{"paper_id":"7e75b584-be1c-4236-a657-977816c5f669","body_text":"In July 2022, a previously healthy 69-year-old man (nature filmmaker) came to an outpatient clinic in Austria because of fever (temperature up to 39°C), chills, malaise, headache, and lower abdominal pain after traveling to southern Slovenia, Cres island (Croatia), and northern Styria (Austria) 1 month earlier. The patient reported several tick bites but no further animal contact. Clinical examination showed a small ulcerative lesion on the lower left back, which was initially suspected to be an infected insect bite.\nLaboratory tests showed leukocytosis (13.6 × 10 9  cells/L), increased C-reactive protein (CRP) of 85 mg/L, a serum creatinine level of 1.17 mg/mL, and an estimated low glomerular filtration rate of 63 mL/min/1.73m 2 . The patient was admitted and initially given amoxicillin/clavulanic acid, which was subsequently changed to piperacillin/tazobactam plus moxifloxacin 3 days later because of persistent fever and sudden testicular swelling and pain, as well as an increased CRP level (357 mg/L) and leukocyte count (22.5 × 10 9  cells/L).\nComputed tomography of the thorax, abdomen, and pelvis showed bilateral epididymo-orchitis and an enlarged right testicle with hyperperfusion and nonperfused areas and a hypoperfused left testicle ( Figure 1 ). In addition, a pulmonary infiltration (diameter 5 mm) in the left lower lobe and diverticulitis were detected. Results of blood and urine cultures were negative. Urine antigen test results for  Legionella  sp. and pneumococci showed negative results. Antibodies specific for  Brucella  spp.,  Leptospira  spp., and HIV were not detected.\nComputed tomography of patient who had human tularemia epididymo-orchitis caused by  Francisella tularensis  subspecies  holartica , Austria. Coronal image shows the right testicle (arrow) during the arterial phase with hyperperfusion and nonperfused areas (abscess).\nThe patient was transferred to the Medical University of Graz, where piperacillin/tazobactam was continued and moxifloxacin stopped. During the next few days, sonographic examinations showed an enlarged and inhomogenous and hyperperfused right testicle with clinical epididymitis but decreasing CRP levels. Persistent pain and progressive inflammation observed by testicular ultrasound, including suspected abscess, led to unilateral orchiectomy (right testicle) 14 days after admission. Intraoperatively, the testicle and the spermatic cord showed massive inflammation. The orchiectomy was performed without any complications.\nThe leukocyte count returned to the reference range, and the CRP level decreased to 68 mg/L after surgery. Histopathologic examination showed a chronic granulomatous epididymo-orchitis with abundant suppurative granulomas located between destroyed seminiferous tubules in addition to diffuse mixed interstitial inflammatory infiltrate ( Figure 2 ). PCRs results for  Mycobacterium tuberculosis  complex and atypical mycobacteria were negative. A 16S rRNA gene sequencing analysis of testicular tissue using an IonTorrent Platform ( https://www.thermofisher.com ) showed abundant bacterial DNA with 100% homology for  F. tularensis  subspecies  holartica  (78% of generated reads) ( 1 ).\nGranulomatous epididymo-orchitis in patient who had human tularemia epididymo-orchitis caused by  Francisella tularensis  subspecies  holartica , Austria. A) Diffuse chronic granulomatous inflammation in the interstitium and between the seminiferous tubule. Hematoxylin and eosin stain; original magnification ×20. B) Suppurative granuloma with epithelioid cells and single giant cells in testicular tissue. Hematoxylin and eosin stain; original magnification ×100.\nSubsequently, a commercially available ELISA (Virion/Serion,  https://www.virion-serion.de ) detected IgM and IgG for a panel of human pathogens in serum or plasma (cutoff value >15 units/mL for IgG and IgM) and indicated the presence of  F. tularensis  lipopolysaccharide.  F. tularensis  antibody levels were 136 units/mL for IgG and >400 units/mL for IgM. The patient received doxycycline plus moxifloxacin for 2 months. At a 6-month follow-up, the patient had no additional complaints.\n\nIn Austria, antibodies against  F. tularensis  are found in 0.5% of healthy adults ( 5 ), and annual cases range between 0 and 58 ( 2 , 6 ). In clinically apparent infections, the most frequent manifestations of human tularemia are ulceroglandular or glandular forms. The oculoglandular, oropharyngeal, or pulmonary forms have been less frequently reported ( 2 ). In the case we report, the patient did not report any direct animal contact or use of unprocessed water or food during his nature filming activities. However, initial examination showed a small ulcerative lesion on the lower left back, which presumably was the initial tularemia skin lesion.\nWorldwide, tularemia orchitis has been reported in hares (including 1 case with epididymo-orchitis) ( 7 , 8 ), a squirrel ( 9 ), and a marmoset ( 10 ). Infectious human epididymo-orchitis is usually caused by  Neisseria gonorrhoeae ,  Chlamydia trachomatis ,  Ureaplasma  spp.,  Mycoplasma genitalium ,  Escherichia coli ,  Pseudomonas aeruginosa , and other gram-negative bacteria, as well as  Staphylococcus aureus  in elderly persons. Granulomatous epididymo-orchitis is rare and usually caused by  Mycobacterium tuberculosis  or  Brucella  spp. Other rare etiologic agents include fungi,  Shistosoma  spp., or  Orientia tsutsugamushi  ( 11 – 13 ).  F. tularensis  has not been previously reported as a causative microorganism for epididymo-orchitis.\nPatients who have epididymo-orchitis typically have acute onset unilateral scrotal pain, swelling, and erythema, and treatment with ceftriaxone combined with doxycycline or levofloxacin is recommended ( 13 ). Tularemia is treated with fluorochinolones, doxycycline, or aminoglycosides depending on disease severity. Our patient empirically received piperacillin/tazobactam with moxifloxacin, but moxifloxacin was discontinued after 3 days. During piperacillin/tazobactam monotherapy, CRP levels decreased, but sonography and clinical status worsened, leading to unilateral orchiectomy. We assume that the empirical application of moxifloxacin for 3 days lowered systemic inflammatory parameters but was too short for improvement of the testicular infection. Ultimately, the etiology of epididymo-orchitis could be elucidated by 16S rRNA gene sequencing analysis, which in this case led to successful directed therapy with doxycycline and moxifloxacin. Because of impaired renal function, aminoglycosides were not considered. Furthermore, serologic analysis confirmed this unusual case of tularemia.\nThe specific source of tularemia in this case remains unknown because  F. tularensis  subsp.  holartica  was prevalent in all countries visited by the patient before his infection (tularemia cases are reported from Austria and Slovenia and, rarely, from Croatia) ( 14 ). Nevertheless, our findings indicates that, in patients suspected of having tularemia by medical history (e.g., arthropod bites, animal contact) or clinical examination (e.g., ulcer, rash, lymphadenopathy), clinicians should consider  F. tularensis  as a rare etiologic microorganism in epididymo-orchitis.","source_license":"CC-BY-4.0","license_restricted":false}