{"paper_id":"962ae40c-1652-4fac-b537-2fd65e63a3f6","body_text":"Abstract\nCalcium ion (Ca2+) signaling in endometriosis (ENDO) is associated with increased neutrophil activation and oxidative stress. A Ca2+ signaling modulator and antioxidant actions of cabergoline (CBG) in some cells were recently reported. TRPM2 cation channel is activated by reactive oxygen species (ROS). Antioxidant action of CGB via inhibition of ROS may modulate the channel. We aimed to investigate the effect of CBG on TRPM2 inhibition in serum and neutrophils of patients with ENDO. The serum and neutrophil samples were grouped into healthy samples (no treatment), ENDO and ENDO + CBG treated groups (n = 10 in each). In some experiments, the neutrophils were also incubated with TRPM2 (ACA) and PARP-1 (PJ34) blockers. The values of intracellular ROS, Ca2+ concentration, mitochondrial membrane depolarization, lipid peroxidation, apoptosis, and caspase − 3, caspase − 9, PARP-1 and TRPM2 expressions were high in the neutrophils of patients with ENDO, although antioxidant levels (reduced glutathione, glutathione peroxidase, vitamin A, and vitamin E) were low in the neutrophils and serum from these patients. However, markers for apoptosis, oxidative stress, and mitochondrial dysfunction were reduced with CBG, ACA and PJ34 treatments, although the antioxidant levels were increased in the serum and neutrophils following treatment with CBG. Taken together, our current results suggest that CBG are useful antagonists against apoptosis and mitochondrial oxidative stress via inhibition of TRPM2 in neutrophils of patients with ENDO.\nSimilar content being viewed by others\nAvailability of data and materials\nThe raw data files may be available upon request or as a supplementary file if the paper is accepted.\nAbbreviations\n- ACA:\n-\nN-(p-amylcinnamoyl) anthranilicacid\n- ADPR:\n-\nADP-ribose\n- [Ca2+]i :\n-\nintracellular free Ca2+\n- CBG:\n-\ncabergoline\n- DMSO:\n-\ndimethyl sulfoxide\n- fMLP:\n-\nN-formyl-L-methionyl-L-leucyl-phenylalanine\n- Fura-2/AM:\n-\nFura 2-acetomethoxy ester\n- GPx:\n-\nglutathione peroxidase\n- GSH:\n-\nreduced glutathione\n- HBSS:\n-\nHank's buffered salt solution\n- LiPx:\n-\nlipid peroxidation\n- MDA:\n-\nmalondialdehyde\n- PARP-1:\n-\npoly ADP-ribose polymerase-1\n- ROS:\n-\nreactive oxygen species\n- SDU:\n-\nSuleyman Demirel University\n- TRP:\n-\ntransient receptor potential\n- TRPM2:\n-\ntransient receptor potential melastatin 2\nReferences\nBameri B, Shaki F, Ahangar N, Ataee R, Samadi M, Mohammadi H (2018) Evidence for the involvement of the dopaminergic system in seizure and oxidative damage induced by tramadol. 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Management of patients and blood collections form the patients were performed by EİE. MG made critical revision for the manuscript. All authors approved the final manuscript.\nCorresponding author\nEthics declarations\nConflict of interest\nWe declared no conflict of interest in relation to this article.\nEthics approval and informed consent\nThe study was approved by the local human ethic committee of Suleyman Demirel University (Protocol number: 2014-085. Date: 06.04.2014).\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nAbout this article\nCite this article\nEkici, E.İ., Güney, M. & Nazıroğlu, M. Protective effect of cabergoline on mitochondrial oxidative stress-induced apoptosis is mediated by modulations of TRPM2 in neutrophils of patients with endometriosis. J Bioenerg Biomembr 52, 131–142 (2020). https://doi.org/10.1007/s10863-020-09830-y\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s10863-020-09830-y","source_license":"CC0","license_restricted":false}