{"paper_id":"a18838b2-4827-4aa1-a50c-730af566b69f","body_text":"Endometriosis is a benign gynecologic condition affecting 10% of women and characterized by the presence of extra-uterine endometrial tissue ( 1 – 3 ). The malignant potential of endometriosis is well-documented ( 4 – 6 ).\nThe clinical features and pathogenesis of endometriosis-associated ovarian cancer are topics of active investigation. Previous studies have documented an association between endometriosis-associated ovarian cancer and younger, premenopausal women who present with lower stage and tumor grade ( 5 ,  7 ,  8 ). Endometrioid and clear cell histologies are more common in endometriosis- associated ovarian cancer ( 5 ,  9 ,  10 ).\nPrior studies have reached discrepant conclusions regarding the impact of endometriosis on the survival of patients with ovarian cancer ( 5 ,  9 ,  11 ,  12 ). In a meta-analysis including 444,255 patients, there was no difference in progression free or overall survival between ovarian cancer patients with and without endometriosis ( 5 ). While some studies support these findings, other studies report improved survival among patients with endometriosis-associated ovarian cancer ( 5 ,  7 – 9 ,  11 – 13 ). Given these conflicting findings, we sought to characterize ovarian cancers associated with endometriosis and to evaluate the prognostic impact of the presence of endometriosis on ovarian cancer in our institution.\n\nOvarian cancer cases from Weill Cornell Medical College diagnosed between January 1, 2000 and December 31, 2013 were examined and specimens reviewed by two pathologists for the presence of endometriosis. Ovarian cancer cases were stratified by the presence or absence of endometriosis and were compared to determine the clinical factors associated with endometriosis and the prognostic significance of the presence of endometriosis. Institutional review board was obtained from Weill Cornell Medical College.\nClinical and pathologic data including year of diagnosis, age at diagnosis, tumor stage, tumor grade, pelvic or abdominal distribution of disease, and histology were collected. All patients underwent surgical evaluation. Adjuvant chemotherapy drugs and dates of administration were collected. Dates of recurrence and death were collected. Platinum free interval was calculated as the number of months from completion of platinum-based chemotherapy to recurrence. Progression free survival was calculated as the number of months from cancer diagnosis to recurrence, and overall survival was calculated as the number of months from cancer diagnosis to death. Patients who were alive at last follow-up or who had not recurred at last follow-up were censored.\nEach outcome was initially reported descriptively. Continuous variables were compared using two-sample T-tests. Frequency distributions between categorical variables were compared using χ 2  tests. Pearson Correlation Coefficients were calculated to determine trends in endometriosis associated ovarian cancer over time. Logistic regression models described predictors of association with endometriosis, while controlling for other predictive variables. Kaplan Meier curves were generated for platinum free interval, progression free survival, and overall survival based on association with endometriosis. Cox proportional hazards models were used to examine survival, comparing ovarian cancer cases with and without endometriosis. Outcomes were reported as hazards ratios and 95% confidence intervals. All statistical tests were two-sided and a p<0.05 was considered statistically significant.\n\nAmong 139 epithelial ovarian cancers diagnosed between 2000 and 2013, there were 49 (35%) associated with endometriosis and 90 (65%) not associated with endometriosis. The demographic characteristics and clinical variables of the study population are outlined in  Table 1 . There was no trend in incidence of ovarian cancer associated with endometriosis over time (Pearson Correlation Coefficient = 0.087, p=0.77). Ovarian cancer patients with endometriosis were younger than those without endometriosis (p<0.0001), with a median age among those with endometriosis of 52 years (47–57 years) and without endometriosis of 61 years (55–70 years). A woman was 7% less likely to have ovarian cancer associated with endometriosis for each year she aged. The distribution of histology of endometriosis-associated cancers included 21 (43%) endometrioid, 11 (23%) clear cell, 10 (20%) mixed, 4 (8%) mucinous, and 3 (6%) serous.