{"paper_id":"a2ae50c0-b7f4-4f17-bb59-c8a07005dfdc","body_text":"Chronic pelvic pain (CPP) is a frequent complaint in the gynecological practice, and it causes suffering, compromises the quality of life of the woman, and results in high costs to health systems.\n 1 \nThe prevalence of CPP is not well established, and it may vary from one country to another. However, it is estimated that 3.8% of women aged between 15 and 73 years, and between 14% to 24% of women of reproductive age present CPP.\n 2 \nIn Brazil, the prevalence is not well known; international studies have demonstrated a high prevalence of persistent pain in Brazilian women, ∼ 36% in Rio de Janeiro, and 13% of women working in São Paulo.\n 3 \nIn the city of Ribeirão Preto, the detected prevalence was of 11.5%, and 15.1% of these women are of reproductive age.\n 4\nEndometriosis is among the gynecological causes related to CPP, and its main clinical problem is painful syndrome, manifesting as dysmenorrhea, pelvic pain, abdominal pain, dyspareunia, and painful defecation.\n 5 \nIn women with CPP subjected to laparoscopy, the presence of endometriosis is higher than 30%.\n 2 \nThe mechanisms involved are not clear yet, and the nature of the pain associated with endometriosis has been poorly characterized. Evidence suggests that the pain may be caused by peritoneal inflammation, formation of adherences, and significant nervous injury, specific to endometriosis injuries, which are possibly correlated with a deep infiltration of the endometrial tissue.\n 6\nThe scientific evidence of the relationship of overweight and obesity in women with CPP has not yet been clarified. The association between BMI and endometriosis has been studied; Viganò et al\n 7 \nconcluded that women with endometriosis tend to be thinner than women without the disease, and the severity of the disease seems to be associated with the BMI. Yi et al\n 8 \nshowed that women with advanced diseases had lower BMIs than those with minimal or mild diseases.\nEndometriosis is closely associated with alterations of the immune and endocrine systems,\n 9 \nwhich are systems that are also associated with the control of obesity. To identify the etiological or mediating pathways associated with adiposity and endometriosis, one study\n 10 \nhas suggested that the adipose tissue has immune properties. It is possible that these immune properties of the adipose tissue are involved or affected by the development of endometriosis, which can arise from an alteration in immune functioning.\n 9 \nThe inhibition or promotion of immune cells may be linked to inflammation and to stimulation of angiogenesis.\n 12\nOverweight has a high prevalence among females, and it is even higher during perimenopause: it may affect 60% of the women in this stage of life, probably due to metabolic alterations inherent to this period. It is also associated with bad eating habits and genetic predisposition.\n 13 \nThe BMI and the percentage of body fat (%BF) may be involved in the relationship between overweight and obesity, which can influence the report of painful perception in women with CPP. Han et al\n 14 \nreported that an elevated waist-to-hip ratio (WHR)\n 15 \nindicates a pattern of central obesity, and it was significantly associated with chronic low-back pain in women, but not in men. Planning an effective treatment might help in the early identification of problems related to CPP secondary to endometriosis, and reducing and relieving the distress suffered by these women is of great importance. Therefore, the present study aimed to evaluate whether there is a difference in average body composition (%BF) through an evalution of the anthropometric markers of women diagnosed with CPP secondary to endometriosis and those diagnosed with CPP secondary to other causes. Moreover, we aimed to correlate the average body composition (%BF) with the intensity of the clinical pain in these women.\n\nThe description of the present study was developed according to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE)\n 16 \nguidelines (\n http://www.strobe-statement.org \n).\nThe present observational case-control study was approved by the Ethics Committee of the teaching hospital at Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (HCFMRP-USP), as well as its informed consent form (ICF). Women diagnosed with CPP at the outpatient clinic of pelvic pain were invited to participate in the study before starting any treatment.\nThe adopted inclusion criteria were women aged between 18 and 49 years, with CPP secondary to endometriosis or to other possible causes for at least 6 months, intense enough to interfere in routine activities, and demanding clinical or surgical treatment. All patients were submitted to laparoscopy and histology for the diagnosis of endometriosis. Women at menopause, those who were smokers, pregnant, and breastfeeding for the previous six months were excluded from the study, as well as those who refused to participate. Informed consent was obtained before the intervention and after the patients received explanations.