{"paper_id":"aa008083-fecd-492e-a7e7-056e089a8f10","body_text":"Progesterone resistance in endometriosis is modulated by the altered expression of microRNA-29c and FKBP4\nPublic DepositedAdd to collection\nYou do not have access to any existing collections. You may create a new collection.\nDownloadable Content\nDownload PDFCitation\nMLA\nJoshi, Niraj R, et al. Progesterone Resistance In Endometriosis Is Modulated by the Altered Expression of Microrna-29c and Fkbp4. 2016. https://doi.org/10.17615/h6k0-pt92APA\nJoshi, N., Miyadahira, E., Afshar, Y., Jeong, J., Young, S., Lessey, B., Serafini, P., & Fazleabas, A. (2016). Progesterone resistance in endometriosis is modulated by the altered expression of microRNA-29c and FKBP4. https://doi.org/10.17615/h6k0-pt92Chicago\nJoshi, Niraj R., Eduardo H Miyadahira, Yalda Afshar, Jae Wook Jeong, Steven L Young, Bruce A Lessey, Paulo C Serafini et al. 2016. Progesterone Resistance In Endometriosis Is Modulated by the Altered Expression of Microrna-29c and Fkbp4. https://doi.org/10.17615/h6k0-pt92- Creator\n-\nJoshi, Niraj R.\n- Other Affiliation: Department of Obstetrics; Gynecology and Reproductive Biology; Michigan State University\n-\nMiyadahira, Eduardo H.\n- Other Affiliation: Huntigton Medicina Reprodutiva\n-\nAfshar, Yalda\n- Other Affiliation: Department of Obstetrics and Gynecology; University of California\n-\nJeong, Jae-Wook\n- Other Affiliation: Department of Obstetrics; Gynecology and Reproductive Biology; Michigan State University\n-\nYoung, Steven L.\n- School of Medicine, Department of Obstetrics and Gynecology\n-\nLessey, Bruce A.\n- Other Affiliation: Greenville Hospital System; University of South Carolina; School of Medicine\n-\nSerafini, Paulo C.\n- Other Affiliation: Discipline of Gynecology; Department of Obstetrics and Gynecology; Hospital das Clinicas; Faculdade de Medicina; Universidade de São Paulo\n-\nFazleabas, Asgerally T.\n- Other Affiliation: Department of Obstetrics; Gynecology and Reproductive Biology; Michigan State University\n-\nJoshi, Niraj R.\n- Abstract\n- Context: Endometriosis results in aberrant gene expression in the eutopic endometrium (EuE) and subsequent progesterone resistance. MicroRNA (miR) microarray data in a baboon model of endometriosis showed an increased expression of miR-29c. Objectives: To explore the role of miR-29c in progesterone resistance in a subset of women with endometriosis. Design: MiR-29c expression was analyzed in the endometrium of baboons and women with or without endometriosis. The role in progesterone resistance and decidualization was analyzed by transfecting human uterine fibroblast cells with miR-29c. Patients: Subjects diagnosed with deep infiltrative endometriosis (DIE) by transvaginal ultrasound with bowel preparation underwent surgical excision of endometriosis. Eutopic secretory endometrium was collected pre- and postoperatively. Women with normal EuE and without DIE served as controls. Results: Quantitative reverse transcription polymerase chain reaction demonstrated that miR-29c expression increased, while the transcript levels of its target, FK506-binding protein 4 (FKBP4), decreased in the EuE of baboons following the induction of endometriosis. FKBP4 messenger RNA and decidual markers were statistically significantly decreased in decidualized human uterine fibroblast cells transfected with a miR-29c mimic compared with controls. Human data corroborated our baboon data and demonstrated higher expression of miR-29c in endometriosis EuE compared with normal EuE. MiR-29c was significantly decreased in endometriosis EuE postoperatively compared with preoperative tissues, and FKBP4 showed an inverse trend following radical laparoscopic resection surgery. Conclusions: We demonstrate that miR-29c expression is increased in EuE of baboons and women with endometriosis, which might contribute to a compromised progesterone response by diminishing the levels of FKBP4. Resection of DIE is likely to reverse the progesterone resistance associated with endometriosis in women.\n- Date of publication\n- 2016\n- Keyword\n- DOI\n- Identifier\n- PMID: 27778641\n- PMCID: PMC5413101\n- Onescience id: 62db1b51f1c087a708c7ec5791b8b271793d454b\n- Publisher DOI: https://doi.org/10.1210/jc.2016-2076\n- Resource type\n- Article\n- Rights statement\n- In Copyright\n- Journal title\n- Journal of Clinical Endocrinology & Metabolism\n- Page start\n- jc.2016-2076\n- Language\n- English\n- ISSN\n- 1945-7197\n- 0021-972X\nRelations\n- Parents:\nThis work has no parents.\nItems\n| Thumbnail | Title | Date Uploaded | Visibility | Actions |\n|---|---|---|---|---|\n| jc.2016-2076.pdf | 2016 | Public | Download |","source_license":"CC0","license_restricted":false}