{"paper_id":"85bd1306-a742-4fc2-8721-68cc85d18b35","body_text":"Abstract\nEndometrial-like stromal cells, one of the main components of endometriotic lesions, are an important in vitro model for studying cellular and molecular mechanisms associated with lesion development in endometriosis. However, the short life span of primary endometriotic stromal cells (Ec-ESCs) limits their use. Human telomerase reverse transcriptase (hTERT) plasmids can be used to develop immortalized cell lines. Here we aimed to establish an endometriotic stromal cell line by hTERT immortalization. Primary Ec-ESCs were obtained from a human ovarian endometriotic cyst. The purity was assessed by morphology and the expression of vimentin, cytokeratin, and human interferon-inducible transmembrane protein 1 (hIFITM1). Cells were infected with hTERT lentiviral vector and selected with hygromycin. hTERT mRNA levels were confirmed by RT-qPCR. Immortalized Ec-ESCs (iEc-ESCs) were characterized by examining the expression of morphological markers and key genes of interest, TP53, estrogen receptor β (ERβ), progesterone receptor (PR), and steroidogenic factor-1 (SF-1). Karyotyping and in vitro decidualization studies were also performed. Ec-ESCs were positive for vimentin and hIFITM1 and negative for cytokeratin, indicating that they were representative of Ec-ESC. The fibroblast-like morphology, expression of TP53, ERβ, PR, and SF-1 did not change before and after hTERT immortalization. iEc-ESCs showed an impaired decidualization response like primary Ec-ESCs when compared to normal eutopic stromal cells. Karyotyping showed that 15/19 cells had normal female karyotype, while 4/19 cells had partial trisomy 11q. Collectively, we successfully established and characterized an immortalized endometriotic stromal cell line. 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Endocrinology. 2008;149(3):1180–9.\nAcknowledgments\nThe authors thank Spectrum Health Universal Biorepository and Prisma Healthcare for the sample collection.\nFunding\nThis research was supported by NIH grants RO1 HD042280 and HD083273 to A.T.F.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nEthics declarations\nConflict of Interest\nThe authors declare that they have no conflict of interest.\nEthics Approval\nThis study has been approved by Michigan State University, Spectrum Health, and Prisma Healthcare Institutional Review Boards.\nConsent to Participate (Include Appropriate Statements)\nWritten informed consent has been obtained from the patients.\nConsent for Publication (Include Appropriate Statements)\nThe authors declared that the work has not been published in whole or in part elsewhere.\nAdditional information\nPublisher’s Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nElectronic supplementary material\nESM 1 (download DOCX )\n(DOCX 95 kb)\nRights and permissions\nAbout this article\nCite this article\nSong, Y., Joshi, N.R., Vegter, E. et al. Establishment of an Immortalized Endometriotic Stromal Cell Line from Human Ovarian Endometrioma. Reprod. Sci. 27, 2082–2091 (2020). https://doi.org/10.1007/s43032-020-00228-0\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s43032-020-00228-0","source_license":"CC0","license_restricted":false}