{"paper_id":"7f9becb9-1044-419e-a4bb-3c8508d4dc31","body_text":"Abstract\nEndometriosis is a common estrogen-dependent chronic disease of women of reproductive age; it is associated with dysregulation of the immune response, local inflammatory process and increased autoantibody production. The purpose of this study was to investigate the autoantibody profile in women with endometriosis by means of new modifications of the enzyme-linked immunosorbent assay and to evaluate their diagnostic value. In women with external genital endometriosis stage III–IV (n = 39), a wide range of autoantibodies of mainly class G was detected; these included antibodies to endometrial antigens (tropomyosin 3, tropomodulin 3), the enzyme α-enolase, steroid hormones (estradiol, progesterone) and gonadotropin. Moreover, the detection rate of IgG antibodies to tropomyosin 3, α-enolase, estradiol, and human chorionic gonadotropin and their levels in patients with endometriosis were higher than in healthy women (n = 26) (p < 0.05). IgG antibodies to tropomyosin 3, α-enolase and estradiol were characterized by a higher diagnostic value for endometriosis. When determining the combination of these antibodies, the diagnostic significance increased, the AUC value was 0.875 [0.772–0.978] (p < 0.0001), and sensitivity and specificity reached 83.3%. Thus, autoantibodies to tropomyosin 3, α-enolase and estradiol are promising for inclusion in the panel of biomarkers for non-invasive diagnosis of endometriosis.\nSimilar content being viewed by others\nREFERENCES\nBulun, S.E., Yilmaz, B.D., Sison, C., Miyazaki, K., Bernardi, L., Liu, S., Kohlmeier, A., Yin, P., Milad, M., and Wei, J., Endocrine Reviews, 2019, vol. 40, no. 4, pp. 1048–1079. https://doi.org/10.1210/er.2018-00242\nZhang, T., De Carolis, C., Wai Man, G.C., and Wang, C.C., Autoimmunity Reviews, 2018, vol. 17, pp. 945–955. https://doi.org/10.1016/j.autrev.2018.03.017\nLagana, A.S., Garzon, S., Gotte, M., Vigano, P., Franchi, M., Ghezzi, F., and Martin, D.C., Int. J. Mol. Sci., 2019, vol. 20, 5616. https://doi.org/10.3390/ijms20225615\nTosti, C., Pinzauti, S., Santulli, P., Chapron, C., and Petraglia, F., Reprod. Sciences, 2015, vol. 22, no. 9, pp. 1053–1059. https://doi.org/10.1177/1933719115592713\nKorotkova, T.D., Adamyan, L.V., Stepanyan, A.A., Krechetova, L.V., and Vanko, L.V., Problemy Reproduktsii, 2018, vol. 24, no. 6, pp. 22–31. https://doi.org/10.17116/repro20182406122\nDorien, F.O., Flores, I., Waelkens, E., and D’Hooghe, T., Best Practice Research Clinical Obstetrics Gynaecology, 2018, vol. 50, pp. 72–83.\nNisenblat, V., Bossuyt, P.M.M., Shaikh, R., Farquhar, C., Jordan, V., Scheffers, C.S., Mol, B.W.J., Johnson, N., and Hull, M.L., Cochrane Database Syst Rev., 2016, vol. 5, CD012179. https://doi.org/10.1002/14651858.CD012179\nGajbhiye, R., Bendigeri, T., Ghuge, A., Bhusane, K., Begum, S., Warty, N., Sawant, R., Padte, K., Humane, A., Dasmahapatra, P., Chauhan, A. and Khan, S., Reprod. Sciences, 2017, vol. 24, no. 3, pp. 413–420.https://doi.org/10.1177/1933719116657190\nNabeta, M., Abe, Y., Kagawa, L., Haraguchi, R., Kito, K., Ueda, N., Sugita, A., Yokoyama, M., Kusanagi, Y., and Ito, M., Proteomics Clin. Appl., 2009, vol. 3, pp. 1201–1210. https://doi.org/10.1002/prca.200900055\nNabeta, M., Abe, Y., Takaoka, Y., Kusanagi, Y., and Ito, M., J. Reprod. Immunol., 2011, vol. 91, pp. 48–55. https://doi.org/10.1016/j.jri.2011.04.012\nMenzhinskaya, I.V., Gladkova, K.A., Sidelnikova, V.M., and Sukhikh, G.T., Immunologiya, 2008, vol. 29, no. 1, pp. 34–37.\nMenzhinskaya, I.V., Kashentseva, M.M., Vanko, L.V., and Sukhikh, G.T., Immunologiya, 2015, vol. 36, no. 1, pp. 30–35.\nCappello, P., Principe, M., Bulfamante, S., and Novelli, F., Front. Biosci., 2017, vol. 22, pp. 944–959.\nACKNOWLEDGMENTS\nThe authors are grateful to the Laboratory for the Collection and Storage of Biological Material of Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology (Head, S.M. Mullabaeva).\nFunding\nThe work was performed within the State Assignment “Development of Approaches for Non-invasive Diagnostics of Endometriosis based on Omics Technologies,” registration no AAAA-A19-119021490132-9.\nAuthor information\nAuthors and Affiliations\nCorresponding author\nEthics declarations\nCOMPLIANCE WITH ETHICAL STANDARDS\nThe study protocol was approved by the Commission on Ethics of Biomedical Research (Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology). Before inclusion in the study, all participants gave written informed consent.\nCONFLICT OF INTERESTS\nThe authors declare that they have no conflict of intere-st.\nAdditional information\nTranslated by A. Medvedev\nRights and permissions\nAbout this article\nCite this article\nMenzhinskaya, I.V., Melkumyan, A.G., Pavlovich, S.V. et al. Autoimmune Markers for Non-Invasive Diagnosis of Endometriosis in Women. Biochem. Moscow Suppl. Ser. B 14, 335–339 (2020). https://doi.org/10.1134/S1990750820040083\nReceived:\nRevised:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1134/S1990750820040083","source_license":"CC0","license_restricted":false}