{"paper_id":"8ad5a7b2-6ee0-4cff-9e79-68d27df5bdd6","body_text":"Abstract\nBackground\nThe nuclear factor kappa B (NF-κB) pathway is a critical mediator of regulated on activation, normal T cell expressed and secreted (RANTES) gene regulation and therefore represents a potential target for therapy of endometriosis-associated symptoms.\nObjective\nThe objective of this study was to investigate the effect of NF-κB decoy oligonucleotides (ODNs) on NF-κB activation, RANTES expression, and monocyte chemotactic activity in ectopic endometrial stromal cells in vitro.\nMethods\nA specific sandwich enzyme-linked immunosorbent assay (ELISA) was used to quantify RANTES expression in ectopic and normal endometrial stromal cells stimulated by interleukin (IL)-1β. Four hours after transfection of NF-κB decoy ODNs, 10 ng/ml IL-1β was added to induce the ectopic endometrial stromal cells to secrete RANTES. The NF-κB activation, RANTES expression, and monocyte chemotactic activity in ectopic endometrial stromal cells were respectively evaluated by electrophoretic mobility shift assay, ELISA, and Boyden chambers.\nResults\nIL-1β induced significantly higher levels (P < 0.05) of RANTES expression in a time-dependent manner in ectopic endometrial stromal cells compared with IL-1β-untreated ectopic and normal endometrial stromal cells. The RANTES accounts for the majority (68%) of the monocyte chemotactic activity in conditioned media of ectopic endometrial stromal cells. In vitro transfection of NF-κB decoy ODNs dramatically decreased (P < 0.05) the NF-κB activation, RANTES expression, and monocyte chemotactic activity in IL-1β-induced ectopic endometrial stromal cells.\nConclusions\nNF-κB decoy ODNs may exert anti-inflammatory effects in ectopic endometrial stromal cells via the suppression of NF-κB activation, RANTES expression, and monocyte chemotactic activity.\nSimilar content being viewed by others\nReferences\nHerrmann Lavoie C, Fraser D, Therriault MJ, Akoum A. Interleukin-1 stimulates macrophage migration inhibitory factor secretion in ectopic endometrial cells of women with endometriosis. Am J Reprod Immunol 2007;58(6):505–13. doi:10.1111/j.1600-0897.2007.00471.x.\nLebovic DI, Mueller MD, Taylor RN. Immunobiology of endometriosis. Fertil Steril 2001;75(1):1–10. doi:10.1016/S0015-0282(00)01630-7.\nNa YJ, Jin JO, Lee MS, Song MG, Lee KS, Kwak JY. 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Nuclear factor-kappa B decoy oligodeoxynucleotides attenuates ischemia/reperfusion injury in rat liver graft. World J Gastroenterol 2005;11(44):6960–7.\nAcknowledgments\nWe thank Dr. Lu Hua for the help in gel mobility shift assay.\nFunding\nThis work is supported by the National Natural Science Foundation of China No 30600671 (to Wang Xiu-li) and the Natural Science Foundation of Jiangsu province no BK2006577 (to Wang Xiu-li).\nAuthor information\nAuthors and Affiliations\nCorresponding author\nRights and permissions\nAbout this article\nCite this article\nXiu-li, W., Su-ping, H., Hui-hua, D. et al. NF-κB Decoy Oligonucleotides Suppress RANTES Expression and Monocyte Chemotactic Activity via NF-κB Inactivation in Stromal Cells of Ectopic Endometrium. J Clin Immunol 29, 387–395 (2009). https://doi.org/10.1007/s10875-009-9274-z\nReceived:\nAccepted:\nPublished:\nIssue date:\nDOI: https://doi.org/10.1007/s10875-009-9274-z","source_license":"CC0","license_restricted":false}