{"paper_id":"95a39173-3d8c-4749-b7f4-1ad335820e4a","body_text":"Abstract\nPatients with chronic pelvic inflammation (CPI) experience irregular menstrual, ectopic pregnancy, and infertility. Yiyi Baijiang Decoction attenuates CPI in patients with uncovered mechanisms. CPI therapeutic targets intersected with those of Yiyi Baijiang Decoction, followed by importing into STRING to obtain protein–target interaction. “Drug-component-disease-target” interaction was constructed by Cytoscape. mRNA and protein levels were detected by real-time quantitative PCR (RT-qPCR) and western blot. Yiyi Baijiang Decoction contained 199 active ingredients. There were 1071 drug targets for Yiyi Baijiang Decoction and 1622 therapeutic targets for CPI. The GO functional enrichment analysis revealed 3445 biological processes, and the KEGG pathway enrichment analysis screened 67 signal pathways. Decreased ALB, increased protein kinase B (AKT1), interleukin (IL)-6, vascular endothelial growth factor A (VEGFA), and phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K/AKT)-extracellular-regulated protein kinases (ERK)1/2 activation in CPI mice were abolished by Yiyi Baijiang Decoction. Yiyi Baijiang Decoction attenuates CPI by inactivating PI3K/AKT and ERK1/2 and regulating ALB, VEGFA, AKT1, and IL-6.\nSimilar content being viewed by others\nData Availability\nThe data used to support the findings of this study are available from the corresponding author upon request.\nReferences\nChen L, Ma F, Yu L (2022) Implementation of comprehensive service in nursing care of patients with chronic pelvic inflammation and evaluation of its effect on quality of life. 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All authors read and approved the final manuscript.\nCorresponding authors\nEthics declarations\nCompeting interests\nThe authors declare no competing interests.\nCompeting Interest\nAll authors have no financial, personal, or other relationships with others or organizations that could inappropriately influence the work.\nEthical Approval\nEthical approval for this study (SY2020-01) was provided by the Experimental Animal Ethics Committee of Hebei Ex&Invivo Biotechnology Co., Ltd on 10 February 2020.\nConsent for Publication\nWe confirm that the manuscript has been read and approved for publication by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed.\nInformed Consent\nThe present study followed international, China and Hebei Ex&Invivo Biotechnology Co., Ltd guidelines for humane animal treatment and complied with relevant legislation.\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nSupplementary Information\nBelow is the link to the electronic supplementary material.\nRights and permissions\nSpringer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.\nAbout this article\nCite this article\nWei, Y., Su, H., Li, J. et al. Exploration into the Mechanism of Yiyi Baijiang Decoction Attenuating Chronic Pelvic Inflammation Based on Network Pharmacology and Experimental Verification. Biochem Genet 63, 2879–2892 (2025). https://doi.org/10.1007/s10528-024-10843-0\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s10528-024-10843-0","source_license":"CC0","license_restricted":false}