\nThe patients who agreed to participate in the study were scheduled for an evaluation and advised to fast for 12 hours and to not change their eating behavior,\n 17 \nto not have products with caffeine: coffee, tea, and chocolate,\n 18 \nalcohol,\n 19 \nor medication\n 20 \nin the 24 hours preceding the interview, in which questionnaires were filled out and data were collected. Age, anthropometric data, (body mass, height), the perimeters of the arm, waist, abdomen, and hip, as well as the %BF were recorded.\nTo measure body mass and height, the women had to be barefoot, with no excess of clothes or accessories, with an erect body, the feet together, and the arms extended along the body, looking at a fixed point in the horizon.\n 21 \nMeasurements were made using a Filizola (São Paulo, SP, Brazil) digital scale (0.1-kg scale), with a capacity for 150 kg with a coupled stadiometer. With the combination of body mass and height, we calculared the BMI (BMI = body mass/height\n 2 \n).\n 22\nTo verify the perimeters of the arm, waist, abdomen, and hip, a Sanny (São Bernardo do Campo, SP, Brazil) non-elastic tape with 1 mm of precision was used. According to\n 23 \nthe perimeter measurements, mainly the waist and hip perimeters are widely used, since they are fundamental to body composition.\nThe regional distribution of the %BF arouses concern by virtue of the association among health complications resulting from metabolic and cardiovascular dysfunctions and a larger accumulation of fat in the central region of the body.\nThe %BF was evaluated using the Model 310e. Bioimpedance Body Composition Monitor (Biodynamics Corporation, Shorelaine, WA, US). It sends a subthreshold electric current (800 µA, 50 kHz – single frequency), with gel electrodes (Hearbeat, Conmed corp., NY, USA) for bioimpedance.\n 24 \nIt is a monitor that dysplays in a fast and precise way the amount of fat mass, lean mass, total body water, basal energy metabolism, and ideal weight. Its principle is based on electrical bioimpedamce, showing correlation levels, comparing to more accurate methods existing today.\n 25 \nTo compare the %BF, we used values of ideal %BF according to Lohman,\n 26 \nclassifying percentage from low (10% to 15%) to very high (> 30%).\nTo evaluate the intensity of the clinical pain, the visual analog scale (VAS) was used.\n 27 \nIt consists of a 10-cm uninterrupted line in which the patient is instructed to check the score corresponding to the referred pain, keeping in mind that the beginning of the scale (0) corresponds to (no pain), and the end of the scale (10) corresponds to the “worst pain ever experienced or imagined.” For the classification of the pain, we considered mild pain: 5 mm to 44 mm; moderate pain: 45 mm to 74 mm; and severe pain: 75 mm to 100 mm.\n 28\nThe statistical analysis was performed using the 2016 version of the SAS University (SAS Institute, Inc., , Cary, NC, US) software,\n 29 \nand the results were shown as mean ± standard deviation or median and variation according to the distribution. At first, an exploratory data analysis was performed using the measurements of central position of dispersion and normality graphs. Given the asymmetry of the distribution of some variables, the non-parametric Mann Whitney test was used to compare the distribution of the quantitative variables in relation to the study groups.\n\nIn total, 122 women clinically diagnosed with CPP were invited to participate in the study. Of them, 96 took part in the evaluation, 5 of them were excluded because they were at menopause, a period that interferes in the %BF, and 91 women remained: 45 were diagnosed with CPP secondary to other causes (the group was named CPP no endometriosis), and 46 women were diagnosed with CPP secondary to endometriosis (the group was named CPP endometriosis). The recruitment and evaluation took place from April 2014 to October 2015 (\n Fig. 1 \n).\nRecruitment of the evaluated women with and without endometriosis.\nTable 1 \nshows the description and comparison between both groups in relation to the demographic variables.\nAbbreviations: CPP, chronic pelvic pain; SD, standard deviation.\nNote: *\n p \n-values: non-parametric Mann Whitney test.\nThe comparison of the demographic variables (age, marital status, schooling, profession) between the two groups did not reveal a significant difference. It is important to notice that in both groups there is a high prevalence of overweight women or women with with grade-I obesity, whereas only 20% of both groups present a nutritional-status classification as eutrophic, in other words, appropriate according to the BMI. To classify the nutritional status of the women in both groups, the BMI and the %BF assessed by the electrical bioimpedance test were measured.\nWe observed that both groups had similar results regarding these variables, with no significant difference between them (\n Table 2 \n).\nAbbreviation: CPP, chronic pelvic pain.\nRegarding the anthropometric variables presented in\n Table 3 \n(BMI, arm circumference, waist circumference, abdominal circumference, hip circumference, WHR and %BF), no difference among the women with CPP secondary to endometriosis was found.\nAbbreviations: AbP, abdominal perimeter; AP, arm perimeter; BMI, body mass index; HP, hip perimeter; WHR, waist-to-hip ratio; WP, waist perimeter.\nNote:\n p \n-values: non-parametric Mann Whitney test.\nIn relation to the pain in the VAS, which was evaluated in both groups, there was a significant difference (\n p \n < 0.05) between them (\n Table 4 \n).