{"paper_id":"9fad2e2d-2d22-47c9-b461-4ffb3a2d985c","body_text":"Abstract\nUterine endometrial neovascularization during the menstrual cycle is regulated by a basic fibroblast growth factor (FGF) in the endometrium, consisting of endothelial and stromal cells. Acidic FGF (FGF-1), basic FGF (FGF-2) and hst-1 (FGF-4) proteins also possess angiogenic potency in vivo. Therefore, it is important to improve our understanding of the role of stromal cells in FGF supply for endometrial neovascularization. In this study, we determined FGF-1,-2, and -4 mRNA and FGF-2 by reverse transcription-polymerase chain reaction-Southern blot analysis, and enzyme-linked immunosorbent assay, respectively, in fibroblasts derived from uterine endometria as a substitute for stromal cells. Experimental results indicate that estradiol significantly increases the levels of intracellular and secreted FGF-2 and its mRNA expression in the FGF family in the fibroblasts. Moreover, progesterone reduces the estradiol-induced increase. Therefore, endometrial neovascularization might be partially regulated by stroma-derived FGF-2 under the influence of sex steroids through a paracrine cell-to-cell interaction.\nSimilar content being viewed by others\nReferences\nArcari P, Martinelli R, Salvatore F. The complete sequences of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: Evidence for multiple mRNA species. Nucleic Acids Res 12:9179–9189;1984.\nBradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:315–323;1976.\nChiu I-M, Wang W-P, Lehtoma K. Alternative splicing generates two forms of mRNA coding for human heparin-binding growth factor 1. Oncogene 5:755–762;1990.\nChomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159;1987.\nCullinan K, Koos RD. Expression of basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and keratinocyte growth factor (KGF) in the rat uterus-stimulation by estrogen. Biol Reprod 44(suppl):A536;1991.\nDenekamp J. Vascular as a target for tumor therapy. Prog Appl Microcirc 4:28–38;1984.\nEnenstein J, Waleh NS, Kramer RH. Basic FGF and TGF-β differentially modulate integrin expression of human microvascular endothelial cells. Exp Cell Res 203:499–503;1992.\nFolkman J, Haudenschild C. Angiogenesis in vitro. Nature 288:551–556;1980.\nFujimoto J, Hori M, Ichigo S, Tamaya T. Expressions of basic fibroblast growth factor and its mRNA in uterine endometrium during menstrual cycle. Gynecol Endocrinol, in press.\nKurokawa T, Sasada R, Iwane M, Igarashi K. Cloning and expression of cDNA encoding human basic fibroblast growth factor. FEBS Lett 213:189–194;1987.\nMarkee JE. Menstruation in intraocular endometrial transplants in the Rhesus monkey. Obstet Gynecol 131:558–559;1978.\nMignatti P, Mazzieri R, Rifkin DB. Expression of the urokinase receptor in vascular endothelial cells is stimulated by basic fibroblast growth factor. J Cell Biol 113:1193–1201;1991.\nMuthukrishnan L, Warder E, McNeil PL. Basic fibroblast growth factor is efficiently released from a cytosolic storage site through plasma membrane disruptions of endothelial cells. J Cell Physiol 148:1–16;1991.\nRandall LL, Hardy SJS. Unity in function in the absence of consensus in sequence: Role of leader peptides in export. Science 243:1156–1159;1988.\nRusnati M, Casarotti G, Pecorelli S, Ragnotti G, Presta M. Basic fibroblast growth factor in ovulatory cycle and postmenopausal human endometrium. Growth Factors 3:299–307;1990.\nSchreiber AB, Kenny J, Kowalski W, Friesel R, Mehlman T, Maciag T. Interaction of endothelial cell growth factor with heparin: Characterization by receptor and antibody recognition. Proc Natl Acad Sci USA 82:6138–6142;1985.\nTaira M, Yoshida T, Miyagawa K, Sakamoto H, Terada M, Sugimura T. cDNA sequence of human transforming gene hst and identification of the coding sequence required for transforming activity. Proc Natl Acad Sci 84:2980–2984;1987.\nAuthor information\nAuthors and Affiliations\nRights and permissions\nAbout this article\nCite this article\nFujimoto, J., Hori, M., Ichigo, S. et al. Ability of ovarian steroids to regulate the expression of the fibroblast growth factor family in fibroblasts derived from uterine endometrium. J Biomed Sci 3, 280–285 (1996). https://doi.org/10.1007/BF02253708\nReceived:\nAccepted:\nIssue date:\nDOI: https://doi.org/10.1007/BF02253708","source_license":"CC0","license_restricted":false}