{"paper_id":"867b17c2-e753-40bf-989c-67db13c0f91b","body_text":"The World Health Organization defines abortion as “the expulsion or extraction from its mother of a fetus or embryo weighing 500 g or less” [ 1 ].\nCurrently, abortion is defined as a spontaneous loss of a pregnancy [ 1 ] before the fetal viability, which is considered to be before 20 weeks of gestation or when the fetus weighs 500 g or more [ 2 ].\nWorldwide, around 23 million pregnancy losses occur every year [ 3 ].\nSpontaneous abortion is one of the most common first-trimester complications, affecting over 15% of pregnant women of reproductive age [ 4 ].\nApproximately 50–70% of spontaneous conceptions are lost before the end of the first trimester; with most of them occurring early in pregnancy [ 5 ], primarily before 8–9 weeks of gestation [ 3 ].\nOverall, almost 11% of women experience at least one pregnancy loss, and 1.9% and 0.7% have two or three pregnancy losses, respectively [ 3 ].\nThe risk of pregnancy loss decreases with increasing gestational age, with fetal loss after 11 weeks being less than 3% [ 5 ].\nEPL can be theoretically divided into three categories based on etiology [ 4 , 6 ]: • Inevitable loss due to an intrinsically abnormal gestation (e.g., abnormal karyotype); • Failure to maintain an otherwise normal gestation due to underlying maternal physiologic or structural problems; • Pathologic elimination of a normal gestation due to an active maternal disease process (e.g., massive perivillous fibrin deposition, chronic histiocytic intervillositis, chronic villitis and decidual vasculitis).\nInevitable loss due to an intrinsically abnormal gestation (e.g., abnormal karyotype);\nFailure to maintain an otherwise normal gestation due to underlying maternal physiologic or structural problems;\nPathologic elimination of a normal gestation due to an active maternal disease process (e.g., massive perivillous fibrin deposition, chronic histiocytic intervillositis, chronic villitis and decidual vasculitis).\nThere are various possible causes of first-trimester miscarriage.\nOne of the most common risk factors for early pregnancy loss (EPL) is advanced maternal age.\nFor instance, the abortion rate for women aged 20–30 is 9% to 17%, but this rises to 80% at 45 years old [ 7 , 8 ].\nOther contributing factors include chronic maternal disease (e.g., diabetes, hyperprolactinemia, and autoimmune conditions), infections (e.g., syphilis, Zika virus and cytomegalovirus), and structural uterine abnormalities.\nModifiable lifestyle-related risk factors—such as alcohol consumption, smoking, cocaine use and exposure to environmental toxins—are also linked EPL [ 7 ].\nNearly 50–60% of early pregnancy losses are associated with fetal chromosomal abnormalities. The earlier the loss occurs, the more likely it is due to chromosomal defect [ 5 ].\nThere is a heterogeneous distribution of karyotype anomalies among these disorders [ 9 ]. Aneuploidy, defined as the presence of an abnormal number of chromosomes, are the main causes occurring in an early embryogenesis [ 3 , 10 ]; particularly, trisomies are the most common chromosomal abnormalities (50% to 52%), followed by monosomy X (15% to 24%), triploidy (15% to 22%), structural rearrangements (4% to 8%), and tetraploidy (2% to 7%) [ 5 , 9 ].\nChromosomal translocation and inversions are sporadic (2%) in sporadic miscarriage but are a possible cause of recurrent losses [ 5 ].\nEven though, in the majority of cases karyotype anomalies are de novo mutations and their impact on the risk in subsequent pregnancies is compound, the identification of the cause of abortion also plays an important role in genetic counseling [ 10 ].\nThe incidence of chromosomal abnormalities increases with maternal age, which contributes to age-related infertility [ 3 ].\nOther causes of EPL include endocrine and maternal diseases, anatomical abnormalities of the female genital tract, infections, immune factors, hereditary disorders and trauma [ 5 ].\nAssisted reproductive technology (ART) pregnancies (e.g., in vitro fertilization (IVF), Intracytoplasmic Sperm Injection (ICSI), egg donation) generally are characterized by a slightly higher risk of miscarriage compared to natural conceptions [ 11 ].\nAs with any pregnancy, the risk of spontaneous abortion (miscarriage) can occur in pregnancies conceived through ART, but there are several factors that may influence the risk, including the woman’s age, the quality of the embryos and whether the pregnancy is a singleton or multiple [ 11 ].