{"paper_id":"788c0d1c-4deb-45fc-81b2-cad32502ba1e","body_text":"Full Paper\nEffect of Danazol on NK Cells and Cytokines in the Mouse Uterus\nKeywords:\nIntegrin α5,\nDanazol,\nLy49 antigen,\nMacrophage-colony stimulating factor,\nUterine natural killer cells\n2007 Volume 53 Issue 1 Pages 87-94\nDetails\nAbstract\nDanazol, which has been used as a medicine for endometriosis, has a valid effect in pretreatment of patients receiving in vitro fertilization and embryo transfer, although its reproductive mechanism remains unclear. BALB/c mice were subcutaneously injected with danazol for 2 weeks. Blood and uteri were collected and cytokines were assayed. Following danazol treatment, an increase in pregnancy ratio was evident that was accompanied by up-regulation in serum macrophage-colony stimulating factor (M-CSF). RT-PCR analysis revealed that expression of M-CSF and Ly49, a phenotypic marker of natural killer (NK) cells, was up-regulated in the uteri of the danazol-treated mice. In immunohistochemical analysis, M-CSF and Ly49, together with α5 integrin, were clearly detected in the endometrium of the danazol-treated mice with very similar pattern of localization. These results suggest that danazol has an effect to promote pregnancy that induces recruitment of NK cells and a concomitant increase in the expression of M-CSF and α5 integrin in the uterus.\n© 2007 Society for Reproduction and Development\nこの記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。\nhttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja\nFavorites & Alerts\nRecently viewed articles","source_license":"CC0","license_restricted":false}