\nNote:\n p \n-values: non-parametric Mann Whitney test.\n\nIn the present study, we performed an analysis of body composition using anthropometric markers, evaluating the %BF compared with the intensity of clinical pain in women with a clinical diagnosis of CPP secondary to endometriosis and secondary to other causes. The groups were homogeneous in relation to the number of women evaluated and showed no difference in relation to the analyzed parameters and body composition.\nA study\n 7 \ndemonstrated that women with endometriosis have physical characteristics of BMI below the classification that is considered for the healthy population: they are often thin, and do not present obesity in relation to the control subjects. Nevertheless, we observed in the present study that both groups showed a percentage of women with BMI above what is considered appropriate. However, the BMI does not necessarily represent an increase in adiposity, since it represents the total body mass, and not only adipose tissue mass, not reflecting the distribution of the %BF.\n 30\nGurian et al,\n 31 \nin their study, aimed to analyze anthropometric parameters (BMI and %BF), and the clinical and experimental pain in women with CPP; the study revealed that a large part of the evaluated women presented a very high %BF, in other words, risk of disease associated with obesity,\n 32 \nas shown in the present study.\nAccording to and Ley et al,\n 33 \nthe mean gain in body weight at perimenopause is estimated between 2 kg to 4 kg in 3 years, with an increase of 20% in total body fat. Therefore, as in the present study, the mean age of the women evaluated corresponds to the perimenopause phase, which can at least partly explain our results.\nThe %BF in women with CPP shown in the present study was above the health levels recommended by the World Health Organization (WHO).\n 23 \nAlthough there are few studies on body composition related to women with CPP and their secondary causes,\n 31 \nsuch as endometriosis, we can understand that this increased percentage is probably due to certain factors, such as sedentary lifestyle, eating habits, metabolic alterations, and genetic predisposition.\n 13\nSeveral studies suggest that the adipose tissue has immune properties\n 10 \nand differs depending on the type and location of the tissue,\n 34 \nand that these properties may be involved or affected by the development of endometriosis.\n 9 \nWomen with endometriosis particularly present small amounts of adipose tissue (body fat) and adipose tissue below the waist (WHR). In the study by Shah et al,\n 35 \nthe waist circumference values showed a low relationship with endometriosis, and there was still no association between WHR and endometriosis. However, they suggest that a WHR below the recommended value may be related to up to a three-time higher chance of receiving the diagnosis of endometriosis. According to the present study, the WHR values between the groups did not show any difference, and this was the only ratio evaluated in the study.\nNevertheless, being thin is associated with the predominance of macrophages M2, whereas being overweight or obese is associated with the predominance of macrophages M1 (which promote inflammation and inhibit angiogenesis and tissue remodeling).\n 36 \nWe observed in the present study that, despite the fact that both groups had CPP, the endometriosis group showed a high %BF, which was a finding that differed from those in the literature, and they also presented values higher than what is indicated for the region below the waist. Obesity is associated with significant increases in morbidity and mortality; obese patients are more prone to develop diseases, such as hypertension, dyslipidemia, type-II diabetes mellitus, coronary heart disease, cerebral vascular accident, pulmonary dysfunction, among other chronic diseases, as well aggaravation of the endometriosis. An in-depth investigation of the aspects of adiposity with endometriosis is necessary to identify etiological or mediating pathways between them.\n 7\nVisscher et al\n 37 \nreported that the intensity of the pain has a great importance in the clinical practice, and it is recognized as one of the most relevant subjective pain experiences. The VAS is among the most widely used pain scales that aims to check the efficacy of the therapeutics and to compare the efficacy of different active treatments. It showed to be effective in the evaluation of the result of the treatments proposed in some randomized controlled trials applied to women with CPP of diverse causes; in them, the VAS was used as the primary outcome, as in the present study.\n 38\nThe main limitation of the present study is the women evaluated in the group without endometriosis who had different etiologies and comorbidities, which were not considered separately, but we do not believe that this compromised the results.\n\nWe conclude that there was no difference in relation to body composition and the anthropometric parameters evaluated in both groups of patients with CPP (with or without endometriosis); however, the group of patients with endometriosis showed higher pain scores than the patients without endometriosis.","source_license":"CC0","license_restricted":false}