\nThe miscarriage rate in ART pregnancies ranges from 15% to 25%. Whereas, in the general population, the risk of EPL is about 10–20%. Also in ART pregnancies, the maternal age is one of the most significant factors affecting the risk of miscarriage [ 12 ].\nWomen who undergo IVF are often older (>35 years) [ 13 ].\nIn fact, as women age, the quality of their eggs declines, which can lead to chromosomal abnormalities in the embryos, and this is one of the leading causes of miscarriage.\nFor women under 35, the miscarriage rate after IVF is typically around 15–20%.\nFor women over 40, the risk increases significantly, with some studies suggesting the rate could be 30–50% or higher, depending on factors like egg quality and the use of donor eggs [ 14 ].\nIn addition, embryos created through reproductive technology, especially from women of advanced maternal age, may have higher rates of aneuploidy leading to miscarriage. This is why genetic screening of embryos has become more common [ 15 ].\nAs in natural pregnancies, factors such as underlying maternal medical conditions (e.g., endometriosis and uterine abnormalities) and lifestyle factors (e.g., alcohol use, smoking, obesity) play important roles in determining the risk.\nFortunately, after a first pregnancy loss, many couples manage to have successful subsequent pregnancies; nevertheless, 2–4% of them will suffer recurrent losses, often without a recognized cause [ 16 ].\nThe histopathological examination of the abortive material is crucial for the diagnosis and for the subsequent management of a patient who experienced a first trimester miscarriage [ 17 ].\nThis procedure is useful in documenting an intrauterine or extrauterine pregnancy, identifying an important and unsuspected disease affecting the mother or the embryo and diagnosing conditions that may recur in future pregnancies.\nIn addition, the histology can explain the adverse fetal outcome and help the management of future pregnancies [ 4 ].\nA correct histopathological diagnosis of the possible cause plays and important role in the management and follow-up.\nIn some cases, this examination may be of some value in determining the possible causes of recurrent pregnancy loss, or it may show an unexpected pathology [ 1 ].\nIn summary, histopathological examination of first-trimester pregnancy loss is a valuable and often underutilized diagnostic tool. It enhances the clinician’s ability to determine the etiology of the loss, supports decision-making in subsequent pregnancies, provides reassurance and information to patients, and fulfills important legal documentation requirements. A structured and consistent approach to the pathological evaluation of EPL is essential for improving reproductive outcomes and patient care.\nThe aim of this paper is (1) to evaluate the histopathological features of the products of abortion in the first trimester; (2) to illustrate the major pathological findings according to the possible causes of the miscarriage; and (3) to propose a useful guide to the routine diagnosis.\nThe correct identification of the etiology of the pregnancy loss can give important prognostic and diagnostic recommendations to support future pregnancies.\nThe placenta plays a crucial role in nourishing the fetus, providing oxygen, removing waste products, and supporting the hormonal changes that maintain pregnancy; thus, its correct development is crucial.\nThe placenta undergoes several stages of development throughout the pregnancy.\nIts development begins around 5 to 6 days after fertilization, when the blastocyst implants in the uterine lining. The trophoblast—outer layer of the blastocyst—invades the endometrium and forms the chorionic villi, which represent the functional units of the placenta.\nDuring the first month, the trophoblast differentiates into two layers: the cytotrophoblast and the syncytiotrophoblast.\nBy week 4, a “primitive” placenta is formed.\nAround weeks 6–7, maternal blood perfuses the placenta via maternal vessels, enabling nutrient exchange.\nBy weeks 10–12, the placenta is fully functional and continues growing into the second trimester, reaching about 450 g at term [ 18 , 19 ].\nUmbilical cord development begins around the third week of embryogenesis with the formation of the connecting stalk, which links the embryo to the trophoblast. By week seven, the umbilical cord is fully developed, consisting of the connecting stalk, the vitelline duct, and the umbilical vessels, all encased in Wharton’s jelly and surrounded by the amniotic membrane [ 20 ].