{"paper_id":"cc7c6f62-b314-4df1-9036-fdf57b0c1598","body_text":"Abstract\nBackground: Extensive endometriosis causes a mechanical disturbance in the pelvis leading to obstructive-type infertility. However, minimal or mild endometriosis is suspected to cause infertility, possibly through a humoral agent. Previous studies reported the presence of a factor in the serum of patients with endometriosis which reduced fertilization and early embryo formation in a rat IVF model.\nMethods: In the present article, we report a comparison of oocytes exposed to endometrioma fluid and oocytes not exposed (controls) in the context of a human IVF setting. We have been in the practice of aspirating oocytes into prewarmed 60-ml syringes containing culture medium. We have shown previously that this technique reduces the length of oocyte retrieval without compromising success. In 14 women undergoing oocyte retrieval, we inadvertently entered an endometrioma. This resulted in retrieved oocytes that were either exposed or not exposed to endometrioma fluid.\nResults: In contrast to previous reports, we found no difference in fertilization or early embryo development between the two groups. The fertilization rate for oocytes exposed to an endometrioma was 60%, versus 56% for controls. The good-quality embryo formation rate for oocytes exposed to an endometrioma was 45%, versus 46% for controls.\nSimilar content being viewed by others\nREFERENCES\nKhamsi F, Lacanna I, Endman M: Recent advances in assisted reproductive technologies. Endocrine 1998;9:15-25\nKhamsi F, Roberge S, Yavas Y: In vitro fertilization and intracytoplasmic sperm injection into the oocyte at Toronto Fertility Sterility Institute (14-year evolution of an independent health facility). Ann Roy Coll Phys Surg Can 1999;32:82-86\nMiller KA, Pittaway DE, Deaton JL: The effect of serum from infertile women with endometriosis on fertilization and early embryonic development in a murine in vitro fertilization model. Fertil Steril 1995;64:623-626\nSAS: Statistical Analysis System. SAS/STAT User's Guide. Release 6.03 ed. Cary, NC, Statistics Institute, 1988.\nSchmidt CL: Endometriosis: A reappraisal of pathogenesis and treatment. In Modern Trends in Infertility and Conception Control, EE Wallach, RD Kempers (eds). Chicago, Year Book Medical, 1988, pp 325-341\nWebster BW, Wentz AC, Maxson WS: Endometriosis. In Reproductive Surgery, AH DeCherney, ML Polan (eds). Chicago, Year Book Medical, 1999, pp 221-242\nAuthor information\nAuthors and Affiliations\nRights and permissions\nAbout this article\nCite this article\nKhamsi, F., Yavas, Y., Lacanna, I.C. et al. GYNECOLOGY: Exposure of Human Oocytes to Endometrioma Fluid Does Not Alter Fertilization or Early Embryo Development. J Assist Reprod Genet 18, 106–109 (2001). https://doi.org/10.1023/A:1026586709554\nIssue date:\nDOI: https://doi.org/10.1023/A:1026586709554","source_license":"CC0","license_restricted":false}