{"paper_id":"cb2283f7-e327-46c6-b8db-33ff3b68e795","body_text":"Endometriosis manifests as ectopic endometrial cells outside the uterus. It is an intractable disease that causes infertility, dysmenorrhea and pelvic pain. Endometriosis occurs in 10% of women of childbearing age. Notably, the incidence of endometriosis has been rising in recent years ( 1 ). The pathogenesis of endometriosis remains to be elucidated.\nPublished reports indicate that endometriosis is a polygenic/multifactorial disease caused by interactions between multiple genes and the environment ( 2 , 3 ). In particular, a correlation has been identified between endometriosis and exposure to environmental toxins such as dioxin ( 4 ); dioxin and dioxin-like compounds have been implicated in the development of endometriosis ( 5 , 6 ).\nThe phase II conjugation enzymes usually function to inactivate environmental toxins. Among these, glutathione S-transferase (GST) may be critical for the detoxification of dioxins. Human GSTs are classified into two distinct categories: Soluble or cytosolic and membrane-bound microsomal. The soluble or cytosolic GSTs are subdivided into seven families named α, µ, ω, π, σ, θ and ζ ( 7 ). Genes in several of these families are polymorphic, including:  GSTA2  in the α family,  GSTM1  and  GSTM3  in the µ family,  GSTP1  in the π family,  GSTO ,  GSTT1 , and  GSTT2  in the θ family, and  GSTZ1  in the ζ family. Heritable allelic differences in  GSTM1 ,  GSTM3 ,  GSTT1  and  GSTP1  may have marked relevance for individual susceptibility to disease.\nGSTM1  and  GSTT1  are two candidate genes that may play an important role in the development of endometriosis.  GSTM1  and  GSTT1  are located on chromosomes 1p13.3 and 22q11.23, respectively. They are critical in the detoxification of the products of oxidative stress produced during the repair of the ovarian epithelium.  GSTM1  and  GSTT1  null alleles have reduced enzyme activity, a state that may contribute to inefficient detoxification of intermediates produced during stress. This may increase damage to various host genes and contribute to the pathogenesis of endometriosis ( 8 , 9 ).\nA meta-analysis summarizing the literature up to the year 2005 suggested that the  GSTT1  null genotype, but not the  GSTM1  null genotype, was associated with an increased risk for endometriosis ( 7 ). In the years since 2005, additional reports investigating this topic have been published. The objective of the present study was to update the existing meta-analysis and reevaluate the possible associations between  GSTM1 ,  GSTT1  and combined  GSTM1 / GSTT1  (null genotype vs. wild-type) gene polymorphisms and susceptibility to endometriosis.\n\nFor this systematic review and meta-analysis, PubMed (from January 1996 to January 2014), Embase (from January 1996 to January 2014), Chinese BioMedical Literature database (from January 1996 to January 2014) and Google Scholar (from January 1996 to January 2014) were searched. The following keywords were used: ‘endometriosis’, ‘polymorphisms’, ‘glutathione S-transferases’, ‘ GSTM1 ’ and ‘ GSTT1 ’ or their combinations.\nReference lists from articles identified by the electronic search were searched by hand. This process was performed iteratively until no additional articles could be identified.\nArticles published in English or Chinese were included if they reported quantitative outcomes from case-control genetic association studies on  GSTM1 ,  GSTT1  or combined  GSTM1 / GSTT1  (null genotype vs. wild-type) gene polymorphisms and endometriosis versus non-endometriosis or healthy controls.\nStudies were excluded if they were case reports, case-only studies, letters, reviews or meta-analyses; included subjects who were related; included cases of adenomyosis, which has unknown etiology ( 10 ); reported insufficient data; or were duplicate studies.\nTwo reviewers (XYX and ZSJ) independently examined titles and abstracts to select eligible studies. Records were removed that were ongoing or unpublished studies, or were published as abstracts or conference proceedings. Where data sets were overlapping or duplicated, only the most recent information was included. The full text of potentially relevant studies was retrieved. Two reviewers (XYX and HJG) independently examined the full text records to determine which studies met the inclusion criteria. Disagreement about the selection of studies was resolved by discussion and consensus.