\nHistologically, the placenta in the first trimester (weeks 1–14) mainly consists of mesenchymal villi edematous and hypovascular—as well as a minimal number of immature intermediate villi [ 19 ] ( Figure 1 ).\n\nIn this retrospective cohort study, we analyzed abortive material from patients who experienced spontaneous first-trimester abortion between January 2012 and January 2025 at Agostino Gemelli Hospital, Rome, Italy.\nThe material was analyzed at the Pathological Department of the same Institution.\nAfter excluding 708 cases of recurrent abortion, a total of 1168 cases were included in the study.\nInclusion criteria were defined independently of patient demographics. No restrictions were applied regarding maternal age. With respect to gestational age, only first-trimester miscarriages (≤12 weeks of gestation) were considered. Case selection was based on histopathological adequacy: specimens were included if the sampled material had been entirely submitted for histological examination and was suitable for diagnostic evaluation. Specifically, eligibility required the presence of chorionic villi and maternal decidua, allowing a definitive histopathological diagnosis to be established.\nThe abortive material was received already fixed in 10% neutral buffered formalin. Upon arrival in the pathology laboratory, each specimen was macroscopically examined, appropriately sampled, and the weight of each sample was recorded. All available tissue was processed and embedded in paraffin to ensure complete histological evaluation. Paraffin blocks were then sectioned at approximately 5 µm thickness. The obtained sections were routinely stained with hematoxylin and eosin (H&E) for morphological assessment.\nIn selected cases, additional stains and immunohistochemical analyses were performed when clinically or morphologically indicated. In particular, in cases with suspicion of molar pregnancy, immunohistochemical staining for p57 was carried out to aid in the differential diagnosis. Similarly, in cases with histological features suggestive of cytomegalovirus infection, ancillary stains were performed as appropriate to support the diagnosis.\nAll cases were independently reviewed by two pathologists (VA and EN) and a joint review was performed for challenging cases. In cases of diagnostic uncertainty or discordance, the histopathological evaluation was re-assessed by the two pathologists (VA, EN) in a collegial manner. The re-evaluation process was initiated whenever one of the two observers had doubts regarding the initial histopathological interpretation. Each case was first independently reviewed in a blinded fashion with respect to the initial diagnosis. Subsequently, a joint review and discussion were performed to reach a consensus and establish the final diagnosis.\n\nThe patients’ mean age was 35.8 years, and none had a prior history of recurrent pregnancy loss. The mean gestational age of the examined cases was 9 weeks and 3 days.\nHistological findings recorded in the database enabled the formulation of a diagnostic hypothesis, categorizing the possible causes of miscarriage as follows ( Figure 2 ).\nKaryotype alterations can involve either the number or the structures of the chromosomes thus affecting normal fetal development and viability, often leading to spontaneous abortion.\nCommon numerical abnormalities include aneuploidy, trisomy, and monosomy; instead, structural chromosomal abnormalities include deletions, duplications, translocations, and inversions [ 21 ].\nWomen with a history of recurrent pregnancy loss may be more likely to have a chromosomal abnormality in their embryos. In these cases, chromosomal testing of the products of conception may help identify the underlying cause.\nAmong our cases, 40.2% showed histological features suggestive of karyotype alterations. In particular, in 27.8% of cases, the presence of a chromosomal abnormality was genetically confirmed (primarily triploidy). In the remaining cases, although no genetic confirmation was available, the histological characteristics did not allow allocation of the cases to any of the other categories.\nPolymorphic, edematous and hypo/avascular chorionic villi with pseudo-cystic degeneration. Amniochorionic fragments, fetal red blood cells (RBCs), and necrotic decidua may be present.\nAll these findings are suggestive, but not conclusive, of karyotype alterations.\nA genetic test is then recommended ( Figure 3 A,B).\nTrophoblast implantation defects (TID) can cause an early pregnancy loss. These defects occur when the trophoblast cells fail to implant properly into the endometrium leading to insufficient placental development [ 22 ].