\nOptimal surgical debulking was performed in 90% of endometriosis-associated cancers and in 79% of cases without endometriosis. The distribution of surgical approach for ovarian cancer patients with endometriosis was 34 (70%) laparotomy, 4 (8%) laparoscopy, 6 (12%) robotic, and 5 (10%) unknown, compared to patients without endometriosis, which were 71 (79%) laparotomy, 6 (7%) laparoscopy, 5 (5%) robotic, and 8 (9%) unknown. Patients with endometriosis were significantly more likely to be of early surgical stage (I/II vs. III/IV) compared to cases without endometriosis (79% vs 30%, respectively, P<0.0001). The tumor was confined to the pelvis in 89% of endometriosis associated cancers compared with 41% of cancers without endometriosis, (P<0.0001) Younger age ( P =0.0011), early stage ( P <0.0001), lower grade (p=0.014), and tumor confinement to the pelvis (p<0.0001) were all found to be independent predictors of the presence of endometriosis. Ovarian cancers diagnosed at early stage were eight times more likely to be associated with endometriosis compared to late stage (OR 7.646; 95% CI, 3.199 to 18.277). Among ovarian cancer cases associated with endometriosis, 67% received adjuvant chemotherapy, in contrast to 76% of cases not associated with endometriosis that received adjuvant chemotherapy.\nTable 2  displays Cox proportional hazards models of survival for ovarian cancer based on presence of endometriosis. Ovarian cancer patients with endometriosis had improved platinum free interval (HR=0.25; 95% CI, 0.12 to 0.55), progression free survival (HR=0.20; 95% CI, 0.09 to 0.43), and overall survival (HR=0.18; 95% CI, 0.04 to 0.81) compared to patients without endometriosis. Kaplan-Meier analysis confirmed the improved progression free (p<0.0001) and overall survival (p=0.012) experienced by patients with endometriosis-associated ovarian cancers and is shown in  Figures 1  and  2 , respectively. However, after controlling for tumor stage and age at diagnosis, endometriosis was not an independent predictor of survival.\n\nOur findings indicate that endometriosis is frequently associated with ovarian cancer and that this association is strongest in younger women with low tumor stage and grade. While an improved progression-free and overall survival was found in our patients with endometriosis-associated ovarian cancer, this benefit was related to the favorable pathologic features of the ovarian cancers and the presence of endometriosis had no independent impact on patient outcome. These findings are consistent with results of prior studies in this area.\nSimilarly to our findings, multiple studies report endometriosis associated ovarian cancer more commonly in younger women or premenopausal compared to postmenopausal women ( 8 ). Scarfone  et al  noted that compared with clear cell ovarian cancers without endometriosis, those with endometriosis occurred in younger women 51.4 years old vs. 58.4 years old (p=0.02) ( 13 ). In agreement with our study, in a meta-analysis, compared to ovarian cancers without endometriosis, those with endometriosis were more likely to be stage I-II (RR 1.959, 95% CI 1.367 to 2.807) and grade 1 (RR 1.319, 95% CI 1.149 to 1.514) ( 5 ). While many studies report that endometriosis associated ovarian cancers are most often clear cell or endometrioid histology ( 5 ,  11 ,  14 ), some studies also report the presence of endometriosis in low-grade serous ( 14 ) and mixed tumors ( 11 ). In an analysis of 15 published reports, the distribution of histologies among endometriosis associated ovarian cancers were clear cell (39.2%), endometrioid (21.2%), serous (3.3%), and mucinous (3.0%) ( 10 ).