{"paper_id":"b4972a2d-7daa-4e3e-8b86-e2bb5cfdfebf","body_text":"Abstract\nAutophagy has emerged as an important process of cell metabolism. With continuous in-depth research on autophagy, TFEB has been a key transcription factor regulating autophagy levels in recent years. Studies have established that TFEB regulates autophagy and apoptosis in various diseases. However, the relationship between TFEB and the pathogenesis of endometriosis remains unclear. This study aimed to investigate the effect of TFEB on the mechanism of endometriosis progression. The results showed that TFEB and autophagy-related protein LC3 are highly expressed in ectopic endometrium of patients with endometriosis, overexpression of TFEB in cultured human endometrial stromal cells (HESCs) by lentivirus not only promoted autophagy but also inhibited apoptosis. In addition, the migration and invasion ability of HESCs were enhanced by TFEB overexpression. Furthermore, inhibiting autophagy with specific inhibitors can attenuate migration and invasion of HESCs induced by TFEB. The rat models of endometriosis show that TFEB knockdown can suppress lesion growth in vivo. Our results suggest that autophagy may be involved in the progression mechanism of endometriosis, and the mechanism of autophagy disorder in endometriosis is probably related to TFEB. TFEB may be a key molecule in promoting endometriosis.\nSimilar content being viewed by others\nData availability\nThe data that support the findings of this study are available on resquest from the corresponding author.\nAbbreviations\n- CQ:\n-\nChloroquine\n- HESCs:\n-\nHuman endometrial stromal cells\n- mTORC1:\n-\nMammalian target of rapamycin complex 1\n- TFEB:\n-\nTranscription factor EB\nReferences\nLiu Y, Wang J, Zhang X (2022) An update on the multifaceted role of NF-kappaB in endometriosis. 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Front Cell Dev Biol 9:787278 Epub 2022/02/01. https://doi.org/10.3389/fcell.2021.787278\nAcknowledgements\nThe authors thank the staff of the Second Affiliated Hospital of Wenzhou Medical.\nFunding\nThis study was financially supported by the Natural Science Foundation of Zhejiang Province [grant No. LY20H040005]. The Public Welfare Science and Technology Project of Wenzhou City [grant No. Y20210021]. The Medical Science and Technology Project of Zhejiang Province [grant No.2023KY145]. The Natural Science Foundation of Zhejiang Province [grant No. LY23H040003]. The Medical Science and Technology Project of Zhejiang Province [grant No. 2022KY212]. The Zhejiang Province Traditional Chinese Medicine Science and Technology Project [grantNo.2023ZL510]. The Public Welfare Science and Technology Project of Wenzhou City [grant No. Y2020087].\nAuthor information\nAuthors and Affiliations\nContributions\nZhao Yu and Qiong Zhang designed the experiments and revised manuscript; Chen Qiuyu and Sennan Zhu conducted experiments and wrote the main manuscript; Yu Meng Qi and Zhou Yi analysed data; Sun Jindan and Du Wenzhuo prepared Figs. 1 and 2; Chen Ziqi, Tao Jiayu and Feng Xiao prepared 3, 4, 5, 6 and 7.\nCorresponding authors\nEthics declarations\nStatements and declarations\nThe research results reported in this article are all original research results of myself or all members of our research group.\nCompeting interests\nThe authors declare no competing interests.\nEthical approval\nThis study was approved by the Ethics Committee of Wenzhou Medical University Affiliated Second Hospital and Yuying Children’s Hospital. (Ethical code LCKY209-132)\nAdditional information\nPublisher’s Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nSpringer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.\nAbout this article\nCite this article\nChen, Q., Zhou, Y., Yu, M. et al. Transcription factor EB-mediated autophagy affects cell migration and inhibits apoptosis to promote endometriosis. Apoptosis 29, 757–767 (2024). https://doi.org/10.1007/s10495-024-01939-4\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s10495-024-01939-4","source_license":"CC0","license_restricted":false}