\nTwo reviewers (XYX and ZSJ) independently extracted data from eligible studies including the first author's last name, publication year, study location, ethnicity, matching variability, diagnostic criteria, stages of disease, source of controls, numbers of cases and controls, and numbers and/or percentages of null genotypes. Disagreement about data extraction was resolved by discussion and consensus.\nTwo reviewers (YYL and HJG) independently assessed quality of evidence in the included studies using the 9-star Newcastle-Ottawa Scale, which considers selection, comparability and outcome evaluation criteria.\nHeterogeneity was assessed using the χ2 test and I2 test. The I2 statistic was interpreted as follows: I2=0–40%, heterogeneity may not be important; I2= 30–60%, heterogeneity may be moderate; I2=50–90%, heterogeneity may be substantial; and I2=75–100%, considerable heterogeneity ( 11 ). If heterogeneity was present, meta-regression was used to find the source.\nA funnel plot of effect estimates against their standard errors (SEs) was created to assess possible reporting bias between studies. Funnel plot asymmetry was assessed using Egger's linear regression test and Begg's rank correlation test; P<0.05 suggested publication bias.\nTwo reviewers (XYX and HJG) independently combined data from trials using a fixed-effect model (DerSimonian and Laird method) when there was no significant heterogeneity in populations (I2<50%) and a random-effect model (Mantel-Haenszel method) when there was considerable heterogeneity. Variables were synthesized using odds ratios (ORs). A P-value of 0.05 was used as the cut-off value to determine statistical significance, and data are presented as the estimated OR with 95% confidence intervals (CIs). All statistical analyses were performed using STATA software, version 12.0 (StataCorp, College Station, TX, USA). Inconsistencies in data analysis were resolved through consensus and discussion with a third reviewer (ZSJ).\nSensitivity analyses were performed to explore the impact of excluding outlying results. Subgroup analyses were performed by stratifying patients according to ethnicity (Caucasian, Asian or mixed), characteristics of controls (hospital patients or healthy individuals), and quality of evidence (high-quality or low-quality).\n\nThe searches identified 120 articles. Titles and abstracts were screened, and 36 studies were identified as potentially eligible for inclusion. The full text articles for these studies were retrieved. Following analysis of the full text articles, four studies were excluded and 32 studies were found to be eligible for inclusion according to the criteria used for considering studies in this review ( Fig. 1 ).\nThe characteristics of the included studies are shown in  Table I . There were 32 case-control genetic association studies involving 3,990 cases of endometriosis and 4,625 controls. One publication addressed two groups of subjects with different ethnicities and was considered as two case-control genetic association studies ( 12 ); thus, the total number of studies was considered to be 33. Studies included data relevant to the  GSTM1  genotype,  GSTT1  genotype or the combined  GSTM1 / GSTT1  genotype. Of the 32 eligible studies, 20 were conducted in Asia ( 12 – 31 ), eight in Europe ( 32 – 39 ), two in North America ( 40 , 41 ), and two in South America ( 42 , 43 ). The evidence reported in 23 studies was identified as high-quality, and that in 10 studies was identified as low-quality.\nOf the 36 studies that were relevant to the  GSTM1 / GSTT1  genotype and endometriosis, four were excluded. Of these, three were duplicates ( 13 , 14 , 32 ), and one included subjects who were related ( 44 ).\nData reporting on the  GSTM1  gene polymorphism are described in 33 case-control studies (3,990 cases of endometriosis and 4,625 controls). The meta-analysis demonstrated that there was a significant association between the  GSTM1  null genotype and an increased risk for endometriosis (OR=1.56; 95% CI: 1.25–1.95; P<0.0001;  Fig. 2A ).\nSubgroup analyses stratified by ethnicity (Caucasian: OR=1.599; 95% CI: 1.205–2.122; P=0.001; Asian: OR=1.772; 95% CI: 1.242–2.528, P=0.002), source of controls (hospital patients: OR=1.561; 95% CI: 1.151–2.117; P=0.004; healthy individuals: OR=1.569; 95% CI: 1.131–2.176; P=0.007), and quality of evidence (high-quality: OR=1.563; 95% CI: 1.253–1.949; P<0.0001) confirmed this finding.