{"paper_id":"7bc0140a-d4b6-4cf4-8822-ae072658ed06","body_text":"Abstract\nHere, we determined basic differentially expressed genes in endometrial lesions compared to eutopic endometrium of the patients with endometriosis (EM). Endometrial biopsy and tissue sampling were performed in ten women with pelvic EM and nine controls at their mid-secretory phase. NGS of mRNA combined with bioinformatic analysis and complemented by RT-qPCR analysis were used for analysis. A list of differentially expressed genes of ectopic compared to eutopic endometrium during mid-secretary phase has been composed. Functional annotation has found profound gene expression perturbations in numerous EM pathways including extracellular matrix, complement and coagulation, as well as cell cycle-related gene family. The genes of alcohol dehydrogenase 1B (ADH1B) and fatty binding protein 4 (FABP4) were determined as the most dramatically up-regulated genes in ectopic endometrial lesions. Both genes were not reported before to be associated with EM. Several other genes including PLA2G2A were also up-regulated in ectopic EM lesions. Notably, FABP4 and ADH1B are key genes in basic metabolism and known for their function in adipose tissue. The results provide intriguing new avenue for endometriosis studies.\nSimilar content being viewed by others\nREFERENCES\nEndometriosis: Science and Practice, Giudice, L.C., Evers, J.L.H., and Healy, D.L., Eds., New York: Blackwell, 2012.\nEisenberg, V.H., Weil, C., Chodick, G., and Shalev, V., Epidemiology of endometriosis: a large population-based database study in a 2-million-member health care provider, BJOG, 2018, vol. 125, no. 1, pp. 55-62. doi 10.1111/1471-0528.14711\nSimoens, S., Dunselman, G., Dirksen, C, et al., The burden of endometriosis: costs and quality of life of women with endometriosis and treated in referral centres, Hum. 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Endocrinol., 2015, vol. 13, pp. 75-87. doi 10.1186/s12958-015-0063-7\nACKNOWLEDGMENTS\nThe work was supported by Russian Science Foundation (grant no. 14-15-00737).\nAuthor information\nAuthors and Affiliations\nCorresponding author\nAdditional information\nThe article is published in the original.\nRights and permissions\nAbout this article\nCite this article\nPredeus, A.V., Vashukova, E.S., Glotov, A.S. et al. Next-Generation Sequencing of Matched Ectopic and Eutopic Endometrium Identifies Novel Endometriosis-Related Genes. Russ J Genet 54, 1358–1365 (2018). https://doi.org/10.1134/S1022795418110133\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1134/S1022795418110133","source_license":"CC0","license_restricted":false}