\nSeveral factors can cause a defective implantation including endometrial chronic inflammation or infections, autoimmune disorders (e.g., lupus or antiphospholipid syndrome), hormonal imbalances (particularly inadequate progesterone production), environmental factors (e.g., smoking, alcohol use, and obesity) and advanced maternal age.\nNormal villar morphology with poor trophoblast invasion and retention of muscular vessels layers in the decidua suggests implantation failure ( Figure 3 C).\nAbruptio placentae is a condition that can result in pregnancy loss, particularly in the later stages of gestation. It refers to a premature detachment of the placenta before delivery of the fetus caused by bleeding at the decidual placental interface [ 23 ]. Placental abruption occurs in 0.4% to 1.0% of all pregnancies [ 24 ]. This detachment deprives the fetus of oxygen and nutrients and potentially leads to pregnancy loss, preterm birth, and maternal hemorrhage [ 25 ]. Different risk factors can lead to placental abruption such as: abdomen trauma, preeclampsia, previous placental abruption, cocaine use, multiple pregnancy, advanced maternal age, and infection (e.g., chorioamnionitis) [ 25 ].\nIschemic villi, intervillous blood, fibrin, and thrombi are in the decidua.\nInflammatory infiltrates suggest a secondary inflammatory etiology ( Figure 3 D–G).\nA blighted ovum, also known as anembryonic pregnancy, is a possible cause of early pregnancy loss and it is characterized by a gestational sac that forms and grows while an embryo fails to develop or stops developing early in the pregnancy [ 26 ]. The exact cause of a blighted ovum is still not clear, but it is believed to be due to chromosomal abnormalities or genetic errors that occur during fertilization. A transvaginal ultrasound is the most reliable way to diagnose a blighted ovum where no embryo is visible in the gestational sac.\nEdematous chorionic villi with irregular contours and thinned trophoblast. The gestational chamber has a thinned amniotic lining in the absence of fetal red blood cells and embryonic residues.\nExuberant placental site is a rare condition in which abnormal placental tissue remains and grows in the uterus after a pregnancy has ended, leading to different complications and adverse outcomes [ 27 ]. This can occur after any pregnancy, but it is most associated with molar pregnancies. If an exuberant placental site is left untreated or if it evolves into gestational trophoblastic disease (GTD), it can lead to pregnancy loss or significant complications in subsequent pregnancies.\nPolymorphic and polymetric as well as edematous and hydropic chorionic villi. Decidua flaps show smooth muscle fibers, referable to the myometrium, mixed with elements of extravillous trophoblast ( Figure 4 G).\nThough benign, follow-up is required.\nA partial molar pregnancy (PMP) is a gestational trophoblastic disease that can cause EPL due to abnormal development of both the placenta and fetus. It is characterized by a triploid karyotype [ 28 ]. The placenta tissue grows abnormally, forming cysts, and cannot properly support the fetus. The lack of adequate placental support typically leads to miscarriage.\nPolymorphic and polymetric chorionic villi with deep incisions in the trophoblastic lining and focal non-polar proliferation, pseudo-cystic degeneration, and presence of stromal cisterns. A second population of small and fibrotic villi is present. Fetal elements may be seen ( Figure 4 A,B).\nImmunostaining for p57 (nuclear expression) is positive in at least 10% of villous stromal cells and cytotrophoblasts ( Figure 4 E).\nA complete molar pregnancy (CMP) is a gestational trophoblastic disease that almost always leads to pregnancy loss during the first trimester. It occurs when there is an abnormal fertilization of an egg, resulting in an abnormal growth of trophoblastic tissue [ 29 ].\nThe abnormal proliferation of the trophoblastic cells leads to the formation of large fluid-filled cysts. Thus, the placenta cannot carry out its normal functions of nutrient and oxygen exchange, leading to inadequate placental support for any potential fetal development.\nIn addition, unlike a partial molar pregnancy, a complete molar pregnancy typically has no fetal development.\nWith proper treatment and follow-up, the prognosis is generally excellent, and women can go on to have future pregnancies [ 30 ].\nFestooned villi, circumferential trophoblast proliferation, stromal cisterns, and nuclear atypia are identified. No embryo develops ( Figure 4 C,D).