\nSubgroup analysis stratified for mixed ethnicity (two case control studies involving 111 cases of endometriosis and 78 controls) demonstrated a significant association between the  GSTM1  null genotype and a decreased risk for endometriosis (OR=0.404; 95% CI: 0.219–0.745; P=0.004;  Table II ). Compared with individual Caucasian and Asian populations, the difference was statistically significant (P<0.001; data shown in  Table III ).\nData reporting on the  GSTT1  gene polymorphism are described in 18 case-control studies (2,371 cases of endometriosis and 2,490 controls). The meta-analysis demonstrated a significant association between the  GSTT1  null genotype and an increased risk for endometriosis (OR=1.31; 95% CI: 1.02–1.68; P=0.037;  Fig. 2B ).\nSubgroup analysis stratified by ethnicity demonstrated a significant association between the  GSTT1  null genotype and an increased risk for endometriosis among Asians (OR=1.573; 95% CI: 1.186–2.085; P=0.002), but not among Caucasians (OR=1.124; 95% CI: 0.745–1.697; P=0.577).\nSubgroup analyses stratified by the source of controls found no significant association between the  GSTT1  null genotype and an increased risk for endometriosis among hospital-based studies (OR=1.284; 95% CI: 0.963–1.712; P=0.089) or among healthy individuals (OR=1.315; 95% CI: 0.767–2.254; P=0.320).\nSubgroup analyses stratified by quality of evidence demonstrated a significant association between the  GSTT1  null genotype and an increased risk for endometriosis among studies considered high-quality evidence (OR=1.376; 95% CI: 1.020–1.858; P=0.037), but not among studies considered low-quality evidence (OR=1.121, 95% CI: 0.646–1.944; P=0.684;  Table II ).\nData reporting on the combined  GSTM1 / GSTT1  gene polymorphism are described in eight case-control studies (1,083 cases of endometriosis and 1,222 controls). The meta-analysis demonstrated a significant association between the combined  GSTM1 / GSTT1  null genotype and an increased risk for endometriosis (OR=1.68, 95% CI: 1.29–2.17; P<0.0001;  Fig. 2C ).\nThis association was unchanged by subgroup analyses stratified by source of controls (hospital-based studies: OR=1.797; 95% CI: 1.081–2.989; P=0.024; healthy individuals: OR=1.657; 95% CI: 1.085–2.532; P=0.001) or quality of evidence (high-quality evidence: OR=1.753; 95% CI: 1.265–2.430; P=0.001; low-quality evidence: OR=1.542; 95% CI: 1.009–2.356, P=0.045;  Table II ).\nSubgroup analysis stratified by ethnicity demonstrated a significant association between the combined  GSTM1 / GSTT1  null genotype and an increased risk for endometriosis among Asian populations (OR=1.898; 95% CI: 1.404–2.565; P<0.001), but not among Caucasian populations (OR=1.185; 95% CI: 0.717–1.961; P=0.508).\nVisual inspection of a Funnel plot, Egger's test and Begg's rank correlation test revealed no significant publication bias for the  GSTM1 ,  GSTT1  and combined  GSTM1 / GSTT1  studies ( Fig. 3 ;  Table IV ).\nThere was evidence of significant heterogeneity (I2>50%) between studies of  GSTM1  and  GSTT1 , and those used in subgroup analyses, although not among studies of  GSTM1 / GSTT1  combined ( Table IV ). Therefore, the random-effect model was used in all analyses with the exception of the analysis of combined  GSTM1 / GSTT1  gene polymorphisms. For the  GSTM1  and  GSTT1  gene polymorphisms, a meta-regression was conducted in which publication year, ethnicity, source of controls, sample size, and quality of evidence were covariates. All the covariates were entered into the meta-regression model simultaneously, and the covariates that had the highest P-values were omitted one at a time in order to identify any sources of heterogeneity among them. However, the meta-regression analysis did not identify any of these covariates as a significant source of heterogeneity ( Figs. 4  and  5 ).\nTo explore the effects of individual studies on the pooled OR estimates, a sensitivity analysis was performed, with the omission of one study at a time. The OR estimates for the  GSTM1  polymorphism were not notably altered ( Fig. 6A ). The OR estimates for the  GSTT1  and combined  GSTM1 / GSTT1  polymorphisms were altered when studies were excluded ( Fig. 6B and C ).