{"paper_id":"7777455f-21d3-4c18-9c18-bb038090d6ba","body_text":"A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients\nPublic DepositedAdd to collection\nYou do not have access to any existing collections. You may create a new collection.\nDownloadable Content\nDownload PDFCitation\nMLA\nKhalaj, Kasra, et al. A Balancing Act: Rna Binding Protein Hur/ttp Axis In Endometriosis Patients. 2017. https://doi.org/10.17615/zrbc-w664APA\nKhalaj, K., Ahn, S., Bidarimath, M., Nasirzadeh, Y., Singh, S., Fazleabas, A., Young, S., Lessey, B., Koti, M., & Tayade, C. (2017). A balancing act: RNA binding protein HuR/TTP axis in endometriosis patients. https://doi.org/10.17615/zrbc-w664Chicago\nKhalaj, Kasra, Soo Hyun Ahn, Mallikarjun Bidarimath, Yasmin Nasirzadeh, Sukhbir S Singh, Asgerally T Fazleabas, Steven L Young et al. 2017. A Balancing Act: Rna Binding Protein Hur/ttp Axis In Endometriosis Patients. https://doi.org/10.17615/zrbc-w664- Creator\n-\nKhalaj, Kasra\n- Other Affiliation: Department of Biomedical and Molecular Sciences; Queen's University\n-\nAhn, Soo Hyun\n- Other Affiliation: Department of Biomedical and Molecular Sciences; Queen's University\n-\nBidarimath, Mallikarjun\n- Other Affiliation: Department of Biomedical and Molecular Sciences; Queen's University\n-\nNasirzadeh, Yasmin\n- Other Affiliation: Department of Biomedical and Molecular Sciences; Queen's University\n-\nSingh, Sukhbir S.\n- Other Affiliation: Department of Obstetrics and Gynecology; University of Ottawa\n-\nFazleabas, Asgerally T.\n- Other Affiliation: Department of Obstetrics Gynecology and Reproductive Biology; Michigan State University; College of Human Medicine\n-\nYoung, Steven L.\n- School of Medicine, Department of Obstetrics and Gynecology\n-\nLessey, Bruce A.\n- Other Affiliation: Department of Obstetrics and Gynecology; Greenville Health Systems\n-\nKoti, Madhuri\n- Other Affiliation: Department of Biomedical and Molecular Sciences; Queen's University\n-\nTayade, Chandrakant\n- Other Affiliation: Department of Biomedical and Molecular Sciences; Queen's University\n-\nKhalaj, Kasra\n- Abstract\n- Endometriosis, a major reproductive pathology affecting 8-10% of women is characterized by chronic inflammation and immune dysfunction. Human antigen R (HuR) and Tristetraprolin (TTP) are RNA binding proteins that competitively bind to cytokines involved in inflammation including: tumor necrosis factor alpha (TNF-α), granulocyte macrophage colony stimulating factor (GM-CSF), interleukin 6 (IL-6) among others, and stabilize and destabilize them, respectively. The aim of this study was to examine RNA binding protein (RNABP) HuR/TTP axis in endometriosis patients compared to menstrual stage matched healthy fertile controls in hopes of better understanding their contribution to the pathogenesis of endometriosis. Additionally, using a targeted in vitro siRNA approach, we examined whether knock-down of TTP can play a functional role on other RNABPs that competitively bind to inflammatory targets of TTP in both endometriotic and endometrial epithelial cell lines. Our results suggest that RNABPs TTP and HuR are dysregulated in endometriotic lesions compared to matched eutopic patient samples as well endometrium from healthy controls. Silencing of TTP in endometriotic and endometrial epithelial cells revealed differential response to inflammatory cytokines and other RNABPs. Our results suggest potential involvement of HuR/TTP RNA binding protein axis in regulation of inflammation in endometriosis.\n- Date of publication\n- 2017\n- DOI\n- Identifier\n- Publisher DOI: https://doi.org/10.1038/s41598-017-06081-7\n- Onescience id: 694d33fd7494260ad0bb9ab862be7344dbd2a178\n- PMID: 28724967\n- PMCID: PMC5517625\n- Resource type\n- Article\n- Rights statement\n- In Copyright\n- Journal title\n- Scientific Reports\n- Journal volume\n- 7\n- Journal issue\n- 1\n- Language\n- English\n- ISSN\n- 2045-2322\nRelations\n- Parents:\nThis work has no parents.\nItems\n| Thumbnail | Title | Date Uploaded | Visibility | Actions |\n|---|---|---|---|---|\n| PubMedCentral-PMC5517625.pdf | 2017 | Public | Download |","source_license":"CC0","license_restricted":false}