\nImmunostaining for p57 (nuclear expression) is negative in cytotrophoblast and villous stroma ( Figure 4 F).\nIn some cases, histopathological evaluation of decidual–ovular tissue revealed the presence of embryonic components ( Figure 4 H).\nIn  Table 1  are reported the most frequent causes of first-trimester miscarriage with their prevalent histopathological findings and reports.\n\nEarly pregnancy loss (EPL) remains one of the most frequent and distressing complications in the first trimester of pregnancy, representing a major clinical concern for gynecologists and a significant emotional burden for patients [ 1 ]. While many early miscarriages—particularly those caused by chromosomal anomalies—are biologically unavoidable, identifying the underlying cause of EPL is essential for guiding future clinical management, patient counseling, and psychological support.\nHistopathological examination plays a pivotal role in the diagnostic evaluation of EPL. It serves multiple purposes: confirming the presence of intrauterine pregnancy, distinguishing gestational from non-gestational tissue, and identifying critical maternal or embryonic pathologies. These include infections, molar pregnancies, chronic inflammatory conditions (e.g., chronic villitis or chronic histiocytic intervillositis), and placental abnormalities such as massive perivillous fibrin deposition or decidual vasculopathy, which may otherwise go undetected. Recognizing such abnormalities is particularly important in cases of recurrent pregnancy loss, where targeted interventions may be required. Moreover, histological assessment can uncover unsuspected maternal or gestational conditions that may not be clinically apparent but have prognostic significance for future pregnancies. For example, the detection of molar tissue necessitates follow-up for gestational trophoblastic disease, while identification of inflammation or vascular pathology may indicate underlying systemic disorders such as autoimmune disease or thrombophilia.\nThe value of histopathological analysis extends beyond diagnosis. It offers patients and providers concrete information regarding the nature of the loss, contributing to emotional closure and aiding in the grieving process. From a medico-legal standpoint, histological documentation provides objective evidence of intrauterine pregnancy and may help in the defense or clarification of medical decisions made during pregnancy management.\nDespite its importance, histopathological examination is not uniformly performed following EPL. This variability underscores the need for standardized guidelines recommending routine submission of products of conception for histological evaluation, especially in cases where the clinical diagnosis is uncertain, the pregnancy was achieved via assisted reproductive technology, or the patient has a history of recurrent losses.\nThe main strength of our study lies in the relatively large sample size of first-trimester pregnancy loss cases, which enhances the robustness of the morphological observations. Additionally, the histopathological evaluation was performed by two pathologists, with discordant or uncertain cases undergoing blinded re-evaluation followed by joint discussion, thus increasing diagnostic consistency and reliability.\nThe principal limitation of the study is the lack of correlation with clinical characteristics of the patients. However, it is important to emphasize that the primary objective of this work was to develop and propose a structured framework to guide histopathological reporting in first-trimester pregnancy loss. For this reason, the study was intentionally focused on morphological standardization rather than clinicopathological integration.\n\nEarly pregnancy loss, while often unavoidable, warrants thorough evaluation to guide future clinical care and offer patients clarity and support. Histopathological examination of first-trimester pregnancy loss plays a critical role in identifying intrauterine gestation, uncovering underlying pathological processes, and informing management strategies—particularly in cases of recurrent loss or high-risk pregnancies.\nBy integrating histology into routine diagnostic protocols, clinicians can improve the accuracy of EPL assessments, offer more personalized care, and better counsel patients regarding prognosis and recurrence risks.\nA structured and systematic histopathological approach should therefore be considered a standard component of EPL management, contributing meaningfully to diagnostic precision, patient outcomes, and reproductive planning.","source_license":"CC-BY-4.0","license_restricted":false}