\n\nIn the present study, a meta-analysis of data from 33 studies was conducted to examine the associations between the  GSTM1 ,  GSTT1  and combined  GSTM1 / GSTT1  null genotypes and susceptibility to endometriosis. The risk for endometriosis was significantly increased in the presence of the  GSTM1 ,  GSTT1  and combined  GSTM1 / GSTT1  null genotypes compared with the wild-type. Subgroup analyses stratified by ethnicity, source of controls and quality of evidence confirmed this finding among several subgroups, but particularly among studies considered high-quality evidence. Notably, among patients of mixed ethnicity, the  GSTM1  null genotype was significantly associated with a decreased risk for endometriosis compared with the wild-type.\nA similar meta-analysis of 23 studies performed in 2005 demonstrated an increased risk for endometriosis in women with the  GSTT1  null genotype ( 8 ). However, the authors requested that their findings be interpreted with caution as asymmetry in the funnel plot was evident, which was likely due to publication bias ( 8 ). This previous study did not include subgroup analyses or an evaluation of the combined  GSTM1 / GSTT1  null genotype-endometriosis association.\nPrevious meta-analyses have found that the  GSTM1 / GSTT1  gene polymorphism is associated with cervical cancer ( 45 ), breast cancer ( 46 ), bladder cancer ( 47 ), gastric cancer ( 48 , 49 ) and acute leukemia ( 50 ). In accordance with the observations of the present study, several studies have shown that the  GSTM1  (OR=32.6, 95% CI: 15.07–70.32, P<0.0001) ( 18 ) and  GSTT1  (OR>3; P<0.0001) null genotypes ( 12 , 16 ) are associated with an increased risk for endometriosis. However, other reports suggest the  GSTM1  (OR=0.21, 95% CI: 0.09–0.52, P<0.0001; OR=0.35, 95% CI: 0.15–0.83, P<0.0001) ( 40 , 43 ),  GSTT1  (OR≥5; P<0.0001) ( 16 ) and combined  GSTM1 / GSTT1  (OR=0.38; P<0.001) ( 17 ) null genotypes are associated with a decreased risk for endometriosis. These divergent results may be explained by differences in  GSTM1 / GSTT1  null genotype frequencies and study locations. The frequency of the  GSTM1 / GSTT1  null genotype may vary from 10 to 65% depending on the region and population studied ( 51 ). Different study locations may introduce confounding variables associated with variations in lifestyles and exposures to toxic substances of the study populations.\nThe results of the present study must be interpreted with caution due to the presence of substantial heterogeneity. Among analyses of the studies of  GSTM1  and  GSTT1 , the cause of heterogeneity remains unclear, despite meta-regression analyses being conducted. Among the analyses of combined  GSTM1 / GSTT1  studies, subgroup and sensitivity analyses suggested that studies that included patients with advanced stage endometriosis caused most of the variability. Publication bias was unlikely to have influenced the findings.\nIn addition to the heterogeneity, there were several limitations to this study. Firstly, the composition of the endometriosis patient and control populations varied between studies. For instance, some studies included only patients with advanced endometriosis ( 17 – 20 , 22 , 27 , 35 ), while control populations consisted of a mixture of infertile ( 29 ), postmenopausal ( 43 ) and premenopausal ( 18 , 35 ) women, and newborn babies that had not been exposed to the environment ( 17 ). Furthermore, patients and controls were not always accurately matched by age or environmental exposures. Secondly, gene-gene or gene-environment interactions may jointly increase the risk for endometriosis; therefore, different lifestyle and environmental factors may contribute to differential genotypic frequencies in cases and controls. Attempts were made to mitigate inaccuracies associated with this limitation through a subgroup analysis stratified according to ethnicity. Thirdly, this study was based on published articles. As a positive result is more likely to be published, publication bias is an inherent limitation of all meta-analyses irrespective of the outcomes of the Egger's linear regression test and Begg's rank correlation test.\nIn conclusion, the present meta-analysis shows the  GSTM1 ,  GSTT1  and combined  GSTM1 / GSTT1  null genotypes are likely associated with increased susceptibility to endometriosis. These data are in contrast to those reported previously. Therefore, further studies reporting higher quality evidence are necessary to verify these conclusions.","source_license":"CC0","license_restricted":false}