms. In Vivo. 2022;36:874-83.\n-\nJohnson NP, Hummelshoj L, Adamson GD, Keckstein J, Taylor HS, Abrao MS, Bush D, Kiesel L, Tamimi R, Sharpe-Timms KL, Rombauts L, Giudice LC. World endometriosis society; Sao Paulo consortium. 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