{"paper_id":"7e75f6d8-615d-4026-8cf9-b9fa34a7e1a0","body_text":"Abstract\nEndometriosis is a polygenic and multifactorial gynecology situation which might be associated with angiogenesis. In the current study we assess the role of vascular endothelial growth factor (VEGF) − 2578 A/C, and + 936 C/T polymorphisms in susceptibility to endometriosis and checking the expression of VEGF mRNA in eutopic tissue of endometrium with and without endometriosis. The study was comprised of 300 patients who underwent laparascopic or laparotomy surgery with 100 cases who had confirmed histological diagnosis of endometriosis, and 200 controls with no histological diagnosis of disease. The genotyping of VEGF polymorphisms was done by polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) technique and the gene expression in tissue was determined using Real-Time PCR assay. There was no important difference of allele distribution of the − 2578 A/C (P = 0.7) and + 936 C/T (P = 0.5) polymorphisms among endometriosis cases and controls. Study of VEGF expression during the menstrual cycle, showed that endometrial tissue in cases group expressed more VEGF mRNA at the secretory phase compared to the proliferative phase (P = 0.03). Our results suggest that − 2578 A/C and + 936 C/T polymorphisms of VEGF did not seem to have impact on endometriosis predisposition in our study population. Also we did not find any link between VEGF mRNA expression and risk of endometriosis.\nSimilar content being viewed by others\nReferences\nHsieh Y-Y, Chang C-C, Tsai F-J, Yeh L-S, Lin C-C, Peng C-T (2004) T allele for VEGF gene-460 polymorphism at the 5′-untranslated region: association with a higher susceptibility to endometriosis. 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In addition, laboratory work was done in Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences (TUMS) at Shariati Hospital. This work was supported by Vali-e-Asr Reproductive Health Research Centre, Tehran University of Medical Sciences.\nAuthor information\nAuthors and Affiliations\nCorresponding authors\nEthics declarations\nConflict of interest\nAll authors of original research article declare that they have no conflict of interest.\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nElectronic supplementary material\nBelow is the link to the electronic supplementary material.\nRights and permissions\nAbout this article\nCite this article\nRashidi, B.H., Sarhangi, N., Aminimoghaddam, S. et al. Association of vascular endothelial growth factor (VEGF) Gene polymorphisms and expression with the risk of endometriosis: a case–control study. Mol Biol Rep 46, 3445–3450 (2019). https://doi.org/10.1007/s11033-019-04807-6\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s11033-019-04807-6","source_license":"public-domain-us","license_restricted":false}