\nOverall prior studies comparing ovarian cancers with and without endometriosis show either no difference in survival, or improved either progression free or overall survival among cancers with endometriosis that is not statistically significant in multivariate analysis. Among 144 ovarian cancer patients, Cuff  et al  found no difference in progression free survival (p=0.7) ( 11 ), a finding similar to that of Scarfone  et al  who showed no difference in overall survival between these groups in a cohort of 73 patients ( 13 ). In a study of 201 patients, Davis  et al  demonstrated that compared to ovarian cancer patients without endometriosis, those with endometriosis had an improved 5-year progression free survival 75% vs. 55% (p=0.03) but no difference in overall survival 85% vs. 77% (p=0.2) ( 9 ). Noli  et al  and Garrett  et al  both demonstrated improved survival in univariate analysis that was not statistically significant in multivariate analysis ( 8 ,  12 ). In a meta-analysis of 444,255 patients, in crude analysis endometriosis associated ovarian cancer was associated with an improved overall survival (HR 0.778; 95%CI, 0.655–0.925) but not progression free survival (HR 1.023, 95% CI 0.712–1.470) compared to non-endometriosis associated ovarian cancer. In subgroup analyses, progression free survival and overall survival were not different between the groups ( 15 ).\nThe increased risk of ovarian cancer in women with endometriosis has been previously demonstrated. In a study of 20,686 women, endometriosis increased ovarian cancer risk (HR 1.9; 95% CI, 1.3 to 2.8); the increased risk of ovarian cancer was most notable in women with long-standing endometriosis greater than ten years ( 16 ). Similarly, in a pooled analysis of thirteen case control studies, including 13,226 controls and 7,911 ovarian cancer cases, the Ovarian Cancer Association Consortium noted that women with a reported history of endometriosis had an increased risk of clear cell ovarian cancer (OR 3.05, 95% CI 2.43–3.84), low grade serous ovarian cancer (OR=2.11; 95% CI, 1.39 to 3.20), and endometrioid ovarian cancer (OR=2.04; 95% CI, 1.67 to 2.48) ( 14 ). These findings call into question the safety of expectant management in long-standing ovarian endometriomas, particularly in perimenopausal women.\nThe favorable phenotype of endometriosis-associated ovarian cancer seen in the current study suggests a unique pathogenesis of these tumors. Recent advances in gene expression profiling and analysis have expanded our understanding of the genetic alterations associated with the various histologic types of ovarian cancer. Ovarian cancers that originate from malignant transformation of endometriosis appear to be part of type I ovarian cancers, which are typically of endometrioid or clear cell histology, with defects in ARID1A. These tumors are slower growing and more indolent compared to type II ovarian cancers ( 17 – 21 ). This genetic difference may explain the tendency for early stage and confinement to the pelvis in cancers associated with endometriosis in the current study. Both endometriosis and ovarian cancer have also been associated with alterations in the PIK3/AKT pathway ( 2 ,  22 ). Early clinical trials of novel inhibitors of PIK3 and its downstream effector, mTOR, in recurrent endometrial cancer have shown promising results and this may prove to be an effective strategy in endometriosis-associated ovarian cancer as well ( 23 ,  24 ). The relative resistance to platinum-based chemotherapy in low-grade serous and clear cell carcinoma, which are both associated with the presence of endometriosis, has been previously described ( 25 – 29 ). Future studies are needed to further elucidate the hormonal, inflammatory, and molecular pathways leading to the malignant transformation of endometriosis to ovarian cancer. Understanding of these pathways could identify precursor lesions in pelvic endometriosisthat provide a possible means for cancer risk stratification and identify a population that benefit from risk-reducing interventions.\nLimitations of the current study include the relatively small number of endometriosis-associated ovarian cancers, its retrospective study design, and the collection of all cases from a single institution. Larger, multi-institutional studies in this area are necessary to confirm our findings. While our study did not identify an independent impact of endometriosis on ovarian cancer survival, it adds to the literature supporting the role of endometriosis in identifying a specific phenotype of ovarian cancer defined by clear cell, and endometrioid histology, lower tumor grade, early onset, and confinement to the pelvis. As the progression toward a more individualized approach to ovarian cancer treatment continues, this information may prove useful in tailoring the surgical and medical approach to these tumors.","source_license":"CC0","license_restricted":false}